GEO DataSets

(Submitter supplied) Background Our objective was to compare mammary tissue gene expression profiles during a Streptococcus uberis (S. uberis) mastitis challenge between lactating cows subjected to dietary-induced negative energy balance (NEB; n = 5) and cows fed ad libitum to maintain positive energy balance (PEB; n = 5). The NEB cows were feed-restricted to 60% of calculated net energy for lactation requirements for 7 d, and cows assigned to PEB were fed the same diet for ad libitum intake. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL8776

40 Samples

Download data: TXT

(Submitter supplied) The objective was to evaluate the effect of nutrient restriction and intramammary lipopolysaccharide (LPS) challenge on mammary gland (MG) gene expression in early lactation cows. At 24 ± 3 d in milk, multiparous cows were either allowed to continue ad libitum intake of a lactation diet (CON, n = 6), or the ration was diluted with barley straw (48% DM) for 4 d (RES, n = 6). On d 3, one healthy rear mammary quarter was infused with 50 µg of LPS. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11648

12 Samples

Download data: TXT

(Submitter supplied) Mastitis in dairy cows is one of the most costly and prevalent diseases affecting dairy cows world wide. Insight in the molecular regulation of the host immune response to an E. coli infection, could help to develop new strategies to prevent cattle from E. coli infection. Here we performed a gene-expression analysis from udder tissue exposed to a controlled E. coli infection at T=24h post infection (p.i.) representing the acute phase response and at T=192h p.i. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

49 Samples

Download data: CEL

(Submitter supplied) Direct transcriptomics comparison corroborated by functional and gene network analyses of pre-weaned bovine mammary parenchyma and fat pad shed light on potential cross-talk between these developing tissues. Transcriptomic characterization of mammary parenchyma and fat pad and their interaction is still incomplete. In the present experiment, the molecular epithelial-fat pad cross-talk during mammary development was assessed by transcript profiling and functional analyses. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL8776

41 Samples

Download data: GPR

(Submitter supplied) Establishment of an in vitro system to explore molecular mechanisms of mastitis susceptibility in cattle by comparative expression profiling of Escherichia coli and Staphylococcus aureus inoculated primary cells sampled from cows with different genetic predisposition for somatic cell score Primary bovine mammary gland epithelial cells (pbMEC) were sampled from the udder parenchyma of cows that were selected for high and low mastitis susceptibility by applying a marker assisted selection strategy considering QTL and molecular marker information of a repetitively confirmed QTL for SCS in the telomeric region on BTA18 The cells were cultivated and subsequently inoculated with heat inactivated mastitis pathogens Escherichia coli and Staphylococcus aureus, respectively. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Dataset:

GDS4437

Platform:

GPL2112

88 Samples

Download data: CEL

Mammary gland epithelial cells of cows with favorable somatic cell score (SCS) QTL allele Q and unfavorable allele q after S. aureus or E.coli exposure for up to 24 hours. SCS is an indicator of milk quality and udder health. Results provide insight into molecular basis of mastitis susceptibility.

Organism:

Bos taurus

Type:

Expression profiling by array, count, 2 genotype/variation, 3 protocol, 3 time sets

Platform:

GPL2112

Series:

GSE24560

88 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

30 Samples

Download data: CEL

(Submitter supplied) By comparison of the transcriptome profiles of udder quarters neighboring to infected quarters and healthy udder tissue we analyse gene expression in the late stage of infection with E. coli 1303. Differentially expressed genes and transcription factors regulating coexpressed genes identified by SOTA clustering were used to identify key genes that define systemic reactions to infection with E. coli 1303. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

10 Samples

Download data: CEL

(Submitter supplied) By comparison of the transcriptome profiles of infected and healthy udder tissue we analyse gene expression in the late stage of infection with E. coli 1303. Differentially expressed genes and transcription factors regulating coexpressed genes identified by SOTA clustering were used to identify key genes that define late stage of infection with E. coli 1303.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

10 Samples

Download data: CEL

(Submitter supplied) By comparison of the transcriptome profiles of infected and healthy udder tissue we analyse gene expression in the early stage of infection with E. coli 1303. Differentially expressed genes and transcription factors regulating coexpressed genes identified by SOTA clustering were used to identify key genes that define early stage of infection with E. coli 1303. Keywords: disease state analysis

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

10 Samples

Download data: CEL

(Submitter supplied) Mastitis in dairy cattle can result from infection by a range of microorganisms but is principally caused by coliform bacteria and gram positive bacteria such as Staphylococcus aureus (S. aureus). The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL6082

36 Samples

Download data: GPR

(Submitter supplied) Mastitis in dairy cattle can result from infection by a range of microorganisms but is principally caused by coliform bacteria and gram positive bacteria such as Staphylococcus aureus (S. aureus). The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL6082

12 Samples

Download data: GPR

(Submitter supplied) Mastitis in dairy cattle can result from infection by a range of microorganisms but is principally caused by coliform bacteria and gram positive bacteria such as Staphylococcus aureus (S. aureus). The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL6082

12 Samples

Download data: GPR

(Submitter supplied) Mastitis in dairy cattle can result from infection by a range of microorganisms but is principally caused by coliform bacteria and gram positive bacteria such as Staphylococcus aureus (S. aureus). The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL6082

12 Samples

Download data: GPR

(Submitter supplied) The benefit of treatment in mild to moderate cases of E. coli mastitis in dairy cows remains a topic of discussion. We investigated the effect of intramammary treatment with Cefapirin + Prednisolone compared to Cefapirin only on gene expression profiles in experimentally induced E. coli mastitis.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

20 Samples

Download data: CEL

(Submitter supplied) Increased energy demands to support lactation, coupled with lowered feed intake capacity results in negative energy balance (NEB) and is typically characterized by extensive mobilization of body energy reserves in the early postpartum dairy cow. The catabolism of stored lipid leads to an increase in the systemic concentrations of nonesterified fatty acids (NEFA) and -hydroxy butyrate (BHB). Oxidation of NEFA in the liver result in the increased production of reactive oxygen species and the onset of oxidative stress and can lead to disruption of normal metabolism and physiology. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL18534

10 Samples

Download data: CEL

(Submitter supplied) Mastitis is an inflammation of the mammary gland (MG), usually due to bacterial infection. Although considerable attention has been paid to this economically important disease, the early stages of the host response remain poorly defined. In particular, it is unclear how mammary epithelial cells (MEC), a first barrier against pathogens, respond to infection. Indeed, it is difficult to differentiate between the contributions of MEC and infiltrating immune cells to gene expression profiles of mammary tissue during mastitis. more...

Organism:

Capra hircus; Ovis aries

Type:

Expression profiling by array

Platform:

GPL10427

20 Samples

Download data: TXT

(Submitter supplied) Background: The liver is central to most economically important metabolic processes in cattle. However, the changes in expression of genes that drive these processes remain incompletely characterised. RNA-seq is the new gold standard for whole transcriptome analysis but so far there are no reports of its application to analysis of differential gene expression in cattle liver. We used RNA-seq to study differences in expression profiles of hepatic genes and their associated pathways in individual cattle in either mild negative energy balance (MNEB) or severe negative energy balance (SNEB). more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL9323

11 Samples

Download data: TXT

(Submitter supplied) The effects of a negative energy balance induced by a nutritional restriction on mammary gland were evaluated using the 4x44K bovine microarray from Agilent

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11648

12 Samples

Download data: TXT

(Submitter supplied) Effect of feed restriction in mid lactation Holstein (H) and Montbéliarde (M) cows on mammary glande miRNA profil.

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL23295

24 Samples

Download data: TXT