GEO DataSets

(Submitter supplied) Transcriptional profiling comparing the genes upregulated or downregulated by active hGR-Xebf3 by treating hormone DEX to those by non-active hGR-Xebf3 in Xenopus animal caps.

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platforms:

GPL11258 GPL10302

8 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling comparing the genes upregulated or downregulated by active hGR-Xebf3 by treating hormone DEX to those by non-active hGR-Xebf3 in Xenopus animal caps.

Organism:

Xenopus laevis

Type:

Expression profiling by array

Platform:

GPL10302

2 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17021

22 Samples

Download data: CSV

(Submitter supplied) In this work we compare the molecular functions of Myf5 and MyoD, two highly related bHLH transcription factors that regulate skeletal muscle specification and differentiation. We find MyoD and Myf5 bind the same sites genome-wide but have distinct functions: Myf5 induces histone acetylation without Pol II recruitment or robust gene activation, whereas MyoD induces histone acetylation, recruits PolII and robustly activates gene transcription.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

12 Samples

Download data: CSV

(Submitter supplied) In this work we compare the molecular functions of Myf5 and MyoD, two highly related bHLH transcription factors that regulate skeletal muscle specification and differentiation. We find MyoD and Myf5 bind the same sites genome-wide but have distinct functions: Myf5 induces histone acetylation without Pol II recruitment or robust gene activation, whereas MyoD induces histone acetylation, recruits PolII and robustly activates gene transcription.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17021

10 Samples

Download data: CSV

(Submitter supplied) Gene expression changes induced by MyoD or Myf5 were examined in a double-knockout fibroblast cell line lacking endogenous functional myoD or myf5 genes. Use of this cell line precluded the possibility of auto- or cross-activation of endogenous myoD or myf5. Myogenin or hrGFP were expressed in parallel samples as controls. Following infection with retrovirus - expressing the relevant myogenic regulatory factor (MRF) from the viral LTR promoter and hrGFP through an IRES element in the same mRNA transcript - GFP+ cells were sorted by FACS and harvested for total RNA. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

12 Samples

Download data: CEL, EXP

(Submitter supplied) Muscle satellite cells are a self-renewing pool of stem cells that give rise to daughter myogenic precursor cells in adult skeletal muscle. Published and preliminary data indicated that MyoD and p53 genes are involved in satellite cell differentiation. We would like to know what downstream genes of both transcription factors are affected in satellite cell-derived myoblasts (MyoD-/-, p53 -/-). Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL82 GPL83 GPL81

25 Samples

Download data: CEL, EXP

(Submitter supplied) Identification of Tbx6 genomic binding sites during zebrafish somitogenesis in order to identify Tbx6 targets genes

Organism:

Danio rerio

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL16509

4 Samples

Download data: TXT

(Submitter supplied) Pbx homeodomain proteins have been implicated in the regulation of gene expression during muscle development. Whether Pbx proteins are required broadly for the regulation of muscle gene expression or are required for the expression of a specific subset of muscle gene expression is not known. We employed microarrays to determine the requirements for Pbx proteins during zebrafish development. Keywords: developmental time course analysis

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL1319

12 Samples

Download data: CEL

(Submitter supplied) The developing vertebrate embryo is exquisitely sensitive to retinoic acid (RA) concentration, particularly during anteroposterior patterning. In contrast to Nodal and Wnt signaling, RA was not previously considered to be an instructive signal in mesoderm formation during gastrulation. Here we show that RARγ is indispensable for the expression of early mesoderm markers and is, therefore, an obligatory factor in mesodermal competence and/or maintenance. more...

Organism:

Xenopus laevis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25501

24 Samples

Download data: XLSX

(Submitter supplied) Ebf genes regulate differentiation of several cell type. Ebf2 is expressed in Schwann cells and Ebf2-/- mice show among other phenotypical abnormalities a delay in the onset of myelination associated to a decreased expression of genes regulating myelination. In addition at one month of age Ebf2-/- mice show decreased motor conduction velocity and morphological alteration in sciatic nerves. Ebf2 target genes are unknown. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

5 Samples

Download data: CEL

(Submitter supplied) NIH-3T3 cells transduced with either EBF1-, PPARg2- or empty vector were stimulated with hormones to initiate adipocyte differentiation. RNA extraction was done using TriZol at d0, d2, d4 and d10 after stimulation. Samples were handled according to standard affymetrix protocols. Keywords = Adipogenesis Keywords = adipocyte Keywords = early B-cell factor 1 (EBF1) Keywords = commitment Keywords = differentiation Keywords = NIH-3T3 Keywords = pparg Keywords: time-course

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1298

Platform:

GPL81

53 Samples

Download data: CEL, EXP

Analysis of NIH-3T3 embryonic fibroblasts induced to differentiate into adipocytes by overexpression of early B cell factor (EBF)-1 or peroxisome proliferator-activated receptor (PPAR)gamma2 transcription factors. Cells examined at various time points up to 10 days following overexpression.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 agent, 4 time sets

Platform:

GPL81

Series:

GSE2192

53 Samples

Download data: CEL, EXP

(Submitter supplied) We have determined that sustained expression of EBF suppresses alternate lineage genes independently of Pax5. Keywords: Transcription factor EBF restricts alternate lineage options and promotes B cell fate commitment independently of Pax5.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL

(Submitter supplied) The mammary gland develops mainly postnatally, when during pregnancy the epithelium grows out into the mammary fat pad and forms a network of epithelial ducts. During pregnancy, these ducts branch and bud to form alveoli. These alveoli produce the milk during lactation. After 7 days of lactation, involution was induced by force weaning the pups. The newly formed epithelium undergoes apoptosis and is removed from the tissue by neighbouring epithelial cells. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL81

54 Samples

Download data: CEL

(Submitter supplied) The homeobox genes of the orthodenticle (otd)/Otx family play conserved roles in embryogenesis of the head and brain. Gene replacement experiments show that the Drosophila otd gene and orthologous mammalian Otx genes are functionally equivalent, in that overexpression of either gene in null mutants of Drosophila or mouse can restore defects in cephalic and brain development. This suggests that otd and Otx genes can control a comparable subset of downstream target genes in either organism. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Dataset:

GDS23

Platform:

GPL70

16 Samples

Download data

Analysis of the effects of overexpression of orthodenticle homeobox genes otd (Drosophila) and Otx2 (human) reveals evolutionary conservation. The otd/Otx2 homeobox genes play a role in embryogenesis of the head and brain.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, count, 2 shock, 3 strain sets

Platform:

GPL70

Series:

GSE32

16 Samples

Download data

(Submitter supplied) Fibroblasts from PRDM12 patients and unaffected wildtype relatives were cultured until near confluency. The transcriptional profile of those cells was determined by mRNA sequencing and uncovered differential expression in several known pain and neurodevelopmental genes.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

4 Samples

Download data: TXT

(Submitter supplied) BACKGROUND: When resources are scant, C. elegans larvae arrest as long-lived dauers under the control of insulin/IGF- and TGFbeta-related signaling pathways. : We have identified genes that show different levels of expression in a comparison of wild-type L2 or L3 larvae (non-dauer) to TGFbeta mutants at similar developmental stages undergoing dauer formation. Many insulin/IGF pathway and other known dauer regulatory genes have changes in expression that suggest strong positive feedback by the TGFbeta pathway. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

4 related Platforms

10 Samples

Download data

(Submitter supplied) To study the early gene expression events involved in neurogenesis, we expressed the bHLH neural determination factor neurogenin 1 in mouse embryonic stem cells. We used microarray to examine the program of gene expression at 24, 48, 72 hours of transgene expression.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL, CHP