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Status
Public on Aug 19, 2004
Title
UTSW Human 22k Oligo spotted array
Technology type
spotted oligonucleotide
Distribution
non-commercial
Organism
Homo sapiens
Description
Construction and Preparation of Human Oligo 22K Spotted Microarrays by UTSW: Operon Human Genome Oligo Set (Version 2) (70mers) representing 21,329 human genes in 150mM Sodium Phosphate (pH8.3) plus 1xSSC at 30 µM were spotted singly onto polymer-coated glass slides (Full Moon Biosystems) using BioRobotics MicroGrid II spotter (Genomic Solutions) fitted with 4x12 Microspot 2500 quill pins at 20-23°C and 60-65% RH. Spot are arranged with 22x22 spots per block (48 total blocks) and spot centers are 200 µm apart. Printed slides were subjected to a UV-cross-linking at a dose of 650mJ cm2 for probe immobilization. Slides with immobilized probes were then stored in slide holders and placed in a desiccator at room temperature.
Web link
http://microarray.swmed.edu Contributor(s)
Ayoubi P , Zhou A Submission date
Aug 17, 2004
Last update date
Oct 28, 2005
Contact name
Patricia J Ayoubi
E-mail(s)
[email protected] Phone
405-744-6209
Fax
405-744-7799
URL
http://microarray.okstate.edu Organization name
Oklahoma State University
Department
Biochemistry and Molecular Biology
Lab
OSU Microarray Core Facility
Street address
246 NRC
City
Stillwater
State/province
OK
ZIP/Postal code
74078
Country
USA
Samples (5)
GSM29237 , GSM29238 , GSM29239 , GSM29240 , GSM29241 Series (1)
GSE1694 Promyelocytic cells response to Anaplasma phagocytophilum infection
Data table header descriptions ID Spot Index Number
Block Spot block number
Column Spot column number
Row Spot row number
Probe_ID Probe ID
GB_ACC Genbank Accession Number
Description Descriptive gene name, from UniGene
Oligo_ID Operon oligo ID
UNIGENE UniGene cluster, human UniGene Build Hs 147, ID
Gene_Symbol Gene symbol, from UniGene
SPOT_ID Column added by GEO staff
Data table ID Block Column Row Probe_ID GB_ACC Description Oligo_ID UNIGENE Gene_Symbol SPOT_ID 1
1
1
1
control
buffer
control
2
1
2
1
control
buffer
control
3
1
3
1
NM_007216
NM_007216 Alpha integrin binding protein 63
H200003900
29205
KIAA1017
4
1
4
1
AK022156
AK022156 Homo sapiens cDNA FLJ12094 fis
H200003924
29438
5
1
5
1
NM_024613
NM_024613 Phafin 2
H200003948
29724
FLJ13187
6
1
6
1
NM_000112
NM_000112 Solute carrier family 26 (sulfate transporter)
H200003972
29981
SLC26A2
7
1
7
1
NM_002401
NM_002401 Mitogen-activated protein kinase kinase kinase 3
H200003906
29282
MAP3K3
8
1
8
1
BC015419
BC015419 Homo sapiens
H200003930
29497
9
1
9
1
NM_053043
NM_053043 Hypothetical protein MGC20460
H200003954
29796
MGC20460
10
1
10
1
NM_020662
NM_020662 MRS2-like
H200003978
30057
MRS2L
11
1
11
1
NM_006231
NM_006231 Polymerase (DNA directed)
H200007700
166846
POLE
12
1
12
1
NM_015896
NM_015896 BLu protein
H200007724
167380
BLu
13
1
13
1
NM_002302
NM_002302 Leukocyte cell-derived chemotaxin 2
H200007748
167877
LECT2
14
1
14
1
AL080134
AL080134 Homo sapiens mRNA
H200007772
168762
15
1
15
1
NM_005245
NM_005245 FAT tumor suppressor homolog 1 (Drosophila)
H200007706
166994
FAT
16
1
16
1
NM_021965
NM_021965 Phosphoglucomutase 5
H200007730
167473
PGM5
17
1
17
1
NM_004818
NM_004818 Prp28
H200007754
168103
U5-100K
18
1
18
1
U36759
U36759 Human pre TCR alpha mRNA
H200007778
169002
19
1
19
1
blank
blank
blank
20
1
20
1
blank
blank
blank
23232 1912 Kbytes .Supplementary data files not provided
