GEO DataSets

(Submitter supplied) Optimal health requires perpetual transcriptional fidelity of gene expression. SKN-1/NRF2 is a cytoprotective transcription factor in C. elegans that regulates the expression of cellular defenses during stress, including: nutrient deprivation, redox imbalance, and xenobiotic and pathogen exposure. Constitutive activation of SKN-1 results in pleiotropic outcomes, including shortened lifespan and protective redistribution of somatic fat to the germline. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13657

15 Samples

Download data: TXT

(Submitter supplied) The Caenorhabditis elegans oxidative stress response transcription factor, SKN-1, is essential for the maintenance of redox homeostasis and is a functional ortholog of the Nrf family of transcription factors. The numerous levels of regulation that govern these transcription factors underscore their importance. Here, we add a thioredoxin, encoded by trx-1, to the expansive list of SKN-1 regulators. We report that loss of trx-1 promotes nuclear localization of intestinal SKN-1 in a redox-independent, cell non-autonomous fashion from the ASJ neurons. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13657

4 Samples

Download data: XLS

(Submitter supplied) In C. elegans, ablation of germline stem cells (GSCs) extends lifespan, but also increases fat accumulation and alters lipid metabolism, raising the intriguing question of how these effects might be related. Here we show that a lack of GSCs results in a broad transcriptional reprogramming, in which the conserved detoxification regulator SKN-1/Nrf increases stress resistance, proteasome activity, and longevity. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13657

12 Samples

Download data: CSV

(Submitter supplied) The nematode Caenorhabditis elegans offers currently untapped potential for carrying out high-throughput, live-animal screens of low molecular weight compound libraries to identify molecules that target a variety of cellular processes. We previously used a bacterial infection assay in C. elegans to identify 119 compounds that affect host-microbe interactions among 37,214 tested. We subsequently found that one of these small molecules, RPW-24, protects C. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Dataset:

GDS4243

Platform:

GPL200

6 Samples

Download data: CEL

Analysis of bacterial infected C. elegans treated with 70 μM RPW-24 or DMSO. In a C.elegans-bacterial infection assay screening small molecules, RPW-24 was identified as a putative antimicrobial agent. Results provide insight into C. elegans conserved immune response to bacterial infection.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL200

Series:

GSE37266

6 Samples

Download data: CEL

(Submitter supplied) Transcriptional profiling of adult C.elegans exposed to E.coli or to GFP-expressing P. aeruginosa (strain PA14). For P. aeruginosa exposure, worms were separated into 2 groups - fully colonized (green) or non-colonized (dark).

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL9458

14 Samples

Download data: GPR

(Submitter supplied) Muscleblind-like splicing regulators (MBNLs) are RNA-binding factors that have an important role in developmental processes. Dysfunction of these factors is a key contributor of different neuromuscular degenerative disorders, including Myotonic Dystrophy type 1 (DM1). Since DM1 is a multisystemic disease characterized by symptoms resembling accelerated aging, we asked which cellular processes do MBNLs regulate that make them necessary for normal lifespan. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19757

6 Samples

Download data: TXT

(Submitter supplied) We used RNA-seq to identify gene expression changes in C. elegans ddi-1(icb156) mutants

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22765

6 Samples

Download data: H5

(Submitter supplied) Oxidative stress may play a role in normal aging. SKN-1 is a transcription factor necessary for intestine development in Caenorhabditis elegans, which also regulates the response to oxidative stress post-embryonically. Using DNA microarrays, we found that oxidative stress induces the antioxidant response, the heat shock response, and detoxification genes, while the expression of genes involved in homeostasis, development, and reproduction were decreased. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL200

11 Samples

Download data: CEL

(Submitter supplied) Synchronized C. elegans cultures of three geontypes -- wildype N2, daf-2(e1370) and sma-6(wk7) -- were prepared using standard techniques (http://cmgm.stanford.edu/~kimlab/index_methods.html). Live young adult worms were split between NG plates pre-seeded with the non-pathogenic E. coli strain OP50 or the Pseudomonas aeruginosa clinical isolate PA14 and incubated at 25C for 4 or 24 hours before harvesting. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

4 related Platforms

26 Samples

Download data: TXT

(Submitter supplied) Analysis of genes differentially expressed between daf-2(e1368) and daf-2(e1368);pmk-1(km25) and between daf-2(e1368) and daf-2(e1368);daf-16(mgDf47). These studies identified genes upregulated by wild-type PMK-1 and wild-type DAF-16. Keywords: genetic modification

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Dataset:

GDS3253

Platform:

GPL200

9 Samples

Download data: CEL

(Submitter supplied) Analysis of differential gene expression in C. elegans adults exposed to three different bacteria: E. coli strain OP50, wild-type P. aeruginosa PA14 and gacA mutant PA14. Samples were analyzed at 4 hours and 8 hours after exposure to the different bacteria. These studies identified C. elegans genes induced by pathogen infection. Keywords: Time course, response to pathogen infection

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Dataset:

GDS3252

Platform:

GPL200

18 Samples

Download data: CEL

Analysis of daf-2(e1368) loss-of-function mutant and daf-2(e1368);pmk-1(km25) and daf-2(e1368);daf-16(mgDf47) double mutants. PMK-1 and DAF-16 confer enhanced pathogen resistance on daf-2 mutants relative to wild-type. Results suggest DAF-16 and PMK-1 upregulate distinct genes to promote immunity.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, count, 3 genotype/variation sets

Platform:

GPL200

Series:

GSE5801

9 Samples

Download data: CEL

Analysis of strain fer-15(b26);fem-1(hc17) young adults exposed for up to 8 hrs to pathogenic Pseudomonas aeruginosa strain PA14, less pathogenic PA14 mutant gacA, and E. coli strain OP50 (normal food source). Results provide insight into the molecular basis of the response to a bacterial pathogen.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array, count, 3 infection, 2 time sets

Platform:

GPL200

Series:

GSE5793

18 Samples

Download data: CEL

(Submitter supplied) Many pathogens secrete toxins that target key host processes resulting in the activation of immune pathways. The secreted Pseudomonas aeruginosa toxin Exotoxin A (ToxA) disrupts intestinal protein synthesis which triggers the induction of a subset of P. aeruginosa-response genes in the nematode Caenorhabditis elegans. We found that losing one ToxA-induced C. elegans gene, the Tribbles pseudokinase ortholog nipi-3, results in hypersusceptibility to both P. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL200

9 Samples

Download data: CEL

(Submitter supplied) Excess iodide could lead to larve arrest to C. elegans, we screened for mutants that can survive in excess iodide and identified gain-of-function mutations in skn-1 and loss-of-function mutations in wdr-23 and bli-3. Genetic study uncover that wdr-23(lf) can interact with bli-3 mutations in a manner different from skn-1(gf). Transcriptome studies suggest that excess iodide causes developmental arrest largely independent of changes in gene expression, and wdr-23(lf) could affect the expression of a subset of genes by a mechanism different from SKN-1 activation.

Organism:

Caenorhabditis elegans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18245

18 Samples

Download data: XLS

(Submitter supplied) Hydrogen sulfide (H2S) has dramatic physiological effects on animals that are associated with improved survival in changing conditions. C. elegans grown in H2S are long-lived and thermotolerant (1). To begin to identify mechanisms by which adaptation to H2S effects fundamental physiological functions, we have defined transcriptional changes associated with the response to H2S. Using microarray analysis we observe rapid changes in the abundance of specific mRNAs. more...

Organism:

Caenorhabditis elegans

Type:

Expression profiling by array

Platform:

GPL11171

3 Samples

Download data: PAIR