brp bruchpilot [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: brpprovided by FlyBase
Official Full Name: bruchpilotprovided by FlyBase
Primary source: FLYBASE:FBgn0259246
Locus tag: Dmel_CG42344
See related: AllianceGenome:FB:FBgn0259246
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: Brp; BRP; Bruchpilot; Cast; CG12932; CG12933; CG1931; CG30336; CG30337; CG34146; CG42344; Dmel\CG42344; mAb nc82; mAbnc82; Mabnc82; MAbnc82; nc-82; nc82; Nc82; NC82
Old locus tag: Dmel_CG12932; Dmel_CG34146
Summary: Predicted to be a structural constituent of presynaptic active zone. Involved in several processes, including chemical synaptic transmission; cytoskeletal matrix organization at active zone; and memory. Located in several cellular components, including cytoskeleton of presynaptic active zone; neuromuscular junction; and presynaptic membrane. Is expressed in several structures, including Kenyon cell; adult brain; adult head; outer photoreceptor cell; and synaptic neuropil domain. Orthologous to human ERC1 (ELKS/RAB6-interacting/CAST family member 1) and ERC2 (ELKS/RAB6-interacting/CAST family member 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 45E1-45F1; 2-60 cM

Exon count:: 26

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (9503765..9541565)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (5391270..5429070)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Complexin cooperates with Bruchpilot to tether synaptic vesicles to the active zone cytomatrix.
Title: Complexin cooperates with Bruchpilot to tether synaptic vesicles to the active zone cytomatrix.
localization of BRP-an essential active zone scaffolding protein in Drosophila, depends on the precise balance of neuronal Par-1 kinase.
Title: Levels of Par-1 kinase determine the localization of Bruchpilot at the Drosophila neuromuscular junction synapses.
Consistent with a block in axonal transport, we find a decrease in number of active zones and reduced neurotransmission in flies overexpressing Par-1 kinase. Interestingly, we find that Par-1 acts independently of Tau-one of the most well studied substrates of Par-1, revealing a presynaptic function for Par-1 that is independent of Tau
Title: Active zone proteins are transported via distinct mechanisms regulated by Par-1 kinase.
The presynaptic protein Brp promotes evoked transmission and suppresses spontaneous release.
Title: Evoked and spontaneous transmission favored by distinct sets of synapses.
The precise nanoscopic organization of Brp distinguishes different physiological active zones (AZs) and links functional diversification to a heretofore unrecognized neuronal gradient of the AZ cytomatrix ultrastructure.
Title: Quantitative super-resolution imaging of Bruchpilot distinguishes active zone states.
CK2alpha is a novel regulator of the active zone protein, BRP, in Drosophila.
Title: CK2α regulates the transcription of BRP in Drosophila.
The macromolecular architecture created by the alternating pattern of the Bruchpilot isoforms determines the number of Ca(2+) channel-coupled SV release slots available per AZ and thereby sets the size of the RRP.
Title: The Bruchpilot cytomatrix determines the size of the readily releasable pool of synaptic vesicles.
This study found a circadian rhythm of BRP expression in the first optic neuropil (lamina) of the optic lobe and in the brain of D. melanogaster. In LD 12:12 the rhythm of BRP abundance is bimodal, displaying morning (ZT1) and evening (ZT13) peaks
Title: Circadian expression of the presynaptic active zone protein Bruchpilot in the lamina of Drosophila melanogaster.
Review describes how genetic elimination of Bruchpilot revealed that the protein is essential for T-bar formation, calcium channel clustering, and hence proper vesicle fusion and patterned synaptic plasticity.
Title: The active zone T-bar--a plasticity module?
Bruchpilot, a ubiquitous presynaptic active zone protein, is required for anesthesia-resistant olfactory memory.
Title: Bruchpilot, a synaptic active zone protein for anesthesia-resistant memory.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables structural constituent of presynaptic active zone	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in adult locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in anesthesia-resistant memory	IDA Inferred from Direct Assay more info	PubMed
involved_in chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cytoskeletal matrix organization at active zone	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maintenance of presynaptic active zone structure	IBA Inferred from Biological aspect of Ancestor more info
involved_in maintenance of presynaptic active zone structure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuromuscular synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neurotransmitter secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in presynaptic active zone organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of synaptic plasticity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in short-term memory	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of synapse maturation	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in axon terminus	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton of presynaptic active zone	IDA Inferred from Direct Assay more info	PubMed
located_in neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed
located_in presynaptic active zone	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynaptic active zone cytoplasmic component	IBA Inferred from Biological aspect of Ancestor more info
located_in presynaptic membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: bruchpilot

