Similar studies for GEO DataSets (Select 1824) - GEO DataSets

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Items: 20

Select item 18241.

Transcription factors Nanog and Oct4 knockdown effect on embryonic stem cells

Analysis of embryonic stem (ES) cells following RNA interference-mediated depletion of Nanog or Oct4. Nanog and Oct4 are required to maintain the pluripotency and self-renewal of ES cells. Differentially expressed genes scanned for the presence of binding sites for Nanog and Oct4.

Organism:: Mus musculus

Type:: Expression profiling by array, transformed count, 3 protocol sets

Platform:: GPL1261
Series:: GSE4189
14 Samples

Download data: CEL

DataSet

Accession:: GDS1824

ID:: 1824

Select item 2000041892.

The Oct4 and Nanog transcription network that regulates pluripotency in mouse embryonic stem cells

(Submitter supplied) A) We profiled gene expression changes during ES cell differentiation B) We profiled gene expression changes when Pou5f1 or Nanog is knockdown upon RNAi Keywords: Array Based

Organism:: Mus musculus

Type:: Expression profiling by array

Dataset:: GDS1824
Platforms:: GPL1261 GPL3440
32 Samples

Download data: CEL, GPR

Series

Accession:: GSE4189

ID:: 200004189

Select item 2000097753.

RNAi expression experiments on Illumina BeadChip

(Submitter supplied) Mouse embryonic stem cells (mES) were depleted of Klf2, Klf4, and Klf5 by ectopic expression of shRNA. Control RNAi was performed using shRNA against luciferase. At 2 days, 4 days and 6 days post-transfection of shRNA-encoding vectors, cells are harvested for RNA isolation. Keywords: RNAi expression, Mouse ES cells

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6238
18 Samples

Download data

Series

Accession:: GSE9775

ID:: 200009775

Select item 2000097744.

ChIP-chip on custom NimbleGen genomic tiling arrays

(Submitter supplied) Performing Chromatin IP of Klf2, Klf4, Klf5 and p53 in mouse embryonic stem cells with NimbleGen custom genomic tiling arrays, we sought to decipher Klf2, Klf4, Klf5-regulated genes. Keywords: genomic tiling arrays, ES cells

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by genome tiling array

Platform:: GPL6182
15 Samples

Download data: GFF, PROBE

Series

Accession:: GSE9774

ID:: 200009774

Select item 2000095355.

Identifying targets of Klf2, Klf4, Klf5 and p53 in mouse embryonic stem cells

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Expression profiling by array; Genome binding/occupancy profiling by genome tiling array

Platforms:: GPL6238 GPL6182
33 Samples

Download data: GFF, PROBE

Series

Accession:: GSE9535

ID:: 200009535

Select item 2000146546.

Ctr9 Promoter Region Binding

(Submitter supplied) To study the function of Paf1C in mouse ESCs, we generated an ES cell line stably expressing a location and affinity purification (LAP)-tagged Ctr9 fusiuon protein using the bacterial astificial chromosome (BAC)-based TransgeneOmics approach. To investigate whether pluripotency and lineage control genes differentially regulated upon Paf1C depletion are direct targets of the Paf1C, we analyzed the binding of the Ctr9-LAP fusion protein by ChIP-chip and identified 2175 promoter regions that were bound by Ctr9. more...

Organism:: Mus musculus

Type:: Genome variation profiling by genome tiling array

Platform:: GPL5811
1 Sample

Download data: BED, CEL

Series

Accession:: GSE14654

ID:: 200014654

PubMed Similar studies

Select item 2000120787.

Ctr9 knockdown in mouse ES cells

(Submitter supplied) To monitor global transcript changes after Paf1C depletion we transfected ESCs with esiRNA targeting Ctr9 and control esiRNA (Luc).

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
8 Samples

Download data: CEL

Series

Accession:: GSE12078

ID:: 200012078

Select item 2000129998.

Gata3 acts alongside Cdx2 to promote trophoblast gene expression downstream of Tead4 during mouse development

(Submitter supplied) The first lineage decisions during mouse development lead to establishment of embryonic and extraembryonic tissues. The transcription factor Cdx2 plays a central role by repressing pluripotency genes, such as Oct4 and promoting trophoblast fate at the blastocyst stage. Here we show that the transcription factor Gata3 is coexpressed with Cdx2 in the blastocyst and that overexpression of Gata3 in embryonic stem cells is sufficient to induce expression of trophoblast genes. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Datasets:: GDS3948 GDS3949
Platform:: GPL1261
24 Samples

Download data: CEL, CHP

Series

Accession:: GSE12999

ID:: 200012999

Select item 2000129869.

Expression of Cdx2 or Gata3 in R1 mouse embryonic stem cells

(Submitter supplied) To identify whether Cdx2 or Gata3 can activate trophoblast specific gene expression when expressed in R1 ES cells. To assess the dependency of Gata3 activity on Cdx2, Gata3 was also expressed in Cdx2-null ES cells. Keywords: gene expression

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
10 Samples

Download data: CEL, CHP

Series

Accession:: GSE12986

ID:: 200012986

Select item 20001298510.

