30866_C466395 - SRA

SRX19630321: 30866_C466395
1 ILLUMINA (Illumina HiSeq 2000) run: 1.9M spots, 932.2M bases, 303.6Mb downloads

Design: Genomic DNA libraries are prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, USA) following the manufacturers protocol with the following modifications: input DNA is increased 2-fold, and PCR elongation time is increased to 45 s. DNA quantification and library preparation are carried out on a Hamilton Microlab STAR automated liquid handling system (Hamilton Bonaduz AG, Switzerland). Pooled libraries are quantified using the Kapa Biosystems Library Quantification Kit for Illumina. Libraries are sequenced using Illumina sequencers (HiSeq/NovaSeq) using a 250bp paired end protocol. Reads are adapter trimmed using Trimmomatic 0.30 with a sliding window quality cutoff of Q48

Submitted by: University of Edinburgh

Study: Genomics of Klebsiella species in bovine mastits

PRJNA943144 • SRP426566 • All experiments • All runs

show Abstracthide Abstract

Bovine mastitis is an economically important livestock infection. It can be caused by a range of microorganisms inlcuding Klebsiella. We are using genome sequencing to gain insight into Klebsiella isolates from bovine mastitis such as their phylogeny and genetic determinants of antimicrobial resistance.

Sample:

SAMN33709365 • SRS16999883 • All experiments • All runs

Organism: Klebsiella pneumoniae

Library:

Name: 30866_C466395

Instrument: Illumina HiSeq 2000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 1.9M spots, 932.2M bases, 303.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR23798221	1,933,310	932.2M	303.6Mb	2023-03-10

ID:: 26928115

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