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    dnaX DNA polymerase III subunit gamma;DNA polymerase III subunit tau [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945105, updated on 3-Dec-2024

    Summary

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    Official Symbol
    dnaX
    Official Full Name
    DNA polymerase III subunit gamma;DNA polymerase III subunit tau
    Primary source
    ECOCYC:EG10245
    Locus tag
    b0470
    See related
    ASAP:ABE-0001633
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    dnaZ; ECK0464
    Summary
    The tau chain is the full length protein. [More information is available at EcoGene: EG10245]. The gamma subunit of polymerase III is generated by a programmed frameshift that occurs about half the time and leads to early termination of translation and a protein that lacks the final two domains found in tau. [More information is available at EcoCyc: EG10245].
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    Genomic context

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    Sequence:
    NC_000913.3 (492092..494023)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF454 domain-containing inner membrane protein YbaN Neighboring gene adenine phosphoribosyltransferase Neighboring gene putative nucleoid-associated protein YbaB Neighboring gene recombination mediator protein RecR Neighboring gene chaperone protein HtpG

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The dnaZ protein, the gamma subunit of DNA polymerase III holoenzyme of Escherichia coli. Hübscher U, et al. J Biol Chem, 1980 Dec 25. PMID 6254978
    2. Mutator mutants of Escherichia coli carrying a defect in the DNA polymerase III tau subunit. Pham PT, et al. Mol Microbiol, 2006 Feb. PMID 16430690
    3. The opened processivity clamp slides into view. Jeruzalmi D. Proc Natl Acad Sci U S A, 2005 Oct 18. PMID 16217036, Free PMC Article
    4. Chromosomal replicases as asymmetric dimers: studies of subunit arrangement and functional consequences. McHenry CS. Mol Microbiol, 2003 Sep. PMID 12940977
    5. Nucleotide-induced conformational changes in an isolated Escherichia coli DNA polymerase III clamp loader subunit. Podobnik M, et al. Structure, 2003 Mar. PMID 12623013

    See all (158) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. study characterized the dnaXE145A clamp loader mutant; found that the naturally occurring obstacles encountered by a replication fork are not tackled in a proper way by the mutant clamp loader and suggest a role for the clamp loader in the restart of stalled replication forks
      Title: Phenotypes of dnaX(E145A) Mutant Cells Indicate that the Escherichia coli Clamp Loader Has a Role in the Restart of Stalled Replication Forks.
    2. Ribosome frameshifting during translation of bacterial dnaX can proceed via different routes, generating a variety of distinct polypeptides.
      Title: Conditional Switch between Frameshifting Regimes upon Translation of dnaX mRNA.
    3. Data show that expression from DNA polymerase III dnaX to produce gamma subunit is essential for normal cellular function.
      Title: The DNA polymerase III holoenzyme contains γ and is not a trimeric polymerase.
    4. Data show that the tau (tau), alpha (alpha), epsilon (epsilon), and theta (theta;) subunits of DNA Polymerase III can be assembled in vivo, yielding the trimeric tau3alpha3epsilon3theta;3 complex.
      Title: Simultaneous ternary extension of DNA catalyzed by a trimeric replicase assembled in vivo.
    5. Destabilization of the single-stranded DNA-chi subunit of DNA polymerase III complex in vivo produces cells with temperature- dependent cell cycle defects that appear to arise from replisome instability.
      Title: Structure of the SSB-DNA polymerase III interface and its role in DNA replication.
    6. Analyzing DnaX complexes containing one or more K51E variant DnaX subunits demonstrated that only one active ATP binding site is required to form initiation complexes, but the two additional sites increase the rate by ca 1000-fold.
      Title: Polymerase chaperoning and multiple ATPase sites enable the E. coli DNA polymerase III holoenzyme to rapidly form initiation complexes.
    7. Only one ATP-binding DnaX subunit is required for initiation complex formation by the Escherichia coli DNA polymerase III holoenzyme.
      Title: Only one ATP-binding DnaX subunit is required for initiation complex formation by the Escherichia coli DNA polymerase III holoenzyme.
    8. The DNA polymerase III clamp loading reaction can be divided into two stages: formation of a ternary clamp loader.clamp.DNA complex, and decay of the ternary complex to produce a clamp.DNA complex. Clamp release is likely to be the rate-limiting step.
      Title: Temporal correlation of DNA binding, ATP hydrolysis, and clamp release in the clamp loading reaction catalyzed by the Escherichia coli gamma complex.
    9. Data support the assumption that dnaX36-mediated mutations originate as errors of replication rather than DNA damage-related events.
      Title: Role of accessory DNA polymerases in DNA replication in Escherichia coli: analysis of the dnaX36 mutator mutant.
    10. N-terminus verified by Edman degradation on mature peptide
      Title: DNA polymerase III holoenzyme of Escherichia coli. I. Purification and distinctive functions of subunits tau and gamma, the dnaZX gene products.
    Submit: New GeneRIF Correction See all GeneRIFs (13)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-directed DNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA replication NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in DNA-templated DNA replication IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA-templated DNA replication NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in viral translational frameshifting IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of DNA polymerase III complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of DNA polymerase III complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    part_of DNA polymerase III, clamp loader complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of replisome NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA polymerase III subunit gamma;DNA polymerase III subunit tau

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      492092..494023
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415003.1 DNA polymerase III subunit tau [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415003.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6N8QX54
      Conserved Domains (1) summary
      PRK07994
      Location:1 → 643
      PRK07994; DNA polymerase III subunits gamma and tau; Validated

    2. YP_009518751.1 DNA polymerase III subunit gamma [Escherichia coli str. K-12 substr. MG1655]

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8T6BMW8
      Conserved Domains (1) summary
      PRK07994
      Location:1 → 430
      PRK07994; DNA polymerase III subunits gamma and tau; Validated
    Nucleotide Protein
    Heading Accession and Version

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