GEO DataSets

(Submitter supplied) We have developed the vaccine candidate BCGΔBCG1419c, by deletion of BCG1419c in BCG Pasteur ATCC 35734, which improved control of tuberculosis (TB) in preclinical models. Here, we compared the transcriptomes of BCG and BCGΔBCG1419c during planktonic growth.

Organism:

Mycobacterium tuberculosis variant bovis BCG

Type:

Expression profiling by high throughput sequencing

Platform:

GPL34031

6 Samples

Download data: TXT

(Submitter supplied) We analyzed the genes expressed, or the transcriptome, of bacilli Mycobacterium bovis BCG Pasteur (wtBCG) and a mutant strain obtained by transposition of the gene BCG_ BCG_2177c (mtBCG), cultured in the presence of a lipid mixture (fatty acids/cholesterol) as main carbon source. Using RNAseq we identified a group of genes that provides novel insight regarding the metabolic pathways and transcriptional regulation, and pathogenic features that were influenced by BCG_2177c gene, a TetR-like repressor, during the lipid metabolism in slow-growing mycobacteria that could be extrapolated to other members of the MTBC.

Organism:

Mycobacterium tuberculosis variant bovis BCG

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29423

8 Samples

Download data: TXT

(Submitter supplied) This study aimed to construct a rapid screening system to identify novel anti-TB agents from a library of small-molecule compounds.

Organism:

Mycobacterium tuberculosis variant bovis BCG

Type:

Expression profiling by high throughput sequencing

Platform:

GPL31041

17 Samples

Download data: TXT

(Submitter supplied) Intracellular pathogens requires efficient mechanism adapting the environment inside of host cells. Here we show that the transcriptomes of the cellular mycobacterium tuberculosis and of those released from the infected macrophage cells contain a large fraction of human transcripts by RNA polymerase II. Uptake of host pre-mRNAs is coincident with the co-localization of M. tuberculosis with nuclear membrane. more...

Organism:

Homo sapiens; Mycobacterium tuberculosis M1458; Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Type:

Expression profiling by high throughput sequencing

4 related Platforms

22 Samples

Download data: XLSX

(Submitter supplied) Objective of the study is to find out how c-di-GMP regulate bacteria under hypoxic conditions and new c-di-GMP receptors.

Organism:

Mycobacterium tuberculosis variant bovis BCG

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29423

6 Samples

Download data: XLSX

(Submitter supplied) The goal of this study aims to quantitatively compare the gene expression difference in wild-type or cmtR-deleted strains of Mycobacterium bovis BCG with and without excess zinc treatment at the transcriptome level and find the underlying mechanism how could excess zinc inhibit mycobacterial growth and its relation with transcription factor CmtR.

Organism:

Mycobacterium tuberculosis variant bovis BCG

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30387

12 Samples

Download data: TXT

(Submitter supplied) M. bovis BCG bacilli were subjected to silencing of the darG antitoxin gene by CRISPRI, leading to unregulated activity of the toxin DarT. This results in ADP-ribosylation of specific motifs in genomic DNA, and induction of the DNA damage response. The goal of this study is to compare gene expression of M. bovis BCG in untreated, DarG-depleted, and mitomycin treated samples.

Organism:

Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30131

9 Samples

Download data: TXT

(Submitter supplied) By RNA-seq analysis, we monitored the early steps of biofilm production in BCG, to distinguish intercellular aggregation from attachment to a surface, and found a number of genes being differentially expressed at these stages.

Organism:

Mycobacterium tuberculosis variant bovis BCG

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28494

6 Samples

Download data: TXT

(Submitter supplied) Comparison of transcriptional differences (RNA-seq) between BCG delta1419c and wild-type BCG that might explain the pathological differences in vaccination studies.

Organism:

Mycobacterium tuberculosis variant bovis BCG

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24951

8 Samples

Download data: TXT

(Submitter supplied) We identified the genome-wide binding sites of CRP/FNR family transcription factors CRP and Cmr in M. bovis BCG using ChIP-seq, and cross-referenced these binding sites to orthologous regions in M. tuberculosis. Selected binding sites for each transcription factor were further characterized for their importance to gene regulation.

Organism:

Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL24180

10 Samples

Download data: TXT

(Submitter supplied) Mycobacterial pathogens adapt to environmental stresses such as nutrient deprivation by entering a non-replicative antibiotic-tolerant state of persistence. Using a biochemically-validated data-driven approach, we identified an adaptive metabolic network underlying the mycobacterial response to starvation in M. tuberculosis, M. bovis BCG and M. smegmatis. All three species show a strong Mg+2-dependence for surviving complete nutrient deprivation, accompanied by a broad phenotypic antibiotic resistance. more...