Names: CG42344-PD; CG42344-PE; CG42344-PG; CG42344-PH; CG42344-PI; CG42344-PJ; CG42344-PK; CG42344-PL; CG42344-PM; CG42344-PN; CG42344-PO; CG42344-PP; CG42344-PQ; CG42344-PR; brp-PD; brp-PE; brp-PG; brp-PH; brp-PI; brp-PJ; brp-PK; brp-PL; brp-PM; brp-PN; brp-PO; brp-PP; brp-PQ; brp-PR; brunchpilot; burchpilot; monoclonal antibody nc82; nc82

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

9503765..9541565

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001414094.1 → NP_001400998.1 bruchpilot, isoform N [Drosophila melanogaster]

Status: REVIEWED
NM_001259299.3 → NP_001246228.1 bruchpilot, isoform J [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246228.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K7K9

Conserved Domains (4) summary

pfam11759
Location:332 → 401

KRTAP; Keratin-associated matrix

TIGR02168
Location:1509 → 2215

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam01576
Location:789 → 1824

Myosin_tail_1; Myosin tail

pfam09736
Location:2091 → 2157

Bud13; Pre-mRNA-splicing factor of RES complex
NM_001414096.1 → NP_001401000.1 bruchpilot, isoform P [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

Q25B55
NM_001414097.1 → NP_001401001.1 bruchpilot, isoform Q [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

Q25B55
NM_001414098.1 → NP_001401002.1 bruchpilot, isoform R [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

Q25B55
NM_001414095.1 → NP_001400999.1 bruchpilot, isoform O [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

Q25B55
NM_001273897.2 → NP_001260826.1 bruchpilot, isoform M [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260826.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KF38, Q25B55

Conserved Domains (3) summary

COG1196
Location:813 → 1469

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:427 → 1133

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam10174
Location:115 → 663

Cast; RIM-binding protein of the cytomatrix active zone
NM_165677.4 → NP_724796.3 bruchpilot, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_724796.3

Status: REVIEWED

UniProtKB/TrEMBL

A1Z7V1, Q25B55

Conserved Domains (3) summary

COG1196
Location:773 → 1616

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:372 → 1161

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam10174
Location:115 → 695

Cast; RIM-binding protein of the cytomatrix active zone
NM_001103771.4 → NP_001097241.2 bruchpilot, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001097241.2

Status: REVIEWED

UniProtKB/TrEMBL

A8DY79, Q25B55

Conserved Domains (3) summary

COG1196
Location:819 → 1662

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:418 → 1207

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam10174
Location:161 → 741

Cast; RIM-binding protein of the cytomatrix active zone
NM_001259301.2 → NP_001246230.1 bruchpilot, isoform L [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246230.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K6V0, Q25B55

Conserved Domains (3) summary

COG1196
Location:814 → 1657

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:415 → 1202

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam10174
Location:161 → 736

Cast; RIM-binding protein of the cytomatrix active zone
NM_001259300.2 → NP_001246229.1 bruchpilot, isoform K [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246229.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K7V8, Q25B55

Conserved Domains (3) summary

TIGR02168
Location:978 → 1684

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

TIGR02169
Location:554 → 1296

SMC_prok_A; chromosome segregation protein SMC, primarily archaeal type

pfam00261
Location:487 → 721

Tropomyosin; Tropomyosin
NM_206063.3 → NP_995785.2 bruchpilot, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_995785.2

Status: REVIEWED

UniProtKB/TrEMBL

A1Z7V1, Q25B55

Conserved Domains (3) summary

COG1196
Location:773 → 1616

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:372 → 1161

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam10174
Location:115 → 695

Cast; RIM-binding protein of the cytomatrix active zone
NM_001043070.1 → NP_001036535.1 bruchpilot, isoform H [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001036535.1

Status: REVIEWED

UniProtKB/TrEMBL

A1Z7V1, Q25B55

Conserved Domains (3) summary

COG1196
Location:773 → 1616

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

TIGR02168
Location:372 → 1161

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam10174
Location:115 → 695

Cast; RIM-binding protein of the cytomatrix active zone
NM_001259298.2 → NP_001246227.1 bruchpilot, isoform I [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246227.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K843, Q25B55

Conserved Domains (2) summary

TIGR02168
Location:149 → 926

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam10174
Location:9 → 415

Cast; RIM-binding protein of the cytomatrix active zone

Nucleotide		Protein
Heading	Accession and Version	Protein

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
A0A0B4K6V0		A0A0B4K6V0
A0A0B4K7K9		A0A0B4K7K9
A0A0B4K7V8		A0A0B4K7V8
A0A0B4K843		A0A0B4K843
A0A0B4KF38		A0A0B4KF38
A1Z7V1		A1Z7V1
A8DY79		A8DY79
Q25B55		Q25B55
Q8MRS3		Q8MRS3