GEO DataSets

(Submitter supplied) Peripheral blood lymphocytes from a total of 15 healthy individuals without history of cancer (NoCa) were immortalized with Epstein-Barr virus. Cells were exposed to mock treatment (Mock), ultra-violet radiation (UV), or ionizing radiation (IR). For UV radiation treatment, cells were exposed to 10 J/m^2 and harvested for RNA 24 hours later. For IR treatment, cells were exposed to 5 Gy of IR and harvested for RNA 4 hours later. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS913

Platform:

GPL8300

45 Samples

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Analysis of peripheral blood lymphocytes exposed to ultraviolet (10 J/m^2) or ionizing radiation (5 Gy). Lymphocytes obtained from 15 healthy individuals from ages 21 to 36 years and immortalized with Epstein-Barr virus. Results provide insight into transcriptional response to DNA damage.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 agent, 15 individual sets

Platform:

GPL8300

Series:

GSE1977

45 Samples

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(Submitter supplied) Rationale: During excessive pressure or overload, cardiac cells are subjected to increased mechanical stress. We investigate how the stress response of cardiac cells to mechanical stress can be compared to genotoxic stresses induced by DNA damaging agents. Methods: Cultures enriched for cardiomyocytes and cultures of fibroblasts were derived from ventricles of neonatal rat hearts. In three independent experiments, both cell cultures were subjected to mechanical stress (cyclic stretch at 60 cycles/min), UV irradiation (10 J/m2), or X-rays (8.5 Gy). more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL85

35 Samples

Download data: CEL, EXP

(Submitter supplied) The cellular response to DNA damage is vital for maintaining genomic stability and preventing undue cell death or cancer formation. The DNA damage response (DDR), most robustly mobilized by double-strand breaks (DSBs), rapidly activates an extensive signaling network that affects numerous cellular systems, leading to cell survival or programmed cell death. A major component of the DDR is the widespread modulation of gene expression. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

75 Samples

Download data: CEL, CHP

(Submitter supplied) Time series for gene expression changes following 3 Gy and 10 Gy of ionizing radiation exposure. Keywords: time-course

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS479

Platform:

GPL91

12 Samples

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Temporal analysis of effect of 3 Gy and 10 Gy ionizing radiation (IR) exposure on lymphoblastoid cells. Various time points up to 24 hours examined.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 dose, 2 stress, 6 time sets

Platform:

GPL91

Series:

GSE701

12 Samples

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(Submitter supplied) The purpose of the study was to identify new PAK1 targets in response to ionizing radiation with putative role in the DNA damage response. We examined the effect of IR on the gene expression patterns in the murine embryonic fibroblasts with or without Pak1 using microarray.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6096

12 Samples

Download data: CEL

(Submitter supplied) DNA damage caused by UV radiation initiates cellular recovery mechanisms, which involve activation of DNA damage response pathways, cell cycle arrest and apoptosis. To assess cellular transcriptional responses to UVC-induced DNA damage we compared time course responses of human skin fibroblasts to low and high doses of UVC radiation known to induce a transient cellular replicative arrest or apoptosis, respectively. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS400

Platform:

GPL8300

20 Samples

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(Submitter supplied) The transcriptional response to 10 J/m2 of UV-light (254 nm) was assessed with HGU95AV2 Affymetrix probe arrays at 6, 12, 18 and 24 hours after exposure. Mock-treated samples were assessed at 6 and 24 hours. Keywords: time-course

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL8300

12 Samples

Download data

(Submitter supplied) The transcriptional response to 50 J/m2 of UV-light (254 nm) was assessed with HGU95AV2 Affymetrix probe arrays at 6, 12, 18 and 24 hours after exposure. Mock-treated samples were assessed at 6 and 24 hours. Keywords: time-course

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL8300

12 Samples

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Temporal analysis of differences in WS1 human skin fibroblast gene expression response to low (10 J/m2; induces transient cellular replicative arrest) or high (50 J/m2; induces apoptosis) doses of short wavelength UV radiation (UVC; 254 nm).

