GEO DataSets

(Submitter supplied) The objective was to evaluate the effect of nutrient restriction and intramammary lipopolysaccharide (LPS) challenge on mammary gland (MG) gene expression in early lactation cows. At 24 ± 3 d in milk, multiparous cows were either allowed to continue ad libitum intake of a lactation diet (CON, n = 6), or the ration was diluted with barley straw (48% DM) for 4 d (RES, n = 6). On d 3, one healthy rear mammary quarter was infused with 50 µg of LPS. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11648

12 Samples

Download data: TXT

(Submitter supplied) Background Our objective was to compare mammary tissue gene expression profiles during a Streptococcus uberis (S. uberis) mastitis challenge between lactating cows subjected to dietary-induced negative energy balance (NEB; n = 5) and cows fed ad libitum to maintain positive energy balance (PEB; n = 5). The NEB cows were feed-restricted to 60% of calculated net energy for lactation requirements for 7 d, and cows assigned to PEB were fed the same diet for ad libitum intake. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL8776

40 Samples

Download data: TXT

(Submitter supplied) The effects of a negative energy balance induced by a nutritional restriction on mammary gland were evaluated using the 4x44K bovine microarray from Agilent

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11648

12 Samples

Download data: TXT

(Submitter supplied) Effect of feed restriction in mid lactation Holstein (H) and Montbéliarde (M) cows on mammary glande miRNA profil.

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL23295

24 Samples

Download data: TXT

(Submitter supplied) Data from one animal from B9_B12 group (Cow 31) were not taken into account for the liver samples Treated animals were compared to control (non supplemented cows)

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11648

25 Samples

Download data: TXT

(Submitter supplied) Cows were fed a lactation diet at ad libitum intake (n = 6). At 27±3 days in milk, cows were injected with 50 µg of LPS E. coli in one healthy rear mammary quarter. Milk samples were collected just before LPS challenge (LPS-) and 6.5 h after LPS challenge (LPS+) from the same cows. Microarray analysis was performed using customized 8x60K ruminant miRNA microarrays to compare LPS- to LPS+ miRNome. MiRNome comparison between LPS- and LPS+ identified 37 differentially abundant miRNAs (q-value ≤ 0.05)

Organism:

Capra hircus; Bos taurus; Ovis aries

Type:

Non-coding RNA profiling by array

Platform:

GPL33332

12 Samples

Download data: TXT

(Submitter supplied) Transcript profiling was performed by Affymetrix microarray analysis and SAGE to characterize changes in gene expression in the bovine mammary gland in response to 4× versus 2× daily milking during the first week of lactation. These changes in gene expression may contribute to the increased milk production observed in response to increased milking frequency. Our results indicate that increased milking frequency alters expression of genes in the mammary gland related to increased extracellular matrix remodeling, neovascularization, metabolism, cell proliferation and apoptosis. more...

Organism:

Bos taurus

Type:

Expression profiling by array; Expression profiling by SAGE

Platforms:

GPL2112 GPL223

9 Samples

Download data: CEL

(Submitter supplied) Effect of breed in mid lactation Holstein (H) and Montbéliarde (M) cows on mammary glande miRNA profile. Genetic polymorphisms are known to influence milk production and composition. However, genomic mechanisms involved in the genetic regulation of milk component synthesis are not completely understood. MicroRNAs (miRNA) regulate gene expression. The objective of the present study was to compare mammary gland miRNomes of two dairy cow breeds, Holstein and Montbéliarde, with different dairy performances. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL23295

11 Samples

Download data: TXT

(Submitter supplied) The ruminant liver has multiple roles in the dairy cow and many of these are crucial in nutrient supply during lactation. Reduced feed intake alters the expression of many genes and pathways in the liver, inducing a period of negative energy balance. Once-daily milking is a management strategy to reduce the effects of periods of negative energy balance so the objective of this study was to determine if once-daily milking altered hepatic gene transcription during a period of negative energy balance induce by caloric restriction. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL9712

