GEO DataSets

(Submitter supplied) The life-threatening pathogen Leptospira interrogans navigates a dual existence: surviving in environmental reservoirs and infecting mammalian hosts. Leptospira biofilm formation is thought to be an important survival strategy in environmental contexts and may also contribute to the persistence of leptospirosis in maintenance hosts. Examining the correlation between biofilm formation and the virulence of pathogenic strains might improve our comprehension of the epidemiology of leptospirosis. more...

Organism:

Leptospira interrogans serovar Manilae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL34667

8 Samples

Download data: TXT

(Submitter supplied) Polyamines, crucial molecules involved in cell proliferation and growth, play a pivotal role in cancer development and progression. Within the tumor microenvironment, macrophages, key components of the immune system, exhibit a complex relationship with polyamines. Evidence suggests that polyamines can modulate macrophage polarization, influencing their functional phenotypes. Here, we detected the gene DNA methylation changes in spermine-stimulated human macrophages isolated from PBMCs and TAMs.

Organism:

Campylobacter jejuni; Francisella tularensis subsp. novicida; Yersinia pestis; Staphylococcus aureus; Mycobacterium avium subsp. paratuberculosis; Cowpox virus; Escherichia coli O157:H7; Francisella tularensis subsp. mediasiatica; Paslahepevirus balayani; Yersinia enterocolitica; Toxoplasma gondii; Salmonella enterica subsp. enterica serovar Typhimurium; Mammarenavirus choriomeningitidis; Orthohantavirus puumalaense; Yersinia pseudotuberculosis; Rickettsia prowazekii; Bartonella quintana; Mycobacterium avium; Homo sapiens; Streptobacillus moniliformis; Bartonella henselae; Francisella tularensis subsp. tularensis; Francisella tularensis subsp. holarctica; Leptospira interrogans; Rickettsia typhi; Mycobacterium tuberculosis variant bovis; Mycobacterium tuberculosis; Mycobacterium tuberculosis variant microti; Mycobacterium canetti; Orthohantavirus seoulense

Type:

Methylation profiling by array

Platform:

GPL21445

4 Samples

Download data: IDAT, TXT

(Submitter supplied) To better understand the mechanisms underlying the motility phenotype observed in the flhG mutant, we used RNA-Seq to compare the transcriptomes of wild-type L. biflexa with that of the flhG and hfq-like mutants under ideal culture conditions at mid-log phase.

Organism:

Leptospira biflexa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29973

8 Samples

Download data: TXT

(Submitter supplied) Pathogenic Leptospira spp. are the causative agents of the zoonotic disease leptospirosis. During infection, Leptospira are confronted with deadly reactive oxygen species (ROS). Withstanding ROS produced by the host innate immunity is an important strategy evolved by pathogenic Leptospira for persisting in and colonizing hosts. The peroxide stress regulator, PerRA, represses genes involved in ROS defenses in L. more...

Organism:

Leptospira interrogans serovar Manilae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21582

12 Samples

Download data: TXT

(Submitter supplied) Leptospirosis is a global zoonotic, neglected tropical disease. Interestingly, a high level of species specificity (both bacteria and host) plays a major role in the severity of disease presentation which can vary from asymptomatic to multi-organ failure. Pathogenic Leptospira colonize the kidneys of infected individuals and are shed in urine into the environment where they can survive until they are contracted by another host. more...

Organism:

Leptospira borgpetersenii serovar Hardjo

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29825

16 Samples

Download data: TXT

(Submitter supplied) Transcriptome analysis of a mutant in an orphan methyltranferase revealed global transcriptional regulation of 100 differentially expressed genes.

Organism:

Leptospira interrogans serovar Manilae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21582

9 Samples

Download data: FA, GFF, XLS

(Submitter supplied) Pathogenic Leptospira spp. are the causative agents of the zoonotic disease leptospirosis. During infection, Leptospira are confronted with deadly reactive oxygen species (ROS). Withstanding ROS produced by the host innate immunity is an important strategy evolved by pathogenic Leptospira for persisting in and colonizing hosts. The peroxide stress regulator, PerR, represses genes involved in ROS defenses in L. more...

Organism:

Leptospira interrogans serovar Manilae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21582

24 Samples

Download data: TXT

(Submitter supplied) Transmission of leptospirosis requires that the spirochete pathogen adapts rapidly to the mammalian host milieu during infection and the external environment upon shedding from the renal tubules of animal reservoirs. The pathogenic Leptospira genomes encode a notably large number (≥76) of putative two-component system (TCS) proteins, which presumably play a key role in switching the ecological niches. more...

Organism:

Leptospira interrogans serovar Manilae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21582

6 Samples

Download data: TXT

(Submitter supplied) Leptospira are emerging zoonotic pathogens transmitted from animals to humans typically through contaminated environmental sources of water and soil. Transcriptional regulation of pathogenic Leptospira spp. underlying the adaptive response to different hosts and environmental conditions remains elusive. In this study, we provide the first global Transcriptional Start Site (TSS) map of a Leptospira species. more...

Organism:

Leptospira interrogans serovar Manilae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21582

8 Samples

Download data: WIG

(Submitter supplied) Leptospirosis is zoonotic disease of global importance, with over a million cases andnearly 60,000 deaths annually. Symptomatic disease presentation ranges from a mildfebrile disease with non-specific symptoms to severe forms, characterized by multi-organ failure, lung hemorrhage, and death. Factors governing severe outcomes remainunclear, but the host immune response likely plays an important role. more...

