GEO DataSets

(Submitter supplied) Female Ames dwarf mouse (df/df) with ad libitum access to water and fed 30% less of the standard pelleted diet with respect to the amount consumed by female ad libitum fed df/df mice (LabDiet, PMI Feeds, Inc., St Louis, MO) caged with microisolator filter tops. Mice were killed between 24 to 26 months of age, tissues removed, rapidly frozen on dry ice, and stored in liquid nitrogen. Total liver RNA was isolated from frozen tissue as described (T. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

8 Samples

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(Submitter supplied) Mice were killed between 24 to 26 months of age, tissues removed, rapidly frozen on dry ice, and stored in liquid nitrogen. Total liver RNA was isolated from frozen tissue as described (T. Tsuchiya, J.M. Dhahbi, X. Cui, P.L. Mote, A. Bartke, S.R. Spindler, Physiological Genomics, Submitted). mRNA levels were measured using the Affymetrix mouse U74Av2 array according to standard protocols. After hybridization, arrays were scanned using a Hewlett-Packard GeneArray Scanner. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1261

Platform:

GPL81

31 Samples

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(Submitter supplied) Normal female Ames mouse (+/+ or +/df) with ad libitum access to water and fed 30% less of the standard pelleted diet with respect to the amount consumed by normal female ad libitum fed +/+ and +/df mice (LabDiet, PMI Feeds, Inc., St Louis, MO) caged with microisolator filter tops. Mice were killed between 24 to 26 months of age, tissues removed, rapidly frozen on dry ice, and stored in liquid nitrogen. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

8 Samples

Download data

(Submitter supplied) Female Ames dwarf mouse (+/+ or +/df) with ad libitum access to water and standard pelleted food (LabDiet, PMI Feeds, Inc., St Louis, MO) caged with microisolator filter tops. Mice were killed between 24 to 26 months of age, tissues removed, rapidly frozen on dry ice, and stored in liquid nitrogen. Total liver RNA was isolated from frozen tissue as described (T. Tsuchiya, J.M. Dhahbi, X. Cui, P.L. Mote, A. Bartke, S.R. Spindler, Physiological Genomics, Submitted). mRNA levels were measured using the Affymetrix mouse U74Av2 array according to standard protocols. After hybridization, arrays were scanned using a Hewlett-Packard GeneArray Scanner. Image analysis was performed as described (Cao SX, Dhahbi JM, Mote PL, and Spindler SR. Genomic profiling of short- and long-term caloric restriction effects in the liver of aging mice. Proc Natl Acad Sci U S A 98: 10630-10635, 2001). A more detailed description of the methods can be found in (T. Tsuchiya, J.M. Dhahbi, X. Cui, P.L. Mote, A. Bartke, S.R. Spindler, Physiological Genomics, Submitted). Keywords = Ames dwarf Keywords = mouse Keywords = Affymetrix Keywords = caloric restriction Keywords: parallel sample

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

7 Samples

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(Submitter supplied) Female Ames dwarf mouse (df/df) with ad libitum access to water and standard pelleted food (LabDiet, PMI Feeds, Inc., St Louis, MO) caged with microisolator filter tops. Mice were killed between 24 to 26 months of age, tissues removed, rapidly frozen on dry ice, and stored in liquid nitrogen. Total liver RNA was isolated from frozen tissue as described (T. Tsuchiya, J.M. Dhahbi, X. Cui, P.L. Mote, A. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

8 Samples

Download data

Analysis of livers from growth hormone/insulin-like growth factor-1 signaling defective Ames dwarfs (DF) subjected to caloric restriction (CR). DF and CR in combination additively extend life span. Results provide insight into the mechanisms underlying this additive effect.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 2 protocol sets

Platform:

GPL81

Series:

GSE1093

31 Samples

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(Submitter supplied) Cows were selected from two groups of 12 cows enrolled in a large experiment to assess the effects of ad libitum or restricted intake of moderate-energy diets during the entire dry period on pre-partum metabolism and post-partum metabolism and performance. A corn silage-based diet (26% of diet dry matter) providing 1.59 Mcal/kg during the far-off dry period (first 5 wk of an 8-wk dry period) or providing 1.61 Mcal/kg during the close-up dry period (last 3 wk of the dry period) was fed for ad libitum or restricted intake. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2864

106 Samples

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(Submitter supplied) Mice and diets. One-month-old male mice of the long-lived B6C3F1 strain were purchased from Harlan (Indianapolis, IN) and maintained as described after weaning (15). Mice were housed in groups of 4 per cage and fed a non-purified diet, PMI Nutrition International Product #5001 (Purina Mills, Richmond, IN). At 5 months of age, the mice were individually housed, and randomly assigned to one of two groups, control (CON) or long-term CR (LTCR). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1808

Platform:

