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| Status |
Public on Feb 25, 2005 |
| Title |
Promyelocytic cells response to Anaplasma phagocytophilum infection |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Gene expression profiling of human promyelocytic cells in response to infection with Anaplasma phagocytophilum.
Total RNA derived from 3DPI Anaplasma phagocytophilum-infected HL-60 cells was labeled with A647 and total RNA derived from 3DPI Mock-infected HL-60 cells was labeled with A546. For each, 5 µg of total RNA was labeled using Genisphere Array900, Alexa Fluor dyes and SuperscriptII. Slide scanned with ScanArray Express and images processed with GenePix Pro version 4.0. Normalized log ratios VALUES determined using R-project statistical environment (http://www.r-project.org) and Bioconductor (http://www.bioconductor.org) through the GenePix AutoProcessor (GPAP, http://darwin.biochem.okstate.edu/gpap) website (P. Ayoubi, unpublished results).
Keywords: time-course
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| Contributor(s) |
de la Fuente J, Ayoubi P, Blouin EF, Almazán C, Naranjo V, Kocan KM |
| Citation(s) |
15760455 |
| |
| Submission date |
Aug 21, 2004 |
| Last update date |
Mar 15, 2012 |
| Contact name |
Patricia J Ayoubi |
| E-mail(s) |
[email protected]
|
| Phone |
405-744-6209
|
| Fax |
405-744-7799
|
| URL |
http://microarray.okstate.edu
|
| Organization name |
Oklahoma State University
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
OSU Microarray Core Facility
|
| Street address |
246 NRC
|
| City |
Stillwater |
| State/province |
OK |
| ZIP/Postal code |
74078 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1411 |
UTSW Human 22k Oligo spotted array |
|
| Samples (5)
|
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| Relations |
| BioProject |
PRJNA90657 |
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