GPL24853[Accession] - GEO DataSets

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Items: 3

Select item 2001127521.

Protein microarray epitope mapping of monoclonal antibody 5H2 raised by human immunization with the 4CMenB vaccine formulation containing the fusion antigen NHBA-NMB1030.

(Submitter supplied) The aim of the study was to determine the epitope targeted by 5H2 human Fab directed against NHBA and the crossreactivity aginst a panel of nine different NHBA long and short peptide variants (p2, p3, p5, p10, p17, p20, p21, p24, p29). 5H2 were diluted at 1:2000 and incubated on a non-commercial Protein Microarray platform printed with NHBAp2 specific recombinant protein fragments and full length NHBA of different variants.

Organism:: Homo sapiens; Neisseria meningitidis

Type:: Protein profiling by protein array

Platform:: GPL24853
1 Sample

Download data: TXT

Series

Accession:: GSE112752

ID:: 200112752

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Select item 1000248532.

Neisseria meningitidis Molecular biology Lab, AIMB, GSK Vaccines Protein Microarray

(Submitter supplied) Purified recombinant proteins were diluted at final concentration of 0.5 mg/ul in 40% glycerol and transferred into 384-well microtiterplates. One droplet of 0.2 nL protein solution was deposited per spot on nitrocellulose-coated slides (FAST slides, Schleicher and Schuell) using the non-contact printer Marathon Spotter (Arrayjet, Edinburgh, UK). Printing was performed in a cabinet with controlled humidity and temperature (55-60% and 12 °C respectively), resulting in spots of 90-110 μm in diameter range. more...