GPL81

32 Samples

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Analysis of livers of long-lived B6C3F1 animals fed a diet containing metformin, glipizide, rosiglitazone, or soy isoflavone extract. Results compared to hepatic gene expression profile produced by long-term caloric restriction. Caloric restriction extends the life span of laboratory animals.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 6 agent, 3 protocol sets

Platform:

GPL81

Series:

GSE2431

32 Samples

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(Submitter supplied) Although studies have established that exogenous growth hormone (GH) treatment stimulates growth in fish, its effects on target tissue gene expression are not well characterized. We assessed the effects Posilac® (Monsanto Co., St. Louis, MO), a recombinant bovine somatotropin, on tissue transcript levels. Transcript abundance was measure in liver and muscle using the GRASP 16 K cDNA microarray. A selection of the genes identified as altered with the microarray, and also transcripts for insulin-like growth factors, growth hormone receptors (GHR) and myostatins were measured by realtime PCR in the liver, muscle, brain, kidney, intestine, stomach, gill and heart. more...

Organism:

Oncorhynchus mykiss; Salmo salar

Type:

Expression profiling by array

Platform:

GPL3976

16 Samples

Download data: TXT

(Submitter supplied) Pairwise comparison of gene expression in pools (n=14) of wildtype 4 week old male Vs NaS1-/- knockout mice. Keywords: normal Vs mutant

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1867

4 Samples

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(Submitter supplied) The aim of this study is to obtain the gene expression profiles of the liver of young growing rats after mild restriction of food intake for one week or one month.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL341

20 Samples

Download data: CEL

(Submitter supplied) Background: Calorie restriction (CR) is the only intervention known to extend lifespan in a wide range of organisms, including mammals. However, the mechanisms by which it regulates mammalian aging remain largely unknown and the involvement of the TOR and Sirtuin pathways (which regulate aging in lower organisms) remain controversial. Femaleness is a second phenotype generally associated with longevity but the relationship between sex-biased and CR-induced gene expression remains undetermined. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6761

23 Samples

Download data: GPR

(Submitter supplied) To provide a robust understanding of a transcriptomic change by short-term CR at body fat of mice, we applied three serial strengths of CR to mice including 15%, 30%, and 45% reduction of carbon source. Using Affymetrix mouse 1.0 ST array platform, we obtained and analyzed the transcriptome data for significantly changed genes in expression. Here, we identified 446 genes and categorized the genes based on their biological roles. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

12 Samples

Download data: CEL

(Submitter supplied) Caloric restriction (CR) prolongs lifespan, yet the mechanisms by which it does so remain poorly understood. Under CR, mice self-impose chronic cycles of 2-hour-feeding and 22-hour-fasting, raising the question whether calories, fasting, or time of day are causal. We show that 30%-CR is sufficient to extend lifespan 10%; however, a daily fasting interval and circadian-alignment of feeding act together to extend lifespan 35% in male C57BL/6J mice. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

288 Samples

Download data: XLSX

(Submitter supplied) The hypothalamus has recently emerged as a key regulator of metabolism and aging in mammals. We have examined the impact of targeted disruption of hypothalamic hypophysiotropic peptide: Growth Hormone-releasing Hormone (GHRH) in mice on longevity, and the putative mechanisms of delayed aging. GHRH knockout (KO) mice are remarkably long-lived and in comparison to genetically normal (wild type) animals exhibiting major shifts in the expression of genes related to xenobiotic detoxification, stress resistance, and insulin signaling. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL

(Submitter supplied) The aim of the current work was to evaluate the ovarian transcriptome in Ames dwarf (df/df) mice. df/df mice have a delayed ovarian aging compared to normal (N) mice. Although a high number of genes were differentially expressed during aging of N mice, only a small fraction of these changed with aging in df/df mice. These alterations involved more than 500 categorized biological processes. The majority of these biological processes, including inflammatory/immune responses, were up-regulated with aging in N mice, while old df/df mice were characterized by down-regulation of these same processes in comparison to age matched N mice. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

20 Samples

Download data: GTF, TXT

(Submitter supplied) Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Normal fed animals were fed before and during the 48h experiment with standard food ad libitum. Sugar fed animals were fed before the experiment with standard food ad libitum and during the 48h experiment with 50% (w/v) sugar solution (40% glucose, 10% sucrose). Mice were killed by CO2 asphyxiation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL765

60 Samples

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(Submitter supplied) Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Normal fed animals were fed before and during the 48h experiment with standard food ad libitum. Starved animals were fed before the experiment with standard food ad libitum and were starved during the 24h experiment. Mice were killed by CO2 asphyxiation. The livers were snap-frozen in liquid nitrogen and stored at -80 degrees C. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL765

24 Samples

Download data

(Submitter supplied) Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Normal fed animals were fed before and during the 48h experiment with standard food ad libitum. Starved animals were fed before the experiment with standard food ad libitum and were starved during the 48h experiment. Mice were killed by CO2 asphyxiation. The livers were snap-frozen in liquid nitrogen and stored at -80 degrees C. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL765

24 Samples

Download data