Differentiation time course of trophoblast stem cells

(Submitter supplied) To characterized the changes in gene expression during the differentiation of TS cells. TS cells can be derived from two time point during embryogenesis, cell lines tested were from each of these time points. Keywords: time course

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
14 Samples

Download data: CEL, CHP

Series

Accession:: GSE12985

ID:: 200012985

Select item 394911.

Cdx2 and Gata3 overexpression effect on R1 embryonic stem cell line

Analysis of R1 embryonic stem (ES) cells overexpressing transcription factor Cdx2 or Gata3 and cultured under trophoblast stem (TS) cell derivation conditions. Gata3-expressing Cdx2-null ES cells also examined. Results provide insight into the roles of Gata3 and Cdx2 in trophoblast development.

Organism:: Mus musculus

Type:: Expression profiling by array, count, 2 genotype/variation, 3 protocol sets

Platform:: GPL1261
Series:: GSE12999
10 Samples

Download data: CEL, CHP

DataSet

Accession:: GDS3949

ID:: 3949

Select item 394812.

Trophoblast stem cell differentiation in vitro

Analysis of trophoblast stem (TS) cell lines TS3.5 and TS6.5 derived from 2 time points during embryogenesis (from blastocyst and E6.5 embryos, respectively) and differentiated over 6 days. Results provide insight into the molecular basis of trophoblast development.

Organism:: Mus musculus

Type:: Expression profiling by array, count, 2 cell line, 2 protocol, 7 time sets

Platform:: GPL1261
Series:: GSE12999
14 Samples

Download data: CEL, CHP

DataSet

Accession:: GDS3948

ID:: 3948

Select item 20003754813.

Expresion profile of MEF reprogrammed with Yamanaka´s factor together with FoxA2 and Gata4

(Submitter supplied) In a pilot experiment to reprogramme MEF into endoderm, we infected MEF with the Yamanaka´s factors (O: Oct4, K: Klf4, S: Sox2, M:Myc), FoxA2 (F) and Gata4 (G). Global gene expression of isolated clones was performed.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6246
13 Samples

Download data: CEL

Series

Accession:: GSE37548

ID:: 200037548

Select item 20000542314.

H9 hES cells vs. universal RNAs

(Submitter supplied) Comparison of human embryonic stem cell transcriptome with universal RNA pool (10 human cell lines) to reveal hES cell-specific gene expression. Keywords: cell type comparison

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL2507
2 Samples

Download data: TXT

Series

Accession:: GSE5423

ID:: 200005423

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20000542215.

Knock-down of three core transcription factors in hEC cells

(Submitter supplied) Three transcription factors were silenced in human embryonic carcinoma cells, OCT4, NANOG, and SOX2. Downstream effects were analysed by means of microarrays to obtain insights into the regulation of self-renewal in these cells. Keywords: RNAi-chip

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL2507
4 Samples

Download data: TXT

Series

Accession:: GSE5422

ID:: 200005422

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20000861716.

Identification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algor

(Submitter supplied) We present a microarray experiment with the same tet-inducible system but with multiple time points within 24 hr to capture early responses to Oct4 suppression. These data were analyzed together with published genome-wide ChIP data. We found that most tentative target genes (TTG) of Oct4 in ES cells were activated by Oct4 and only a few genes were suppressed. The same method was then applied to find target genes of Sox2 and Nanog based mostly on previously published data. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platforms:: GPL4358 GPL2549 GPL2552
40 Samples

Download data: TXT

Series

Accession:: GSE8617

ID:: 200008617

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20000983617.

NFkB target gene microarray during LIF-withdrawal differentiation of mouse embryonic stem cells

(Submitter supplied) An NFkB target gene array (Panomics, Inc., Redwood City, CA) was performed to profile the expression pattern of 107 NFkB-regulated genes in mouse embryonic stem cells. A small scale microarray was carried out using NFkB target gene array kit (Panomics, Inc., Redwood City, CA). A long sense-strand oligonucleotide for each of the 107 human genes that have been previously shown to be regulated by NFkB signaling pathway was spotted in duplicate on the nitrocellulose membrane. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6239
2 Samples

Download data: TIFF

Series

Accession:: GSE9836

ID:: 200009836

Select item 20004708918.

Identification of Oct4-dependent genes in vivo

(Submitter supplied) Transcriptome analysis of Oct4 null and wild type preimplantation mouse embryos by RNA-sequencing

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL14602
18 Samples

Download data: TXT

Series

Accession:: GSE47089

ID:: 200047089

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20004213519.

Expression data from preimplantation mouse embryos

(Submitter supplied) Landmark events occur in a coordinated manner during preimplantation development of the mammalian embryo, yet the regulatory network that orchestrates these events remains largely unknown. We delineated the regulons of Oct4, Sall4, and Nanog using morpholino (MO)-mediated gene knockdowns followed by microarray analysis of pooled embryos.