Pfk Phosphofructokinase [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Pfkprovided by FlyBase
Official Full Name: Phosphofructokinaseprovided by FlyBase
Primary source: FLYBASE:FBgn0003071
Locus tag: Dmel_CG4001
See related: AllianceGenome:FB:FBgn0003071
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: BcDNA.GH12192; BcDNA:GH12192; CG4001; Dmel\CG4001; l(2)06339; pfk; PFK; PFK1
Summary: Enables 6-phosphofructokinase activity. Involved in several processes, including fructose 1,6-bisphosphate metabolic process; fructose 6-phosphate metabolic process; and glycolytic process. Predicted to be located in cytosol. Predicted to be part of 6-phosphofructokinase complex. Is expressed in several structures, including adult head; adult heart; larval muscle system; muscle cell; and spermatozoon. Used to study disease of metabolism. Human ortholog(s) of this gene implicated in glycogen storage disease VII. Orthologous to several human genes including PFKL (phosphofructokinase, liver type) and PFKM (phosphofructokinase, muscle). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 46E8-46F1; 2-62 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (10109740..10117457)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (5997245..6004962)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables 6-phosphofructokinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 6-phosphofructokinase activity	IDA Inferred from Direct Assay more info	PubMed
enables 6-phosphofructokinase activity	IEA Inferred from Electronic Annotation more info
enables 6-phosphofructokinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables fructose-6-phosphate binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in canonical glycolysis	IBA Inferred from Biological aspect of Ancestor more info
involved_in canonical glycolysis	ISS Inferred from Sequence or Structural Similarity more info
involved_in fructose 1,6-bisphosphate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in fructose 1,6-bisphosphate metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in fructose 6-phosphate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in fructose 6-phosphate metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in fructose 6-phosphate metabolic process	IEA Inferred from Electronic Annotation more info
involved_in glucose homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glycolytic process	IEA Inferred from Electronic Annotation more info
involved_in glycolytic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to sucrose	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of 6-phosphofructokinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of 6-phosphofructokinase complex	IC Inferred by Curator more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
located_in mitochondrion	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: phosphofructokinase

Names: 6-phosphofructokinase; CG4001-PA; CG4001-PB; CG4001-PC; CG4001-PD; CG4001-PE; Pfk-PA; Pfk-PB; Pfk-PC; Pfk-PD; Pfk-PE; phosphofructokinase 1

NP_001246234.1

EC 2.7.1.11

NP_001260847.1

EC 2.7.1.11

NP_523676.1

EC 2.7.1.11

NP_724890.1

EC 2.7.1.11

NP_724891.2

EC 2.7.1.11

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

10109740..10117457

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001273918.1 → NP_001260847.1 phosphofructokinase, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260847.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KFI7, C8VV04

Conserved Domains (2) summary

TIGR02478
Location:184 → 901

6PF1K_euk; 6-phosphofructokinase, eukaryotic type

cl00204
Location:184 → 886

PFK; Phosphofructokinase, a key regulatory enzyme in glycolysis, catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-biphosphate. The members belong to PFK family that includes ATP- and pyrophosphate (PPi)- dependent phosphofructokinases. ...
NM_001259305.1 → NP_001246234.1 phosphofructokinase, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246234.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K7L1, C8VV04

Conserved Domains (2) summary

TIGR02478
Location:180 → 929

6PF1K_euk; 6-phosphofructokinase, eukaryotic type

cl00204
Location:178 → 914

PFK; Phosphofructokinase, a key regulatory enzyme in glycolysis, catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-biphosphate. The members belong to PFK family that includes ATP- and pyrophosphate (PPi)- dependent phosphofructokinases. ...
NM_078952.3 → NP_523676.1 phosphofructokinase, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523676.1

Status: REVIEWED

UniProtKB/TrEMBL

C8VV04

Related

FBpp0087506

Conserved Domains (2) summary

TIGR02478
Location:180 → 929

6PF1K_euk; 6-phosphofructokinase, eukaryotic type

cl00204
Location:178 → 914

PFK; Phosphofructokinase, a key regulatory enzyme in glycolysis, catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-biphosphate. The members belong to PFK family that includes ATP- and pyrophosphate (PPi)- dependent phosphofructokinases. ...
NM_165746.3 → NP_724890.1 phosphofructokinase, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_724890.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P52034, Q8IH94, Q8MKV4, Q9V5G7, Q9Y100

UniProtKB/TrEMBL

C8VV04, H5V888

Related

FBpp0087508

Conserved Domains (2) summary

TIGR02478
Location:18 → 767

6PF1K_euk; 6-phosphofructokinase, eukaryotic type

cl00204
Location:16 → 752

PFK; Phosphofructokinase, a key regulatory enzyme in glycolysis, catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-biphosphate. The members belong to PFK family that includes ATP- and pyrophosphate (PPi)- dependent phosphofructokinases. ...
NM_165747.4 → NP_724891.2 phosphofructokinase, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_724891.2

Status: REVIEWED

UniProtKB/TrEMBL

C7LA80, C8VV04

Related

FBpp0087507

Conserved Domains (2) summary

TIGR02478
Location:18 → 767

6PF1K_euk; 6-phosphofructokinase, eukaryotic type

cl00204
Location:16 → 752

PFK; Phosphofructokinase, a key regulatory enzyme in glycolysis, catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-biphosphate. The members belong to PFK family that includes ATP- and pyrophosphate (PPi)- dependent phosphofructokinases. ...