GEO DataSets

(Submitter supplied) Metabolic stress associated with negative energy balance in high producing dairy cattle and obesity in women is a risk factor for decreased fertility. Non-esterified fatty acids (NEFA) are involved in this pathogenesis as they jeopardize oocyte and embryo development. Growing evidence indicates that maternal metabolic disorders can disturb epigenetic programming, and more specifically DNA methylation, in the offspring. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

4 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bos taurus

Type:

Expression profiling by array; Methylation profiling by genome tiling array

Platforms:

GPL13226 GPL18384

12 Samples

Download data: TXT

(Submitter supplied) Metabolic stress associated with negative energy balance in high producing dairy cattle and obesity in women is a risk factor for decreased fertility. Non-esterified fatty acids (NEFA) are involved in this pathogenesis as they jeopardize oocyte and embryo development. Growing evidence indicates that maternal metabolic disorders can disturb epigenetic programming, and more specifically DNA methylation, in the offspring. more...

Organism:

Bos taurus

Type:

Methylation profiling by genome tiling array

Platform:

GPL18384

8 Samples

Download data: TXT

(Submitter supplied) Objective: To study post-hatching development after transfer of morphologically good quality embryos that survived in vitro maturation under lipolytic conditions using a well-established and recognized bovine model. Design: prospective study with a crossover design. Setting: research laboratory. Animals: 8 healthy multiparous non-lactating Holstein cows used in repeated embryo transfers. Intervention(s): Bovine cumulus oocyte complexes (COCs) were in vitro matured in the presence of either 1) a combination of elevated pathophysiological concentration of palmitic acid (PA) and physiological concentrations of stearic acid (SA) and oleic acid (OA) (HIGH PA), or 2) physiological concentrations of PA, SA and OA (BASAL). more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22316

53 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Sus scrofa

Type:

Expression profiling by array; Methylation profiling by array

Platforms:

GPL28869 GPL28870

8 Samples

Download data: TXT

(Submitter supplied) There is an inevitable need for combining in vitro and in vivo culture systems during specific developmental time points to facilitate a comprehensive understanding of early embryo development which would yield insights into the molecular pathways controlling early development and to improve our knowledge in regulation of embryonic development. In current study, we aimed to understand the influences of in vitro culture conditions on bovine blastocyst originated in vivo at 2- or 8-cell stagre. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

12 Samples

Download data: TXT

(Submitter supplied) The culture conditions of the early preimplantation embryos affects the DNA methylation landscape of the resulting blastocysts.

Organism:

Bos taurus

Type:

Methylation profiling by genome tiling array

Platform:

GPL18384

16 Samples

Download data: TXT

(Submitter supplied) Gametic DNA methylation is essential to the viability of the embryo and participates in developmental programming. Here, we performed a comparative epigenomic analysis of DNA methylation in spermatozoa from humans, mice, rats and mini-pigs to get insight into species specificity of the gametic epigenetic blueprint. Using data from whole-genome bisulfite sequencing (WGBS) and reduced representation bisulfite sequencing (RRBS), we compared the methylation profiles of orthologous single-CpG sites. more...

Organism:

Sus scrofa

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL26351

4 Samples

Download data: COV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bos taurus

Type:

Methylation profiling by genome tiling array

Platform:

GPL18384

8 Samples

Download data: TXT

(Submitter supplied) The aim was to study the potential effect of hyperinsulinemia on gene expression and differential DNA-methylation of cow embryos by adding 0.1 µg/ml (INS0.1) during in vitro oocyte maturation.

Organism:

Bos taurus

Type:

Methylation profiling by genome tiling array

Platform:

GPL18384

4 Samples

Download data: TXT

(Submitter supplied) The aim was to study the potential effect of hyperinsulinemia on gene expression and differential DNA-methylation of cow embryos by adding 10 µg/ml (INS10) during in vitro oocyte maturation.

Organism:

Bos taurus

Type:

Methylation profiling by genome tiling array

Platform:

GPL18384

4 Samples

Download data: TXT

(Submitter supplied) we induced an energetic stress on in vitro embryos to observe the epigenetic response associated with metabolic stress. Embryos were created with slaughterhouse cows oocytes and then exposed to an elevated glucose concentration (5mM vs 0.2mM for the control embryos) for the first 3 days post fecundation and then cultivated in normal media until blastocyst stage. Blastocysts were collected from 4 different weeks of culture. more...

Organism:

Bos taurus

Type:

Methylation profiling by array

Platform:

GPL18384

4 Samples

Download data: TXT

(Submitter supplied) IVP blastocysts exposed to hyperglycaemia during early phase of development, i.e from zygote to 8-16 cells stages. Genes expression analysis is done at day 7.5 (7 days of embryo culture).

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

8 Samples

Download data: TXT

(Submitter supplied) Background: With the rapid development of assisted reproductive technology (ART), although ovulation induction has made great progress, the accompanying health risks should not be ignored. Epigenetic modifications play an important role during the development of gametes and embryos. Recently, the epigenetic abnormalities caused by superovulation have attracted increasing attention. The follicle-stimulating hormone (FSH) and human menopausal gonadotropin (hMG) are common gonadotropins used for superovulation and appropriate concentration of them might be necessary. more...

Organism:

Mus musculus

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL13112

40 Samples

Download data: BW

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing; Methylation profiling by array

Platforms:

GPL28869 GPL28870

8 Samples

Download data: TXT

(Submitter supplied) The transcriptome pattern in blastocyst that developed from oocytes exposed to the high level of NEFAs with cocultured of monolayer of GSs was investigated. Genome-wide transcriptome analysis was performed by micro array using EDMA platform.

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL28870

4 Samples

Download data: TXT

(Submitter supplied) The DNA methylation pattern in blastocyst that developed from oocytes exposed to the high level of NEFAs was investigated. Genome-wide DNA methylation analysis was performed by micro array using EDMA platform.

Organism:

Sus scrofa

Type:

Methylation profiling by array

Platform:

GPL28869

4 Samples

Download data: TXT

(Submitter supplied) Microarray analysis was performed in ooplast samples to identify gene expression profiles associated with the embryo capacity to develop to blastocyst stage

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13515

32 Samples

Download data: GPR

(Submitter supplied) Comparison of mitochondrial DNA (mtDNA) methylation patterns in oocytes, blastocysts and ovarian granulosa cells indicates hitherto unsuspected dynamics. Oocytes and blastocysts recovered from animals subjected to ovarian stimulation and from abattoir ovaries were analyzed using bisulphite transformation of DNA followed by whole genome sequencing. The number of mtDNA copies is quite high in oocytes (200–400,000) and early embryos, resulting in very high coverage (> 3000 X) and very low p values for each of 716 cytosine-based nucleosides. more...

Organism:

Bos taurus

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL23295

6 Samples

Download data: TXT

(Submitter supplied) Pig oocytes were stained with Brilliant cresyl blue to identify mtDNA deficient oocytes.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24486

40 Samples

Download data: TXT