GEO DataSets

(Submitter supplied) Background: The mosquito Anopheles gambiae is a major vector of human malaria. Increasing evidence indicates that blood cells (hemocytes) comprise an essential arm of the mosquito innate immune response against both bacteria and malaria parasites. To further characterize the role of hemocytes in mosquito immunity, we undertook the first genome-wide transcriptomic analyses of adult female An. gambiae hemocytes following infection by two species of bacteria and a malaria parasite. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by array

Platforms:

GPL8551 GPL8552 GPL8550

11 Samples

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(Submitter supplied) Insect hemocytes mediate important cellular immune responses including phagocytosis and encapsulation, and also secrete immune factors such as opsonins, melanization factors, and antimicrobial peptides. In Anopheles, they contribute to the defense against malaria parasite invasion during the early sporogonic cycle. We used microarrays to identify transcripts that are specific or enriched in circulating hemocytes compared to either neuronal or to the rest of the body.

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL1321

16 Samples

Download data: CEL, CHP

(Submitter supplied) Insect hemocytes mediate important cellular immune responses including phagocytosis and encapsulation, and also secrete immune factors such as opsonins, melanization factors, and antimicrobial peptides. In Anopheles, they contribute to the defense against malaria parasite invasion during the early sporogonic cycle. We used microarrays to identify if and to what degree circulating hemocytes have altered global expression profiles after infection with the rodent malaria parasite, Plasmodium berghei

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL1321

6 Samples

Download data: CEL, CHP

(Submitter supplied) The mosquito Anopheles gambiae uses its innate immune system to control bacterial and Plasmodium infection of its midgut tissue. The activation of potent IMD pathway-mediated anti-Plasmodium falciparum defenses is dependent on the presence of the midgut microbiota, which activate this defense system upon parasite infection through a peptidoglycan recognition protein, PGRPLC. We employed transcriptomic and reverse genetic analyses to compare the P. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL17550

4 Samples

Download data: TXT, XLSX

(Submitter supplied) Transmission of malaria is dependent on the successful completion of the Plasmodium lifecycle in the Anopheles vector. Major obstacles are encountered in the midgut tissue, where most parasites are killed by the mosquito’s immune system. In the present study, DNA microarray analyses have been used to compare Anopheles gambiae responses to invasion of the midgut epithelium by the ookinete stage of the human pathogen Plasmodium falciparum and the rodent experimental model pathogen P. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL5001

24 Samples

Download data

(Submitter supplied) To examine the effects of 20E treatment on mosquito gene expression, treated mosquito cells were examined by RNA-seq to determine the influence of 20E treatment.

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25232

8 Samples

Download data: TXT

(Submitter supplied) We provide a broad overview of sequence diversity in An. gambiae mature microRNAs, including annotation of novel microRNAs identified in this study.

Organism:

Anopheles gambiae

Type:

Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL17644 GPL15693

8 Samples

Download data: TXT

(Submitter supplied) Analysis of Culex quinquefasciatus responses to West Nile virus (WNV) infection at 7 and 14 days after ingestion of infected blood in the gut and carcass tissues.

Organism:

Culex quinquefasciatus

Type:

Expression profiling by array

Platform:

GPL10712

16 Samples

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(Submitter supplied) Malaria is caused by Plasmodium parasites, which are transmitted via the bites of infected Anopheline mosquitoes. Midgut invasion is a major bottleneck for Plasmodium development inside the mosquito vectors as a rapidly responding immune system recognizes ookinetes and recruits killing factors from the midgut and surrounding tissues, dramatically reducing the population of invading ookinetes before they can successfully traverse the midgut epithelium. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15693

18 Samples

Download data: TXT

(Submitter supplied) To test the contributions of phagocytic immune cells to mosquito immunity, we depleted mosquito phagocytes using clodronate liposomes and then challenged mosquitoes with malaria parasites. Observing a large increase in infection intensity, the purpose of the RNA-seq experiment is to determine their effects of phagocyte depletion on mosquito immunity.

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25232

8 Samples

Download data: TXT

(Submitter supplied) Anopheles aquasalis transcriptome in response to Plasmodium vivax invasion of midgut

Organism:

Anopheles aquasalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26036

9 Samples

Download data: FASTA, TXT

(Submitter supplied) Malaria is as one of the most debilitating mosquito-borne global health burdens. While much of the malaria and mosquito-borne disease attention have focused on Africa, South East Asia accounts for a sizable portion of the malaria global burden. Moreover, about 50% of the Asian malaria incidence and deaths have been from India. The completion of genome sequence of Anopheles stephensi, a major malaria vector in Asia, offers new opportunities for global health innovation, not to mention for progress in deciphering the vectorial ability of this mosquito species at a molecular level. more...

