GEO DataSets

(Submitter supplied) Differential response of bovine mammary epithelial cells to Staphylococcus aureus or Escherichia coli agonists of the innate immune system. Mastitis caused by Escherichia coli and Staphylococcus aureus is a major pathology of dairy cows. To better understand the differential response of the mammary gland to these two pathogens, we stimulated bovine mammary epithelial cells (bMEC) with either E. coli crude lipopolysaccharide (LPS) or with S. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL17231

32 Samples

Download data: TXT

(Submitter supplied) Outcome and resolution of an udder inflammation (mastitis) depends on the type of the invading pathogen. Gram-negative pathogens, such as Escherichia coli mostly trigger strong inflammation and full local activation of the immune defense eventually resulting in pathogen elimination. Staphylococcus aureus and other Gram-positive pathogens elicit only weak immune reactions possibly allowing for pathogen persistence. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL17645

49 Samples

Download data: CEL

(Submitter supplied) Establishment of an in vitro system to explore molecular mechanisms of mastitis susceptibility in cattle by comparative expression profiling of Escherichia coli and Staphylococcus aureus inoculated primary cells sampled from cows with different genetic predisposition for somatic cell score Primary bovine mammary gland epithelial cells (pbMEC) were sampled from the udder parenchyma of cows that were selected for high and low mastitis susceptibility by applying a marker assisted selection strategy considering QTL and molecular marker information of a repetitively confirmed QTL for SCS in the telomeric region on BTA18 The cells were cultivated and subsequently inoculated with heat inactivated mastitis pathogens Escherichia coli and Staphylococcus aureus, respectively. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Dataset:

GDS4437

Platform:

GPL2112

88 Samples

Download data: CEL

Mammary gland epithelial cells of cows with favorable somatic cell score (SCS) QTL allele Q and unfavorable allele q after S. aureus or E.coli exposure for up to 24 hours. SCS is an indicator of milk quality and udder health. Results provide insight into molecular basis of mastitis susceptibility.

Organism:

Bos taurus

Type:

Expression profiling by array, count, 2 genotype/variation, 3 protocol, 3 time sets

Platform:

GPL2112

Series:

GSE24560

88 Samples

Download data: CEL

(Submitter supplied) Background: Udder infections with environmental pathogens like Escherichia coli are a serious problem for the diary industry. Reduction of incidence and severity of mastitis is desirable and mild priming of the immune system either through vaccination or with low doses of an immune stimulant like lipopolysaccharide LPS was previously found to dampen detrimental effects of a subsequent infection. Monocytes / macrophages are known to develop tolerance to the endotoxin (ET) LPS as adaptation strategy to prevent exuberant inflammation. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

24 Samples

Download data: CEL

(Submitter supplied) Infections of the udder by Escherichia coli very often elicit acute inflammation, while Staphylococcus aureus infections tend to cause mild, subclinical inflammation and persistent infections. The molecular causes undercovering the different disease patterns are poorly understood. We therefore profiled kinetics and extent of global changes in the transcriptome of primary bovine mammary epithelia cells (MEC) subsequent to challenging them with heat inactivated preparations of E. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Dataset:

GDS4406

Platform:

GPL2112

30 Samples

Download data: CEL

Analysis of mammary epithelial cells (MECs) challenged with heat-killed particles of E. coli or S. aureus for up to 24 hrs. Results provide insight into the molecular mechanisms underlying the pathogen-specific disease patterns.

Organism:

Bos taurus

Type:

Expression profiling by array, transformed count, 4 stress, 5 time sets

Platform:

GPL2112

Series:

GSE25413

30 Samples

Download data: CEL

(Submitter supplied) Mastitis is an inflammation of the mammary gland (MG), usually due to bacterial infection. Although considerable attention has been paid to this economically important disease, the early stages of the host response remain poorly defined. In particular, it is unclear how mammary epithelial cells (MEC), a first barrier against pathogens, respond to infection. Indeed, it is difficult to differentiate between the contributions of MEC and infiltrating immune cells to gene expression profiles of mammary tissue during mastitis. more...