Organism:

Leptospira interrogans serovar Copenhageni; Homo sapiens

Type:

Protein profiling by protein array

Platform:

GPL22411

389 Samples

Download data: TXT

(Submitter supplied) A microarray was developed to screen rodent samples for pathogens of zoonotic importance In the work described here, a homologue to Yersinia pestis was found in rodent samples after screening with the microarray

Organism:

Yersinia enterocolitica; Rickettsia prowazekii; Rickettsia typhi; Mycobacterium tuberculosis variant bovis; Toxoplasma gondii; Apodemus sylvaticus; Salmonella enterica subsp. enterica serovar Typhimurium; Leptospira interrogans; Francisella tularensis subsp. novicida; Yersinia pseudotuberculosis; Mycobacterium avium subsp. paratuberculosis; Mycobacterium tuberculosis; Mus musculus; Streptobacillus moniliformis; Bartonella henselae; Francisella tularensis subsp. tularensis; Paslahepevirus balayani; Campylobacter jejuni; Yersinia pestis; Mycobacterium tuberculosis variant microti; Rattus rattus; Mycobacterium canetti; Francisella tularensis subsp. mediasiatica; Mammarenavirus choriomeningitidis; Orthohantavirus puumalaense; Orthohantavirus seoulense; Bartonella quintana; Staphylococcus aureus; Mycobacterium avium; Rattus norvegicus; Cowpox virus; Escherichia coli O157:H7; Francisella tularensis subsp. holarctica

Type:

Genome variation profiling by array

Platform:

GPL21445

65 Samples

Download data: TXT

(Submitter supplied) The pathogen L. interrogans serovar Copenhageni can be shed in the urine of carrier mammals, and infect humans after weeks on water or mud. This wide range of possible niches implies the bacterium can be exposed to several different genotoxic stresses during its lifecycle. We assessed the impact of DNA damage in this leptospire, integrating high throughput data from ChIP-seq and DNA microarrays to determine the role of the SOS regulator LexA1. more...

Organism:

Leptospira interrogans serovar Copenhageni

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL20985

4 Samples

Download data: TXT

(Submitter supplied) The pathogen L. interrogans serovar Copenhageni can be shed in the urine of carrier mammals, and infect humans after weeks on water or mud. This wide range of possible niches implies the bacterium can be exposed to several different genotoxic stresses during its lifecycle. We assessed the impact of DNA damage in this leptospire, integrating high throughput data from ChIP-seq and DNA microarrays to determine the role of the SOS regulator LexA1. more...

Organism:

Leptospira interrogans serovar Copenhageni; Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130

Type:

Expression profiling by array

Platform:

GPL20984

8 Samples

Download data: TXT

(Submitter supplied) Leptospirosis, an emerging zoonotic disease with worldwide distribution, is caused by spirochetes belonging to the genus Leptospira. More than 500,000 cases of severe leptospirosis are reported annually, with .10% of these being fatal. Leptospires can survive for weeks in suitably moist conditions before encountering a new host. Reservoir hosts, typically rodents, exhibit little to no signs of disease but shed large numbers of organisms in their urine. more...

Organism:

Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18135

6 Samples

Download data: GR

(Submitter supplied) Leptospirosis is the most widespread zoonotic disease in the world. The lack of an adequate laboratory test is a major barrier for the diagnosis, especially during the early stages of illness, when antibiotic therapy is most effective. Therefore, there is a critical need for an efficient diagnostic test for this life threatening disease. In order to identify new targets that could be used as diagnostic makers for leptopirosis, we constructed a protein microarray chip comprising 61% of Leptospira interrogans proteome and investigated the IgG response against leptospiral antigens from 274 individuals, including 80 acute-, 80 convalescent-phase patients and 114 healthy control subjects from regions with endemic, high endemic and no endemic transmission of leptospirosis. more...

Organism:

Homo sapiens; Leptospira interrogans serovar Copenhageni

Type:

Protein profiling by protein array

Platform:

GPL16351

274 Samples

Download data: TXT

(Submitter supplied) L. interrogans, a causative agent of leptospirosis, can survive in the environment for lengthy periods of time in between infection of mammalian hosts. In order to identify genes involved in survival in the early spirochetemic phase of infection, we performed a transcriptional analysis of L. interrogans serovar Copenhageni upon exposure to serum in comparison with EMJH medium.

Organism:

Leptospira interrogans

Type:

Expression profiling by array

Platform:

GPL9140

3 Samples

Download data: TXT

(Submitter supplied) Leptospirosis is a globally significant zoonotic disease caused by Leptospira spp. Iron is essential for growth of most bacterial species. Since availability of iron is low in the host, pathogens have evolved complex iron acquisition mechanisms to survive and establish infection. Virulence genes in some bacteria have been shown to be iron-regulated. In many bacteria, expression of iron-uptake and storage proteins is regulated by Fur. more...

Organism:

Leptospira interrogans

Type:

Expression profiling by array

Platform:

GPL10072

6 Samples

Download data: TXT

(Submitter supplied) DNA microarray analysis was used to compare the differential gene-expression profiles between Leptospira interrogans serovar Lai type strain 56601 and its corresponding attenuated strain IPAV. A 22 kb genomic island covering a cluster of 34 genes (LA0186 to LA0219) was actively expressed in both strains but concomitantly up-expressed in strain 56601 in contrast to that of IPAV. RT-PCR assays proved that the gene cluster comprised five transcripts. more...

Organism:

Leptospira interrogans serovar Lai; Leptospira interrogans serovar Lai str. 56601; Leptospira interrogans

Type:

Expression profiling by array

Platform:

GPL6571

3 Samples

Download data: TXT