Organism:

Anopheles stephensi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23543

4 Samples

Download data: GTF, TXT

(Submitter supplied) Wolbachia, an endosymbiotic bacterium, is being investigated as a vector control agent in several insect species. Along with the well known classical reproductive parasitism Wolbachia employs against its host to spread within the population, it is emerging that the bacteria can protect the host against pathogens and reduced pathogen transmission. Anopheles mosquitoes, which transmit malaria, have never been found to harbour Wolbachia in nature, and despite numerous transinfection attempts, no stable line has been developed. more...

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL1321

9 Samples

Download data: CEL

(Submitter supplied) Anopheles gambiae, the primary African malarial mosquito, exhibits numerous behaviors that are under diel and circadian control, including locomotor activity, swarming, mating, host seeking, eclosion, egg laying and sugar feeding. However, little has been performed to elucidate the molecular basis for these daily rhythms. To study how gene expression is globally regulated by diel and circadian mechanisms, we have undertaken a DNA microarray analysis of A. more...

Organism:

Anopheles gambiae; Plasmodium falciparum

Type:

Expression profiling by array

Dataset:

GDS4261

Platform:

GPL1321

104 Samples

Download data: CEL

Analysis of heads and bodies of mated, non-blood-fed, adult females maintained under a 12hr:12hr light:dark (LD) cycle or constant dark (DD) conditions; samples were collected every 4hr for 48 hr. Results provide insight into molecular mechanisms under circadian and LD cycle control.

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array, transformed count, 2 growth protocol, 26 time, 2 tissue sets

Platform:

GPL1321

Series:

GSE22585

104 Samples

Download data: CEL

(Submitter supplied) Senescence is a biological phenomenon experienced by all living eukaryote organisms. Genome-wide gene expression associated with aging has been explored in model organisms such as Drosophila melanogaster and Caenorhabditis elegans, but this has not been well understood in African malaria vector, Anopheles gambiae. Gene expression profiling using DNA microarray allows for simultaneous study of changes in mRNA levels for thousands of genes. more...

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL1321

31 Samples

Download data: CEL

(Submitter supplied) Background. The mosquito, Anopheles gambiae, is the primary vector of human malaria, a disease responsible for millions of deaths each year. To improve strategies for controlling transmission of the causative parasite, Plasmodium falciparum, we require a thorough understanding of the developmental mechanisms, physiological processes and evolutionary pressures affecting life-history traits in the mosquito. more...

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL1321

60 Samples

Download data: CEL

(Submitter supplied) Anopheles gambiae mosquitoes play an important role in malaria transmission. In sub-Saharan Africa, the dry season can last several months. The mechanisms for mosquito population to survive through the dry season are poorly understood. One possible mechanism is that adults increase their desiccation tolerance over the dry season. Genetic analyses have shown that inversions 2La, 2Rb, 2Rc, 2Rd and 2Ru are associated with aridity resistance, however little is known about the transcriptional response of genes in response to desiccation. more...

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Dataset:

GDS4552

Platform:

GPL1321

19 Samples

Download data: CEL

Analysis of adult females exposed to 30% (desiccated) relative humidity for up to 36 hrs. The An. gambiae malaria vector populations are observed year round but their densities are low during the dry season. Results provide insight into molecular basis of the malaria vector's desiccation response.

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array, count, 3 stress, 3 time sets

Platform:

GPL1321

Series:

GSE25433

19 Samples

Download data: CEL

(Submitter supplied) Background: Infection by the human malaria parasite leads to important changes in mosquito phenotypic traits related to vector competence. However, we still lack a clear understanding of the underlying mechanisms and, in particular, of the epigenetic basis for these changes. We have examined genome-wide distribution maps of H3K27ac, H3K9ac, H3K9me3 and H3K4me3 by ChIP-seq and the transcriptome by RNA-seq, of midguts from Anopheles gambiae mosquitoes blood-fed uninfected and infected with natural isolates of the human malaria parasite Plasmodium falciparum in Burkina Faso. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17644

14 Samples

Download data: BW, TXT