GEO DataSets

(Submitter supplied) There is a wide diversity of potential applications for direct electron transfer from electrodes to microorganisms, which might be better optimized if the mechanisms for this novel electrode-biofilm interaction were further understood. Geobacter sulfurreducens is one of the few microorganisms available in pure culture that is known to be capable of directly accepting electrons from a negatively poised electrode. more...

Organism:

Geobacter sulfurreducens

Type:

Expression profiling by array

Platform:

GPL4897

6 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Geobacter sulfurreducens

Type:

Expression profiling by array

Platform:

GPL4897

12 Samples

Download data: GPR

(Submitter supplied) There is a wide diversity of potential applications for direct electron transfer from electrodes to microorganisms, which might be better optimized if the mechanisms for this novel electrode-biofilm interaction were better understood. Geobacter sulfurreducens is one of the few microorganisms available in pure culture that is known to be capable of directly accepting electrons from a negatively poised electrode. more...

Organism:

Geobacter sulfurreducens

Type:

Expression profiling by array

Platform:

GPL4897

6 Samples

Download data: GPR

(Submitter supplied) G. sulfurreducens can generate electricity from the oxidation of organic compounds. This is because it can take electrons from organic compounds and ship them out to the outer surface of the cell where they can then be deposited on various insoluble electron acceptors including electrodes. Cells attatched to the surface of an electrode oxidize acetate and and deposit the electrons derived from acetate onto the surface of the electrode after which they can travel through an electrical circuit, producing a current. more...

Organism:

Geobacter sulfurreducens

Type:

Expression profiling by array

Platform:

GPL4897

6 Samples

Download data: GPR

(Submitter supplied) OmcF is a monoheme c-type cytochrome in Geobacter sulfurreducens. An OmcF-deficient mutant is impaired in iron reduction and electricity production. Heme staining revealed altered expression of several c-type cytochromes in the OmcF-deficient mutant. Whole genome DNA microarray analysis was performed in order to determine which genes demonstrate altered expression in the OmcF-deficient mutant compared to wildtype. more...

Organism:

Geobacter sulfurreducens

Type:

Expression profiling by array

Platform:

GPL4897

6 Samples

Download data: GPR

(Submitter supplied) Microtoming Coupled with Microarray Analysis to Evaluate Potential Differences in the Metabolic Status of Geobacter sulfurreducens at Different Depths in Anode Biofilms Differences in the Metabolic Status of Geobacter sulfurreducens at Different Depths in A Current Producing Biofilm Further insight into the metabolic status of cells within anode biofilms is essential for understanding the functioning of microbial fuel cells and developing strategies to optimize their power output. more...

Organism:

Geobacter sulfurreducens

Type:

Expression profiling by array

Platform:

GPL8797

2 Samples

Download data: CEL, CHP

(Submitter supplied) The formation of electroactive biofilms is a crucial process for the generation of bioelectricity and bioremediation. G. sulfurreducens is a dissimilatory metal-reducing microorganism that can couple oxidation of organic matter with extracellular electron transfer to different insoluble electron acceptors. It has the capability to form biofilms in insoluble metal oxides and electroconductive biofilms in electrodes in bioelectrochemical systems. more...

Organism:

Geobacter sulfurreducens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33036

8 Samples

Download data: TXT

(Submitter supplied) The ability of Geobacter species to readily donate electrons to extracellular electron acceptors makes the study of their physiology not only important for the understanding of environmental processes, but also for industrial applications such as bioelectronics and electrosynthesis. Studies in G. sulfurreducens have shown that outer surface components, such as c-type cytochromes and conductive type IV pili play an important role in direct electron transfer to extracellular electron acceptors such as Fe(III) oxides and electrodes. more...

Organism:

Geobacter metallireducens GS-15

Type:

Expression profiling by array

Platform:

GPL15983

8 Samples

Download data: PAIR

(Submitter supplied) The conductive pili of Geobacter sulfurreducens are essential for optimal extracellular electron transfer to Fe(III) and long-range electron transport through current-producing biofilms. The KN400 strain of G. sulfurreducens reduces poorly crystalline Fe(III) oxide more rapidly than the more extensively studied DL-1 strain. Deletion of the gene for PilA, the structural pilin protein, in strain KN400 inhibited Fe(III) oxide reduction. more...

Organism:

Geobacter sulfurreducens KN400

Type:

Expression profiling by array

Platform:

GPL17282

8 Samples

Download data: CALLS, PAIR

(Submitter supplied) Previous analysis of gene transcript levels of Geobacter species in groundwater during in situ bioremediation of a uranium-contaminated aquifer detected expression of genes encoding superoxide dismutase (sodA) and cytochrome d ubiquinol oxidase (cydA), proteins known to be involved in the response to oxidative stress in other microorganisms. In order to further elucidate gene expression patterns that could be attributed to oxygen exposure, G. more...

