GEO DataSets

(Submitter supplied) The carcinogen aflatoxin is synthesized by a cluster of genes that are regulated by the transcriptional factor, AflR. Most, but not all of these genes, have a consensus binding site for AflR in their 5’ untranslated region. Because aflR resides within the biosynthetic cluster, is has been suggested that it regulates only genes within the cluster. The objective of this study was to identify those genes transcriptionally regulated by AflR and to determine if any of those genes reside outside the aflatoxin biosynthetic cluster. more...

Organism:

Aspergillus parasiticus; Aspergillus flavus

Type:

Expression profiling by array

Platform:

GPL2122

12 Samples

Download data

(Submitter supplied) Objective: Aspergillus flavus aflR, a gene encoding a Zn(II)2Cys6 DNA-binding domain, is an important transcriptional regulator of the aflatoxin biosynthesis gene cluster. Our previous results of GO analysis for the binding sites of AflR in A. flavus suggest that AflR may play an integrative regulatory role. This study aimed to investigate the integrative function of the aflR gene in A. flavus. Design: In this study, we used Aspergillus flavus NRRL3357 as a wild-type strain (WT) and constructed a knockout strain of A. more...

Organism:

Aspergillus flavus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30388

18 Samples

Download data: XLS

(Submitter supplied) Aflatoxins are carcinogenic fungal secondary metabolites. Levels of aflatoxins in agricultural commodities are stringently regulated by many countries. A cluster of genes is responsible for aflatoxin biosynthesis by Aspergillus flavus and other closely related species. Expression of the clustered aflatoxin genes is governed by a complex network of regulatory mechanisms. To better understand the molecular events that are associated with aflatoxin production, transcription profiling by microarray analyses which compared three independent aflatoxigenic A. more...

Organism:

Aspergillus flavus

Type:

Expression profiling by array

Platform:

GPL2122

12 Samples

Download data: TXT

(Submitter supplied) Aflatoxins are toxic and carcinogenic secondary metabolites produced by the fungi Aspergillus flavus and A. parasiticus. In order to better understand the molecular mechanisms that regulate aflatoxin production, the biosynthesis of the toxin in A. flavus and A. parasticus grown in yeast extract sucrose media supplemented with 50 mM tryptophan (Trp) were examined. A. flavus grown in the presence of 50 mM tryptophan was found to have significantly reduced aflatoxin B1 and B2 biosynthesis, while A. more...

Organism:

Aspergillus parasiticus; Aspergillus flavus; Aspergillus flavus NRRL3357

Type:

Expression profiling by array

Platform:

GPL2122

12 Samples

Download data

(Submitter supplied) To better understand the effect of temperature on mycotoxin biosynthesis, RNA-Seq technology was used to profile the Aspergillus flavus transcriptome under different temperature conditions. This approachallowed us to quantify transcript abundance for over 80% of fungal genes including 1,153 genes that were differentially expressed at 30°C and 37°C. Wleven of the 55 secondary metabolite clusters were up-regulated at the lower temperature, including aflatoxin biosynthesis genes, which were among the most highly up-expressed genes. more...

Organism:

Aspergillus flavus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13733

2 Samples

Download data: TXT

(Submitter supplied) Gene expression analysis of A. parasiticus grown under conditions conducive and nonconductive for aflatoxin production was evaluated using glass slide microarrays containing 765 ESTs. Aflatoxins (AF) produced by Aspergillus flavus and A. parasiticus are responsible for approximately $270M in losses annually in the corn, peanut, cotton, and tree nut industries of the United States. In an attempt to develop effective control procedures, research has focused on elucidating the pathway of aflatoxin biosynthesis with the goal of identifying potential targets for intervention. more...

Organism:

Aspergillus flavus; Aspergillus parasiticus

Type:

Expression profiling by array

Platforms:

GPL1393 GPL1394

16 Samples

Download data

(Submitter supplied) Aflatoxins are toxic and carcinogenic polyketide metabolites produced by fungal species, including Aspergillus flavus and A. parasiticus. The biosynthesis of aflatoxins is modulated by many environmental factors, including the availability of a carbon source. The gene expression profile of A. parasiticus was evaluated during a shift from a medium with low concentration of simple sugars, Yeast Extract (YE), to a similar medium with sucrose, Yeast Extract Sucrose (YES). more...

Organism:

Aspergillus parasiticus; Aspergillus flavus

Type:

Expression profiling by array

Platform:

GPL2122

22 Samples

Download data: TXT

(Submitter supplied) Aspergillus flavus is the major producer of carcinogenic aflatoxins in crops worldwide. Natural populations of A. flavus show tremendous variation in aflatoxin production some of which can be attributed to extreme environmental conditions (e.g., drought), differential regulation of the aflatoxin biosynthetic pathway, missing cluster genes or loss-of-function mutations. Understanding the evolutionary processes that generate genetic diversity in A. more...

Organism:

Zea mays; Aspergillus flavus; Aspergillus oryzae

Type:

Genome variation profiling by array

Platform:

GPL13221

19 Samples

Download data: CEL

(Submitter supplied) Gene expression analysis of A. parasiticus grown under conditions conducive and nonconductive for aflatoxin production was evaluated using glass slide microarrays containing the 753 ESTs. A complex regulatory network governs the biosynthesis of aflatoxin. While several genes involved in aflatoxin production are known, their action alone cannot account for its regulation. Arrays of clones from an Aspergillus flavus cDNA library and glass slide microarrays of ESTs were screened to identify additional genes. more...

