GEO DataSets

(Submitter supplied) Comparisons of gene expression profiles between testis and ovary of juvenile and wild brooders were made through a cDNA microarray in the black tiger shrimp (Penaeus monodon). Differentially expressed genes were identified through the microarray analysis, and the microarray results were confirmed by real-time PCR. Selected genes were further characterized.

Organism:

Penaeus monodon

Type:

Expression profiling by array

Platform:

GPL10413

9 Samples

Download data: GPR

(Submitter supplied) Identification of testis-relevant genes using in silico analysis from testes ESTs and cDNA microarray in the black tiger shrimp (Penaeus monodon) A total of 4,803 ESTs from the testis library were unidirectionally sequenced and analyzed in silico using a customizable data analysis package, ESTplus. A total of 2,702 unique sequences from 424 contigs and 2,278 singletons were identified. Approximately, 1,134 sequences from the total of 2,702 unique sequences (contigs and singletons) are homologous to genes with known functions. more...

Organism:

Penaeus monodon

Type:

Expression profiling by array

Platform:

GPL9679

3 Samples

Download data: GPR

(Submitter supplied) To construct the first in-house version of cDNA microarrays for P. monodon, the ovary and testes EST libraries were used as templates for reproduction-specific cDNA microarray (ReproArray) fabrication. ReproArray was used to analyze large-scale gene expression and to demonstrate capacity for studies of reproduction-relevant genes. Keywords: time couse

Organism:

Penaeus monodon

Type:

Expression profiling by array

Platform:

GPL7049

3 Samples

Download data: GPR

(Submitter supplied) Comparisons of gene expression profiles between ovaries of before (day 0) and after eyestalk ablation (days 1, 4 and 7) of domesticated 14-month-old black tiger shrimp (Penaeus monodon) were made using a cDNA microarray. Differentially expressed genes were identified through the microarray analysis, and the microarray results were confirmed by real-time PCR. Selected genes were further characterized.

Organism:

Penaeus monodon

Type:

Expression profiling by array

Platform:

GPL10413

8 Samples

Download data: GPR

(Submitter supplied) The study aimed to determine effect of polychaetes as a shrimp feed on male reproductive maturation at transcriptional level through a cDNA microarray in the black tiger shrimp (Penaeus monodon). Thus, the experiment was to compare transcriptomic profiles of two different parts of reproductive organs, namely testes (TT) and vas deferens (VD), of domesticated 17-month-old between two different feeds, namely commercial pellet and polychaetes after feeding for one month. more...

Organism:

Penaeus monodon

Type:

Expression profiling by array

Platform:

GPL22536

16 Samples

Download data: GPR

(Submitter supplied) Background: Aquaculture of the black tiger prawn Penaeus monodon remains severely constrained by an almost total dependence on wild-caught broodstock. Reliance on wild-caught broodstock stems, for the most part, from reduced reproductive potential of captive-reared females. Reproductive performance of captive-reared females is usually characterised by longer latency period, lower egg production, egg hatch rates and post-larval survivorship compared with their wild-caught counterparts. more...

Organism:

Penaeus monodon

Type:

Expression profiling by array

Platform:

GPL14373

38 Samples

Download data: TXT

(Submitter supplied) Identification and annotation of all the genes in the sequenced Drosophila genome is a work in progress. Wild-type testis function requires many genes and is thus of potentially high value for the identification of transcription units. We therefore undertook a survey of the repertoire of genes expressed in the Drosophila testis by computational and microarray analysis. We generated 3141 high-quality testis expressed sequence tags (ESTs). more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL5

8 Samples

Download data

(Submitter supplied) The array was assembled using 1,681 testes ESTs, representing 1,527 Drosophila melanogaster EST sequences and 144 anonymous clones from the bs collection (CLONE_ID). An aliquot of the same DNA preparation used in the sequencing reactions (GB_ACC), diluted 2/50 in TE, provided material for the PCRs. Inserts were PCR amplified (1X PCR buffer [Life Technologies], 1.5 mM MgCl2, 0.2 mM dNTPs, 1 µM T7 22-mer primer, 1 µM T3 20-mer primer, 2 µl of plasmid DNA dilution [approximately 10 ng], 0.035 U/µl recombinant Taq DNA polymerase [Life Technologies], in a volume of 100 µl, cycled 94C 2 min, 30X[94 C 0.5 min, 55 C 0.5 min, 2.5 min 72 C], 10 min 72 C in a PTC-225 DNA Engine Tetrad [MJ Research]), and successful amplification was confirmed by agarose gel electrophoresis (PCR-QC: passed = product present, failed = no product). more...