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 protocol, 4 time sets

Platform:

GPL8300

Series:

GSE713

20 Samples

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(Submitter supplied) Cell cycle arrest and transcriptional responses to ionizing radiation (IR)-induced DNA damage were quantified in telomerase-expressing human diploid fibroblasts. Assays of clonal expansion established 1.5 Gy IR as the D0 dose in three fibroblast lines and this dose was used in all subsequent analyses. Fibroblasts exhibited >90% arrest of progression from G2 to M at 2 h and from G1 to S at 6 and 12 h post-IR. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL885 GPL887

36 Samples

Download data: TXT

(Submitter supplied) The ionizing radiation (IR) dose that yields 20% survival (D20) of Shewanella oneidensis MR-1 is lower by factors of 20 and 200 than for Escherichia coli and Deinococcus radiodurans, respectively. Transcriptome analysis was used to identify the genes of MR-1 responding to 40 Gy (D20). We observed the induction of 170 genes and repression of 87 genes in MR-1 during a one-hour recovery period after irradiation. more...

Organism:

Shewanella oneidensis MR-1

Type:

Expression profiling by array

Platform:

GPL3253

20 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL16770 GPL14550

56 Samples

Download data: TXT

(Submitter supplied) Ionizing Radition is known to cause cell damage. Human peripheral blood mononuclear cells have long been used to study radiation induced gene expression profiling. Whithin this study we evaluated gene and microRNA expression alterations of human PBMC irradiated with 60 Gy g-ray. Cells were cultured for 2, 4 and 20h after irradiation before RNA was isolated and Agilent Human microRNA Microarrays was performed.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL16770

28 Samples

Download data: TXT

(Submitter supplied) Ionizing Radition is known to cause cell damage. Human peripheral blood mononuclear cells have long been used to study radiation induced gene expression profiling. Within this study we evaluated gene and microRNA expression alterations of human PBMC irradiated with 60 Gy g-ray. Cells were cultured for 2, 4 and 20h after irradiation before RNA was isolated and Agilent whole human GenomeOligo Microarray was performed.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL14550

28 Samples

Download data: TXT

(Submitter supplied) Peripheral blood lymphocytes from a total of 57 patients were immortalized with Epstein-Barr virus. Fourteen radiation-therapy patients suffered unusual levels of radiation toxicity (RadS). Thirteen radiation-therapy patients with limited toxicity (RadC) were enrolled as controls. Fifteen patients diagnosed with skin cancer before age 40 (SkCa) were used as a second group of controls. Fifteen healthy subjects without any history of cancer (NoCa) were matched to the skin cancer patients and used as a third group of controls. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS968

Platform:

GPL8300

171 Samples

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Analysis of UV and IR irradiated lymphoblastoid cell lines derived from the peripheral blood of patients with acute radiation toxicity. Samples taken 2 months after the completion of radiation therapy. Results provide insight into the role of the response to DNA damage in radiation toxicity.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 agent, 4 disease state, 57 individual sets

Platform:

GPL8300

Series:

GSE1725

171 Samples

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(Submitter supplied) Genotoxic damage is known to disrupt cellular functions and is lethal if not repaired properly. We compare the transcriptional programs activated in response to genotoxic DNA damage induced by ionizing radiation (IR) in pre-B cells from mice deficient in various DNA damage response (DDR) genes. We used microarrays to detail the global gene expression of mutated cells compared to WT cells, with and without exposure to the DNA damaging agent ionizing radiation (IR), and identified differentially-regulated genes and associated pathways responding to the damage.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

42 Samples

Download data: CEL, CHP

(Submitter supplied) DNA repair is an essential cellular process required to maintain genomic stability. Every cell is subjected to thousands of DNA lesions daily under normal changes in transcription. Transcription is a primary process where protein amount and function can be regulated. One aspect of the transcriptional IR response that little is known about on a whole genome basis is alternative transcription. These investigations focus on the response to IR at the exon level in human cells but also at the whole gene level. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5175

132 Samples

Download data: CEL