45 Samples

Download data: TXT

(Submitter supplied) Cows exposed to short day photoperiod (SD, 8L:16D) during the 60-day non-lactating period prior to parturition produce more milk in their subsequent lactation compared to cows exposed to long day photoperiod (LD,16L:8D). Although this response is well-established in dairy cows, the underlying mechanisms are not understood. We hypothesized that differential gene expression in cows exposed to SD or LD photoperiods during the dry period could be used to identify the functional basis for the subsequent increase in milk production during lactation. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

14 Samples

Download data: CEL

(Submitter supplied) Milking dairy cows four times daily (4X) instead of twice daily (2X) during early lactation stimulates an increase in milk yield that partly persists through late lactation; however, the mechanisms behind this response are unknown. We hypothesized that the acute mammary response to regular milkings would be transient and would involve different genes from those that may be specifically regulated in response to 4X. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Dataset:

GDS4009

Platform:

GPL2112

14 Samples

Download data: CEL

Analysis of mammary biopsies from lactating cows assigned to unilateral frequent milking (2X daily of left udder half, 4X daily of right udder half). Biopsies obtained at 5 days in milk, immediately after the 4X udder half had been milked, or 2.5 h after both udder halves had last been milked.

Organism:

Bos taurus

Type:

Expression profiling by array, transformed count, 2 other, 2 protocol, 7 specimen, 3 time sets

Platform:

GPL2112

Series:

GSE27380

14 Samples

Download data: CEL

(Submitter supplied) BACKGROUND: The lactating mammary gland responds to changes in milking frequency by modulating milk production. This response is locally regulated and, in dairy cows, the udder is particularly sensitive during early lactation. Relative to cows milked twice-daily throughout lactation, those milked four-times-daily for just the first 3 weeks of lactation produce more milk throughout that lactation. We hypothesized that the milk yield response would be associated with increased mammary cell turnover and changes in gene expression during frequent milking and persisting thereafter. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

36 Samples

Download data: CEL

(Submitter supplied) Feed restriction and L-carnitine infusion are known to affect the liver metabolism of dairy cows. In the present experiment the effects on liver transcriptome of feed restriction and L-carnitine abomasal infusion and the interaction of the two in mid-lactation Holstein dairy cows was assessed. Data clearly indicated a lack of transcriptomics effect by L-carnitine but a strong effect due to feed restriction. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL16454

19 Samples

Download data: GPR

(Submitter supplied) The objective of this study was to analyze the mammary gland transcriptome to determine how preweaning nutrient supply alters the molecular mechanisms that regulate preweaning mammary development. Holstein heifers were fed via milk replacer either an elevated level of nutrient intake (ELE; on average, 5.9 ± 0.2 Mcal ME in 8.4 L of milk replacer (MR)/d, n = 6) or a restricted amount of nutrients (RES; 2.8 ± 0.2 Mcal ME in 4L MR/day, n = 5) for 54 d postnatal, at which point they were slaughtered and samples of mammary parenchyma tissue were obtained. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL20768

11 Samples

Download data: CEL

(Submitter supplied) Liver and mammary gland are among the most prominent organs during lactation in dairy cows. With the purpose to understand 1) the adaptation of liver and mammary to different levels of forage/concentrate ration (60:40 vs. 40:60) and 2) the crosstalk between the two organs during lactation, a transcriptome analysis was performed on liver and mammary tissues of 10 primiparous dairy cows in mid-lactation. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11649

20 Samples

Download data: TXT

(Submitter supplied) There is a lack of systematic investigations of large-scale transcriptome patterns associated with normal breast development. Herein, we profiled whole-transcriptome (by microarrays) of normal mammary glands in female Sprague-Dawley rats, an animal model widely used in breast cancer research, across six distinctive developmental stages – pre-pubertal, peri-pubertal, pubertal, lactation, and adult parous and age-matched nulliparous.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL17117

33 Samples

Download data: CEL