|
| Status |
Public on Dec 29, 2008 |
| Title |
Gastric Cancer Cell Lines |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by SNP array
Genome variation profiling by genome tiling array
|
| Summary |
Regions of recurrent genomic amplification and deletion are frequently observed in primary gastric cancers (GC). However, identifying specific oncogenes and tumor suppressor genes within these regions can be challenging, as they often cover tens to hundreds of genes. Here, we combined high-resolution array-based comparative genomic hybridization (aCGH) with gene expression profiling to target genes lying within focal high-level amplifications in GC cell lines, and identified RAB23 as an amplified and overexpressed Chr 6p11p12 gene in Hs746T cells. High RAB23 protein expression was also observed in some lines lacking RAB23 amplification, suggesting additional mechanisms besides gene amplification for up-regulating RAB23. siRNA silencing of RAB23 reduced the invasive potential of both amplified and nonamplified GC cell lines. RAB23 gene amplifications were observed in 13% of primary gastric carcinomas. In two independent patient cohorts, RAB23 transcript and protein expression was significantly associated with diffuse-type gastric cancer (dGC) compared to intestinal-type gastric cancer (iGC), providing further evidence that dGC and iGC likely represent two molecularly distinct tumor types. Our study demonstrates that investigating focal chromosomal amplifications by combining highresolution aCGH with expression profiling is a powerful general strategy for identifying novel cancer genes in recurrent regions of chromosomal aberration.
Keywords: gastric cancer cell lines, comparative genomic hybridization, gene expression profiling
|
| |
|
| Overall design |
Affy 100K SNP profiling and 32K BAC Array profiling for 7 Gastric Cancer Cell Lines
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| |
|
| Contributor(s) |
Wu Y, Tan P |
| Citation(s) |
18559507 |
| |
| Submission date |
Feb 22, 2008 |
| Last update date |
Dec 22, 2017 |
| Contact name |
Wu Yonghui |
| E-mail(s) |
[email protected]
|
| Organization name |
National Cancer Center of Singapore
|
| Street address |
11 Hospital Drive
|
| City |
Singapore |
| ZIP/Postal code |
169610 |
| Country |
Singapore |
| |
|
| Platforms (4)
|
| GPL2004 |
[Mapping50K_Hind240] Affymetrix Human Mapping 50K Hind240 SNP Array |
| GPL2005 |
[Mapping50K_Xba240] Affymetrix Human Mapping 50K Xba240 SNP Array |
| GPL6520 |
National Cancer Center of Singapore Human 32k BAC array(A) version 1 |
| GPL6521 |
National Cancer Center of Singapore Human 32k BAC array(B) version 1 |
|
| Samples (28)
|
|
| Relations |
| BioProject |
PRJNA107785 |
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