Organism:

Drosophila melanogaster

1 Series

8 Samples

Download data

(Submitter supplied) This study was performed to validate the newly developed CGP Atlantic cod 20K oligonucleotide microarray. Atlantic cod (Gadus morhua) received an intraperitoneal injection of either formalin-killed, atypical Aeromonas salmonicida (Asal) or PBS and transcriptional profiles of spleen tissues from Asal-injected fish were compared to those from pre-injection control fish and PBS-injected control fish. Gene expression profiles resulting from this study were compared to those from suppression subtractive hybridization library studies, that were previously performed on the same samples, and to literature. more...

Organism:

Gadus morhua

Type:

Expression profiling by array

Platform:

GPL10532

23 Samples

Download data: TXT

(Submitter supplied) The aim of this investigation was to compare transcription profiles from the ovaries and testes of mature T. thynnus to establish sex specific variations underlying their reproductive physiology.

Organism:

Thunnus thynnus

Type:

Expression profiling by array

Platform:

GPL10007

8 Samples

Download data: TXT

(Submitter supplied) We describe the design, cleaning and annotation of the gene index for sunflower and the construction of the microarray platform. In addition, we validate this tool by means of the analysis of global changes in gene expression profiles in response to water deficit as a physiological event which induces senescence, taken as a model experiment, for which reference genes have also been identified and validated (Fernandez et al., 2011). more...

Organism:

Helianthus annuus

Type:

Expression profiling by array

Platform:

GPL13610

8 Samples

Download data: TXT

(Submitter supplied) This study compares gene expression in the testis of three offshore (Pelican Shoal) and three near-shore (Tingler Island) adult male queen conchs (Strombus gigas) collected from the wild on February 15, 2007.

Organism:

Aliger gigas

Type:

Expression profiling by array

Platform:

GPL8934

6 Samples

Download data: TXT

(Submitter supplied) A holistic approach to examine differential gene expression profiles of transcripts relevant to the moulting process, across all moult cycle stages, was used in this study. Custom cDNA microarrays were constructed for P. pelagicus. The printed arrays contained 5000 transcripts derived from both the whole organism, and from individual organs such as the brain, eyestalk, mandibular organ and Y-organ from all moult cycle stages. more...

Organism:

Portunus pelagicus

Type:

Expression profiling by array

Platform:

GPL4797

30 Samples

Download data

(Submitter supplied) The copepod, Calanus finmarchicus is a keystone species for the North Atlantic. Because of recent changes in the geographic distribution of this species, there are questions as to how this organism responds physiologically to environmental cues. Molecular techniques allow for examination and new understanding of these physiological changes. Here, we describe the development of a microarray for high-throughput studies of the physiological ecology of C. more...

Organism:

Calanus finmarchicus

Type:

Expression profiling by array

Platform:

GPL14742

11 Samples

Download data: GPR

(Submitter supplied) The domestic ferret (Mustela putorius furo) has been used as animal model for decades, largely because its susceptibility to infection with a large number of pathogens such as influenza virus, SARS Corona virus and Canine distemper virus. Despite its importance for biomedical research, little is known about the genome of the M. Furo. The number of reagents for molecular and immunological analysis is thus restricted. more...

Organism:

Mustela putorius furo

Type:

Expression profiling by array

Platform:

GPL9774

24 Samples

Download data: TXT

(Submitter supplied) Background The domestic pig is an important livestock species for meat production worldwide and is becoming an established biomedical research model. As a result, there is a strong interest in the factors that affect the efficient production of viable embryos and offspring in this species using either in vivo or in vitro production methods. A limited understanding of the molecular mechanisms involved in this critical physiological process has inhibited our ability to fully elucidate these factors. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL14925

2 Samples

Download data: GPR, TXT

(Submitter supplied) 2-4-8 cell porcine embryo technical repeat dye-swap for Agilent EMPV1 (4x44k Format)

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL14925

1 Sample

Download data: GPR

(Submitter supplied) To identify the transcriptome divergence and sex roles underlying it in recently diverged Drosophia pseudoobscura group, we compare the global gene expression of 7-day-old whole body adults of pure species.

Organism:

Drosophila pseudoobscura; Drosophila pseudoobscura bogotana; Drosophila persimilis

Type:

Expression profiling by array

Platform:

GPL8884

18 Samples

Download data: GPR

(Submitter supplied) This paper describe the whole genome-wide analysis of differences in postnatal intestinal from 0 day to 21 days of piglets. Using Genome Array，We generated transcriptase profiles of intestine from the Landrace piglets at 0, 3, 8, 14，21 days post natal (dpn). We identified 4014 differentially expressed transcripts during intestinal developing in Landrace piglets. Respectively, the Gene Ontology processes and the time sequence profile analysis of differentially expressed genes were constructed. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3533

5 Samples

Download data: CEL, CHP