RNA-seq of Caenorhabditis elegans, L4, Replicate 4 - SRA

SRX22239551: RNA-seq of Caenorhabditis elegans, L4, Replicate 4
1 ILLUMINA (Illumina HiSeq 4000) run: 4M spots, 797.9M bases, 319.7Mb downloads

Design: Nematodes in Trizol were subjected to ten freeze-thaw cycles in liquid nitrogen for tissue disruption. RNA was then isolated with the Qiagen RNeasy kit and resuspended in 15 l of RNAse-free water. We used 100 ng of total RNA for mRNA extraction and Illumina library preparation using the Kapa mRNA Hyper prep kit (KK8580). Samples were sequenced on the Illumina HiSeq 4000 platform at the University of Oregon (https://gc3f.uoregon.edu/).

Submitted by: University of Oklahoma

Study: C. inopinata developmental transcriptomics

PRJNA1031217 • SRP468591 • All experiments • All runs

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Transcriptomic data across the L3, L4, and adult stages of Caenorhabditis inopinata and Caenorhabditis elegans with the aim of discovering genes important for body length evolution.

Sample: C_elegans_L4_sample_24

SAMN37931705 • SRS19293708 • All experiments • All runs

Organism: Caenorhabditis elegans

Library:

Name: sample_24

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: Oligo-dT

Layout: PAIRED

Runs: 1 run, 4M spots, 797.9M bases, 319.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR26536564	3,989,737	797.9M	319.7Mb	2023-10-26

ID:: 30200267

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