GEO DataSets

(Submitter supplied) cDNA microarray analysis to identify genes regulated by the RNA chaperone, Hfq. Four experiments were performed: 1/ Hfq+ vs Hfq- strains. 269 significantly differentially regulated genes were identified by SAM (Statistical Analysis of Microarrays), of which 120 changed more than 1.5 fold (48 increased and 72 decreased in hfq-). Amongst other genes, these experiments identified significant regulation of the sigma E and sigma 32 regulons. more...

Organism:

Escherichia coli

Type:

Expression profiling by array

Platforms:

GPL3500 GPL2928 GPL4620

21 Samples

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(Submitter supplied) DNA microarray analysis of genes regulated by the alternative sigma factor, sigma E (rpoE). Sigma E-dependent genes were initially identified by comparing a wild type (wt) E. coli K-12 strain that has a low level of sigma E, with a strain over-expressing sigma E (following induction of rpoE from an inducible promoter by IPTG). We monitored changes in gene expression in 4 separate time-courses after induction and used SAM (Statistical Analysis of Microarrays) to identify 75 significantly induced and 8 significantly repressed genes. more...

Organism:

Escherichia coli

Type:

Expression profiling by array

Platforms:

GPL2928 GPL2926 GPL2927

21 Samples

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(Submitter supplied) A microarray with 5376 spot features. Tip configuration: Standard 16-tip Column per Sector: 24 Rows per Sector: 14 Column spacing: 183 um Row spacing: 183 um Protocol: PCR products for 4110 ORFs representing 95.8% of the Escherichia coli K12 MG1655 ORFs were prepared according to Richmond et al (1999; Nucleic Acids Res. 27:3821-35). The products were spotted onto glass slides to make DNA arrays as described in MGUIDE at http://cmgm.stanford.edu/pbrown/mguide/index.html and protocols on http://www.microarrays.org/

Organism:

Escherichia coli

2 Series

11 Samples

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