Organism:

Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19889

15 Samples

Download data: TXT

(Submitter supplied) To further determine the gene expression profile of InbR, encoded by Rv0275c in Mycobacterium tuberculosis, whole genome microarray analysises were applied to inbR-overexpressing strain and wild type Mycobacterium bovis BCG. Comparison of the gene expression pattern of logarithmic growth phase wild-type M. bovis BCG and that of the inbR-overexpression strain revealed a total of 142 genes that were significantly up-regulated (2-fold to 187-fold, p < 0.05) and 200 that were down-regulated (2-fold to 113-fold, p < 0.05). more...

Organism:

Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2; Mycobacterium tuberculosis variant bovis BCG

Type:

Expression profiling by array

Platform:

GPL20248

8 Samples

Download data: TXT

(Submitter supplied) Some intracellular bacteria are known to cause long-term infections for periods of time that last decades without compromising the viability of the host. Although of critical importance, the changes that intracellular bacteria suffer during this long process of residence in a host cell environment remain obscure. Here, we report an experimental approach to study the adaptations of intracellular mycobacteria forced by a long-term intracellular lifestyle. more...

Organism:

Mycobacterium tuberculosis variant bovis BCG

Type:

Expression profiling by array

Platform:

GPL17588

14 Samples

Download data: TXT

(Submitter supplied) Global gene expression analysis of Mycobacterium bovis BCG following Triclosan treatment using Affymetrix GeneChip arrays. Results from this study provide insight into the molecular mechanisms underlying the cellular response of Mycobacterium bovis BCG to Triclosan

Organism:

Mycobacterium tuberculosis variant bovis BCG

Type:

Expression profiling by array

Platform:

GPL7549

9 Samples

Download data: CEL

(Submitter supplied) We used DNA microarrays to define the physiological roles of the Tap efflux pump in M. bovis BCG during the exponential and the stationary phase of in vitro growth. For this purpose we constructed a M. bovis BCG strain in which the tap gene was inactivated by the insertion of a hygromycin resistance cassette (Ω-hyg). When the gene expression patterns of the tap mutant were compared to the wild-type strain, almost no differences were observed during exponential growth; only seven genes slightly increased their expression. more...

Organism:

Mycobacterium tuberculosis variant bovis BCG; Mycobacterium tuberculosis

Type:

Expression profiling by array

Platform:

GPL4057

8 Samples

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(Submitter supplied) Objective of the study is to find out the differentially regulated genes in Mycobacterium bovis BCG subjected to hypoxic condition. Gene expression profiling was carried out using Agilent microarray platform. Keywords: Hypoxia response

Organism:

Mycobacterium tuberculosis H37Rv; Mycobacterium tuberculosis variant bovis BCG

Type:

Expression profiling by array

Platform:

GPL8431

2 Samples

Download data: TXT

(Submitter supplied) Objective of the study is to find out the differentially regulated genes of Mycobacterium BCG strain in extended stationary phase comparison to log phase growth and resuscitation phase. Mycobacterium BCG culture was grown in Sauton medium at 37o C without shaking. Cells at A600 0.6-0.8 were harvested as log phase culture. The cells harvested after 5 months incubation at 37oC without shaking became non culturable and were harvested as extended stationary phase cells. more...

Organism:

Mycobacterium tuberculosis variant bovis BCG; Mycobacterium tuberculosis H37Rv

Type:

Expression profiling by array

Platform:

GPL8431

4 Samples

Download data: TXT

(Submitter supplied) In the present study, we employed Affymetrix Mycobacterium bovis BCG GeneChip arrays to investigate the dynamics of global gene expression profiles during the cellular response of Mycobacterium bovis BCG to Peracetic acid, which involved initial growth inhibition and metabolism. Keywords: Transcriptome study

Organism:

Mycobacterium tuberculosis variant bovis BCG

Type:

Expression profiling by array

Platform:

GPL7549

9 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mycobacterium tuberculosis variant bovis BCG; Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2

Type:

Expression profiling by array

Platform:

GPL7549

15 Samples

Download data: CEL

(Submitter supplied) In the present study, we employed Affymetrix Mycobacterium bovis BCG GeneChip arrays to investigate the dynamics of global gene expression profiles during the cellular response of Mycobacterium bovis BCG to hydrogen peroxide, which involved initial growth inhibition and metabolism. Keywords: Transcriptome study

Organism:

Mycobacterium tuberculosis variant bovis BCG; Mycobacterium tuberculosis variant bovis BCG str. Pasteur 1173P2

Type:

Expression profiling by array

Platform:

GPL7549

9 Samples

Download data: CEL