GEO DataSets

Analysis of lymphatic endothelial cells (LECs) depleted for Prox1, NR2F2, or both regulators. LECs are derived from venous endothelial cells (VECs). Prox1 and NR2F2 regulate LEC and VEC development, respectively. Results provide insight into the role of NR2F2 and Prox1 in maintaining LEC phenotypes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 protocol sets

Platform:

GPL570

Series:

GSE12846

4 Samples

Download data: CEL, CHP

(Submitter supplied) Human Notch1 intracellular domain (NICD) was overexpressed in human primary lymphatic endothelial cells (LECs) for 10 and 24 hours by adenovirus. A GFP-control adenovirus-infected cells (24hours) and uninfected cells were also analysed as controls. Total RNAs were harvested and subjected to Affymetrix U133A microarray.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

4 Samples

Download data

(Submitter supplied) Gene expression profiles of primary lymphatic endothelial cells (LECs) isolated from human foreskin were analyzed after siRNA-mediated knockdown of control (firefly luciferase), Prox1, NR2F2 or Prox1/NR2F2 for 48 hours.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3534

Platform:

GPL570

4 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array; Expression profiling by array

Platforms:

GPL570 GPL8617

47 Samples

Download data: CEL, TXT

(Submitter supplied) Kaposi sarcoma is the most common cancer in AIDS patients and is typified by red skin lesions. The disease is caused by the KSHV virus (HHV8) and is recognisable by its distinctive red skin lesions. The lesions are KSHV-infected spindle cells, most commonly the lymphatic endothelial and blood vessel endothelial cells (LEC and BEC), plus surrounding stroma. The KSHV virus expresses multiple MAF-downregulating microRNA. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data: CEL

(Submitter supplied) Kaposi sarcoma is the most common cancer in AIDS patients and is typified by red skin lesions. The disease is caused by the KSHV virus (HHV8) and is recognisable by its distinctive red skin lesions. The lesions are KSHV-infected spindle cells, most commonly the lymphatic endothelial and blood vessel endothelial cells (LEC and BEC), plus surrounding stroma. The KSHV virus expresses multiple microRNA in a single cluster. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data: CEL

(Submitter supplied) Kaposi sarcoma is the most common cancer in AIDS patients and is typified by red skin lesions. The disease is caused by the KSHV virus (HHV8) and is recognisable by its distinctive red skin lesions. The lesions are KSHV-infected spindle cells, most commonly the lymphatic endothelial and blood vessel endothelial cells (LEC and BEC), plus surrounding stroma. The effects of KSHV infection of both LEC and BEC were assayed using Affymetrix hgu133plus2 chips at 72 hours post infection.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

24 Samples

Download data: CEL

(Submitter supplied) Kaposi sarcoma is the most common cancer in AIDS patients and is typified by red skin lesions. The disease is caused by the KSHV virus (HHV8) and is recognisable by its distinctive red skin lesions. The lesions are KSHV-infected spindle cells, most commonly the lymphatic endothelial and blood vessel endothelial cells (LEC and BEC), plus surrounding stroma. Here we study the microRNA profiles of the KS lesion biopsies in AIDS patients (including both the cellular and KSHV microRNA).

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL8617

11 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Human herpesvirus 8 type M; Human herpesvirus 8 strain rKSHV.219; Homo sapiens; Human gammaherpesvirus 8

Type:

Expression profiling by genome tiling array

Platforms:

GPL16894 GPL10083

15 Samples

Download data: TXT

(Submitter supplied) Expression profiling of stably infected epithelial cells using a custom tiling microarray. iSLK was infected with rKSHV.219 and selected with puromycin. Mock infected iSLK served as control for iSLK.219. Lytic reactivation of iSLK.219 was induced with 1 ug/mL doxycycline for 48 hours.

Organism:

Human gammaherpesvirus 8; Homo sapiens; Human herpesvirus 8 type M; Human herpesvirus 8 strain rKSHV.219

Type:

Expression profiling by genome tiling array

Platform:

GPL10083

3 Samples

Download data: TXT

(Submitter supplied) Expression profiling of stably infected primary endothelial cells using a custom tiling microarray. LEC, BEC, HUVEC, and HAEC were infected with rKSHV.219 and selected with puromycin for 2 weeks. Mock infected LEC, BEC, HUVEC, and HAEC served as controls for their infected counterparts. All cells were harvested after 2 weeks of stable infection.