Organism:

Geotalea uraniireducens; Geotalea uraniireducens Rf4

Type:

Expression profiling by array

Platform:

GPL6566

6 Samples

Download data: GPR

(Submitter supplied) Geobacter sulfurreducens is capable of reducing Pd(II) to Pd(0) using pyruvate as an electron donor; however, the biochemical and genetic mechanisms involved in this process have not been described. In this work, we carried out transcriptome profiling analyes to identify genes involved in Pd(II) reduction in this bacteria. Our results showed that 254 genes were upregulated while 142 were downregulated during Pd(II) reduction. more...

Organism:

Geobacter sulfurreducens PCA

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24894

4 Samples

Download data: TXT

(Submitter supplied) Geobacteraceae transfer electrons from a donor such as acetate to an electron acceptor such as Fe(III) or U(VI). Geobacter uraniireducens is found in uranium-contaminated sites and plays an important role in in situ bioremediation. In this experiment, gene expression was compared between G. uraniireducens cultures grown in sediments from a uranium contaminated site amended with acetate and cultures grown in acetate/fumarate medium. more...

Organism:

Geotalea uraniireducens Rf4

Type:

Expression profiling by array

Platform:

GPL6566

6 Samples

Download data: TXT

(Submitter supplied) In this study, expression analysis using electrochemically synthesized Combimatrix 12K DNA microarrays was validated in G sulfurreducens. Growth under nitrogen fixing conditions and growth in ammonium amended medium, respectively, were the experimental and control conditions. Expression analysis using this platform was also compared to expression analysis on whole cDNA microarrays. Keywords: two-condition comparison

Organism:

Geobacter sulfurreducens

Type:

Expression profiling by array

Platform:

GPL4895

6 Samples

Download data: GPR

(Submitter supplied) In this experiment two strains were compared. The control strain was the wild type strain containing the plasmid, pCD341. pCD341 is an inducible expression vector, a plasmid which can be used to make a bacterium produce a protein of interest. It is called inducible, because the protein of interest will only be produced if an inducer, in this case, IPTG, is present. In the control strain pCD341 is "empty", meaning the gene encoding the protein of interest has not been inserted into the vector, so when you add IPTG nothing will be produced. more...

Organism:

Geobacter sulfurreducens

Type:

Expression profiling by array

Platform:

GPL4897

6 Samples

Download data: GPR

(Submitter supplied) The expression profile of adapted G. sulfurreducens strain T9-1 grown with 40 mM fumarate without any other substrate versus the expression profile of G. sulfurreducens PCA (wild type) grown with 10 mM acetate and 40 mM fumarate

Organism:

Geobacter sulfurreducens PCA

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30660

6 Samples

Download data: TXT

(Submitter supplied) In the platform data table, the following FEAT_Name values are of controls with additional description: FEAT_NAME Description RCP1_AF168390 A. thaliana root cap 1 protein (RCP1)-Control rbcL_U91966 A. thaliana ribulose-1,5-bisphosphate (rbcL)-control Cab_X56062 A. thaliana chlorophyll a/b-binding protein (Cab)-control rca_M86720 A. thaliana RUBISCO activase (RCA)-control TIM_AF247559 A. thaliana triosephosphate isomerase (TIM)-Control NAC1_AF198054 A. more...

Organism:

Geobacter sulfurreducens PCA

2 Series

33 Samples

Download data: TXT

(Submitter supplied) Geobacter sulfurreducens was originally considered a strict anaerobe. However, this bacterium was later shown to not only tolerate but also to use oxygen as terminal electron acceptor. Research performed has so far only revealed the general ability of G. sulfurreducens to reduce oxygen, but the oxygen consumption rate has not been quantified, nor has evidence been provided as to how the bacterium achieves oxygen consumption. more...

Organism:

Geobacter sulfurreducens

Type:

Expression profiling by array

Platform:

GPL26004

12 Samples

Download data: TXT

(Submitter supplied) Previous studies demonstrated that an outer membrane c-type cytochrome, OmcB (GSU_2737), was involved in Fe(III) reduction in Geobacter sulfurreducens. An OmcB-deficient mutant was greatly impaired in its ability to reduce both soluble and insoluble Fe(III). Reintroducing omcB restored the capacity for Fe(III) reduction at a level proportional to the level of OmcB production. Here, we report that the OmcB-deficient mutant gradually adapted to grow on soluble Fe(III) but not insoluble Fe(III). more...

Organism:

Geobacter sulfurreducens; Geobacter sulfurreducens PCA

Type:

Expression profiling by array

Platform:

GPL5768

11 Samples

Download data: TAV