RNA-seq of Caenorhabditis elegans, L3, Replicate 5 - SRA

SRX22239546: RNA-seq of Caenorhabditis elegans, L3, Replicate 5
1 ILLUMINA (Illumina HiSeq 4000) run: 4.9M spots, 971.7M bases, 393Mb downloads

Design: Nematodes in Trizol were subjected to ten freeze-thaw cycles in liquid nitrogen for tissue disruption. RNA was then isolated with the Qiagen RNeasy kit and resuspended in 15 l of RNAse-free water. We used 100 ng of total RNA for mRNA extraction and Illumina library preparation using the Kapa mRNA Hyper prep kit (KK8580). Samples were sequenced on the Illumina HiSeq 4000 platform at the University of Oregon (https://gc3f.uoregon.edu/).

Submitted by: University of Oklahoma

Study: C. inopinata developmental transcriptomics

PRJNA1031217 • SRP468591 • All experiments • All runs

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Transcriptomic data across the L3, L4, and adult stages of Caenorhabditis inopinata and Caenorhabditis elegans with the aim of discovering genes important for body length evolution.

Sample: C_elegans_L3_sample_20

SAMN37931701 • SRS19293702 • All experiments • All runs

Organism: Caenorhabditis elegans

Library:

Name: sample_20

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: Oligo-dT

Layout: PAIRED

Runs: 1 run, 4.9M spots, 971.7M bases, 393Mb

Run	# of Spots	# of Bases	Size	Published
SRR26536569	4,858,662	971.7M	393Mb	2023-10-26

ID:: 30200262

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