Organism:

Human herpesvirus 8 strain rKSHV.219; Homo sapiens; Human gammaherpesvirus 8

Type:

Expression profiling by genome tiling array

Platform:

GPL16894

8 Samples

Download data: TXT

(Submitter supplied) Expression profiling of stably infected primary endothelial cells using a custom tiling microarray. LEC and BEC were infected with rKSHV.219 and selected with puromycin for 2 weeks. Mock infected LEC and BEC served as controls for their infected counterparts. All cells were harvested after 2 weeks of stable infection.

Organism:

Human gammaherpesvirus 8; Human herpesvirus 8 strain rKSHV.219; Homo sapiens

Type:

Expression profiling by genome tiling array

Platform:

GPL16894

4 Samples

Download data: TXT

(Submitter supplied) The lymphatic vascular system maintains tissue fluid homeostasis, helps mediate afferent immune responses and promotes cancer metastasis. MicroRNAs (miRNAs) have recently emerged as key and potent regulators of the genome that control virtually all aspects of cell and organism biology. Surprisingly, the physiological importance and functional activities of miRNAs in the lymphatic vascular system have not been explored. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL2986

4 Samples

Download data: TXT

(Submitter supplied) We performed high throughput RNA-sequencing on KSHV-infected blood and lymphatic HMVECs at 48hpi as well as WTKSHV- or KSHVDvcyc-infected lymphatic HMVECS at 1wpi to identify differences in gene expression induced by KSHV in these two cell types and gene expression changes that require the KSHV latent gene vcyclin.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

12 Samples

Download data: TXT

(Submitter supplied) The identification of circulating endothelial progenitor cells has led to speculation regarding their origin as well as their contribution to neovascular development. Two distinct types of endothelium make up the blood and lymphatic vessel system. However, it has yet to be determined whether there are distinct lymphatic-specific circulating endothelial progenitor cells. We isolated circulating endothelial colony forming cells (ECFCs) from whole peripheral blood. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Danio rerio

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:

GPL20828

11 Samples

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(Submitter supplied) During development, lymphatic vasculature forms as a second and distinct network that derives from embryonic blood vessels. Transdifferentiation of venous endothelial cells into specified lymphatic endothelial cells (LECs) is the first step in this process. Transdifferentiation and specification of LEC fate requires Prox1, but how Prox1 regulates transdifferentiation and differentiation is not fully understood. more...

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20828

9 Samples

Download data

(Submitter supplied) During development, lymphatic vasculature forms as a second and distinct network that derives from embryonic blood vessels. Transdifferentiation of venous endothelial cells into specified lymphatic endothelial cells (LECs) is the first step in this process. Transdifferentiation and specification of LEC fate requires Prox1, but how Prox1 regulates transdifferentiation and differentiation is not fully understood. more...

Organism:

Danio rerio

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL20828

2 Samples

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(Submitter supplied) We made intestinal organoid cultures from Villin-CreERT2;Apcflox/flox mice. Organoids were subjected to 4-OH-Tam treatment, whereafter Apc mutant organoids were selected in R-Spondin free medium. DBZ or DMSO treatment was started on day 3. On day 8, organoids were dissociated to obtain single cells and analyzed using the Chromium Single-cell 3'RNA-sequencing system

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

2 Samples

Download data: TXT

(Submitter supplied) We isolated and selected intestinal adenoma organoids from Apcmin/+; Rosa26LSL-TdTomato; Prox1-CreERT2 mice. After the selection procedure without growth factors, we induced CreERT2 activity and the transcription of tdTomato to label Prox1+ cells by 300 nM 4-hydroxytamoxifen for 16h. tdTomato+ (Prox1+) and tdTomato- cells (enriched for Prox1- cells) were FACS sorted and total RNA was isolated.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6887

6 Samples

Download data: TXT