Organism:

Aspergillus flavus; Aspergillus parasiticus

Type:

Expression profiling by array

Platform:

GPL225

4 Samples

Download data

(Submitter supplied) Diverse expression patterns for secondary metabolism gene clusters from Aspergillus flavus under different environmental conditions and in genetic mutants: Insights into regulation of cyclopiazonic acid along with aflatoxin Species of Aspergillus produce a diverse array of secondary metabolites, and recent genomic analysis predicts that these species have the capacity to synthesize many more compounds. more...

Organism:

Aspergillus oryzae; Zea mays; Aspergillus flavus

Type:

Expression profiling by array

Platform:

GPL8345

75 Samples

Download data: CEL

(Submitter supplied) Aspergillus flavus is a common saprophyte and opportunistic pathogen producing aflatoxin (AF) and many other secondary metabolites. 5-Azacytidine (5-AC), a derivative of nucleoside cytidine, is widely used for studies in epigenetics and cancer biology as an inactivator of DNA methyltransferase and is also used for studying secondary metabolism in fungi. Our previous studies showed that 5-AC affects development and inhibits AF production in A. more...

Organism:

Aspergillus flavus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15956

3 Samples

Download data: TXT

(Submitter supplied) Aflatoxins are carcinogenic secondary metabolites produced by the fungi Aspergillus flavus and Aspergillus parasiticus. Repeated serial mycelial transfer or treatment of A. parasiticus with 5-azacytidine produced mutants with a fluffy phenotype and loss of aflatoxin production. To understand how these treatments affect aflatoxin production and development, we carried out expressed sequence tag (EST)-based microarray assays to identify differentially expressed genes in clones obtained from these treatments. more...

Organism:

Aspergillus flavus; Aspergillus parasiticus

Type:

Expression profiling by array

Platform:

GPL2122

18 Samples

Download data: TAV, XLS

(Submitter supplied) We report here the AflR binding motif of Aspergillus flavus for the first time with the aid of ChIP-seq analysis. Of the 540 peak sequences associated with AflR binding events, 66.8% were located within 2 kb upstream (promoter region) of translational start sites. The identified 18-bp binding motif was a perfect palindromic sequence, 5′-CSSGGGWTCGAWCCCSSG’3′ with S representing G or C and W representing A or T. more...

Organism:

Aspergillus flavus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL23153

2 Samples

Download data: TAR, TXT

(Submitter supplied) Naturally occurring Antisense Transcripts (NATs) compose an emerging group of regulatory RNAs. These regulatory elements appear in all organisms examined, but little is known about global expression of NATs in fungi. Analysis of currently available EST sequences suggests that 352 cis NATs are present in Aspergillus flavus. An Affymetrix GeneChip® microarray containing probes for these cis NATs, as well as all predicted genes in A. more...

Organism:

Zea mays; Aspergillus flavus

Type:

Expression profiling by array

Platform:

GPL7216

6 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Aspergillus oryzae; Aspergillus niger; Aspergillus nidulans

Type:

Expression profiling by array

Platform:

GPL5975

18 Samples

Download data: CEL

(Submitter supplied) The full genome sequencing of the filamentous fungi Aspergillus nidulans, Aspergillus niger and Aspergillus oryzae has opened the possibilities for studying the cellular physiology of these fungi on a systemic level. As a tool to explore this, we are presenting an Affymetrix GeneChip developed for transcriptome analysis of any of the three above-mentioned aspergilli. Transcriptome analysis of triplicate batch cultivations of all three aspergilli on glucose-and xylose media has been performed, and used to validate the performance of the micro array. more...

Organism:

Aspergillus niger; Aspergillus oryzae; Aspergillus nidulans

Type:

Expression profiling by array

Platform:

GPL5975

6 Samples

Download data: CEL

(Submitter supplied) The full genome sequencing of the filamentous fungi Aspergillus nidulans, Aspergillus niger and Aspergillus oryzae has opened the possibilities for studying the cellular physiology of these fungi on a systemic level. As a tool to explore this, we are presenting an Affymetrix GeneChip developed for transcriptome analysis of any of the three above-mentioned aspergilli. Transcriptome analysis of triplicate batch cultivations of all three aspergilli on glucose-and xylose media has been performed, and used to validate the performance of the micro array. more...

Organism:

Aspergillus niger; Aspergillus nidulans; Aspergillus oryzae

Type:

Expression profiling by array

Platform:

GPL5975

6 Samples

Download data: CEL

(Submitter supplied) The full genome sequencing of the filamentous fungi Aspergillus nidulans, Aspergillus niger and Aspergillus oryzae has opened the possibilities for studying the cellular physiology of these fungi on a systemic level. As a tool to explore this, we are presenting an Affymetrix GeneChip developed for transcriptome analysis of any of the three above-mentioned aspergilli. Transcriptome analysis of triplicate batch cultivations of all three aspergilli on glucose-and xylose media has been performed, and used to validate the performance of the micro array. more...

Organism:

Aspergillus oryzae; Aspergillus niger; Aspergillus nidulans

Type:

Expression profiling by array

Platform:

GPL5975

6 Samples

Download data: CEL