Purified recombinant proteins were diluted at final concentration of 0.5 mg/ul in 40% glycerol and transferred into 384-well microtiterplates. One droplet of 0.2 nL protein solution was deposited per spot on nitrocellulose-coated slides (FAST slides, Schleicher and Schuell) using the non-contact printer Marathon Spotter (Arrayjet, Edinburgh, UK). Printing was performed in a cabinet with controlled humidity and temperature (55-60% and 12 °C respectively), resulting in spots of 90-110 μm in diameter range. Each protein was printed in 8 replicates on each slide. Printed protein microarrays were kept at room temperature for 5 hours, dried under a stream of nitrogen and store a 4°C.
Protein microarray epitope mapping of monoclonal antibody 5H2 raised by human immunization with the 4CMenB vaccine formulation containing the fusion antigen NHBA-NMB1030.
Data table header descriptions
ID
Name
common protein name
aminoacid region
the aminoacid region of NHBA long and short peptide variants (p2, p3, p5, p10, p17, p20, p21, p24, p29) spotted on the protein array