Organism:

Capra hircus; Ovis aries

Type:

Expression profiling by array

Platform:

GPL10427

20 Samples

Download data: TXT

(Submitter supplied) Mastitis in dairy cattle can result from infection by a range of microorganisms but is principally caused by coliform bacteria and gram positive bacteria such as Staphylococcus aureus (S. aureus). The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL6082

36 Samples

Download data: GPR

(Submitter supplied) Mastitis in dairy cattle can result from infection by a range of microorganisms but is principally caused by coliform bacteria and gram positive bacteria such as Staphylococcus aureus (S. aureus). The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL6082

12 Samples

Download data: GPR

(Submitter supplied) Mastitis in dairy cattle can result from infection by a range of microorganisms but is principally caused by coliform bacteria and gram positive bacteria such as Staphylococcus aureus (S. aureus). The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL6082

12 Samples

Download data: GPR

(Submitter supplied) Mastitis in dairy cattle can result from infection by a range of microorganisms but is principally caused by coliform bacteria and gram positive bacteria such as Staphylococcus aureus (S. aureus). The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL6082

12 Samples

Download data: GPR

(Submitter supplied) Mastitis remains one of the most prevalent and costly diseases impacting the dairy industry worldwide. Escherichia coli is an environmental bacterium that frequently causes intramammary infections, the outcome of which depends on the capacity of the host to recognize and clear the bacterial pathogen. E. coli intramammary infection elicits localized and systemic responses, some of which have been characterized in mammary secretory tissue. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL3301

36 Samples

Download data: TXT

(Submitter supplied) Background: Staphylococcus aureus is a major pathogen of humans and animals. Host genetics influence the susceptibility to S. aureus infections, and genes determining infection outcome remain to be identified. Here, we used outbred animals from a divergent selection on susceptibility towards Staphylococcus infection to explore host immunogenetics. Methodology/Principal Findings: We investigated in vitro how mammary epithelial cells (MEC) respond to live S. more...

Organism:

Ovis aries

Type:

Expression profiling by array

Platform:

GPL10427

118 Samples

Download data: TXT

(Submitter supplied) Mastitis is a common disease in dairy cows and brings massive losses to the dairy industry. m6A is a type of modification strongly associated with many diseases. However, the role of m6A in mastitis caused by Staphylococcus aureus and Escherichia coli has not been investigated.We used MeRIP-seq technology to sequence the bovine mammary epithelial cells (MAC-T) infected with inactivated S. aureus/E. more...

Organism:

Bos taurus

Type:

Other

Platform:

GPL23295

18 Samples

Download data: XLSX

(Submitter supplied) Escherichia coli and Staphylococcus aureus are two common pathogenic microorganisms that cause mastitis in dairy cows. They can cause clinical mastitis and subclinical mastitis. In recent studies, lncRNAs have been found to play an important role in the immune responses triggered by microbial inducers. However, the actions of lncRNAs in bovine mastitis remain unclear. The purpose of this study was to explore the lncRNA profile on mastitis.

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL26012

9 Samples

Download data: XLSX

(Submitter supplied) Mastitis in dairy cows is one of the most costly and prevalent diseases affecting dairy cows world wide. Insight in the molecular regulation of the host immune response to an E. coli infection, could help to develop new strategies to prevent cattle from E. coli infection. Here we performed a gene-expression analysis from udder tissue exposed to a controlled E. coli infection at T=24h post infection (p.i.) representing the acute phase response and at T=192h p.i. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

49 Samples

Download data: CEL

(Submitter supplied) The benefit of treatment in mild to moderate cases of E. coli mastitis in dairy cows remains a topic of discussion. We investigated the effect of intramammary treatment with Cefapirin + Prednisolone compared to Cefapirin only on gene expression profiles in experimentally induced E. coli mastitis.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

20 Samples

Download data: CEL

(Submitter supplied) Bovine mastitis, an inflammatory reaction of the mammary gland, is commonly caused by Staphylococcus aureus or Escherichia coli infections in high-yielding dairy cows. Immunotranscriptomic responses of bovine mammary epithelial cell (bMEC) are pivotal for the understanding of the defense against invading pathogens, and for the fate of udder inflammation. The intracellular chemotaxic protein Cyclophilin A (CyPA), secreted by bMEC during inflammatory reaction is being considered as a marker for the early diagnosis of mastitis. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11648

4 Samples

Download data: TXT

(Submitter supplied) Purpose: The aim of the study was to compare the miRNA expression in non-infected (H) mammary gland parenchyma samples with that of glands infected with coagulase-positive staphy lococci (CoPS) or coagulase-negative staphylococci (CoNS) using next-generation sequencing. Methods: miRNA-seq analysis was performed on mammary gland parenchyma samples collected from non-infected cows and those infected with coagulase-positive or -negative staphylococci. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL17875

36 Samples

Download data: FA