|
| Status |
Public on Dec 29, 2008 |
| Title |
Hs 746T Cell Line(32K_B) |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Hs 746T Cell Line(32K_B)
|
| Organism |
Homo sapiens |
| Characteristics |
Gastric Cell Line (Hs 746T)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Qiagen Blood and Cell Culture DNA extraction kit.
|
| Label |
Cy3
|
| Label protocol |
2 µg of cell line and reference genomic DNA (unrelated pooled male lymophocyte DNA from healthy donors) were labeled with Cy3-dCTP or Cy5-dCTP respectively (Amersham Biosciences) using the Bioprime Labelling system (Invitrogen)
|
| |
|
| Channel 2 |
| Source name |
reference genomic DNA
|
| Organism |
Homo sapiens |
| Characteristics |
unrelated pooled male lymophocyte DNA from healthy donors
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Qiagen Blood and Cell Culture DNA extraction kit.
|
| Label |
Cy5
|
| Label protocol |
2 µg of cell line and reference genomic DNA (unrelated pooled male lymophocyte DNA from healthy donors) were labeled with Cy3-dCTP or Cy5-dCTP respectively (Amersham Biosciences) using the Bioprime Labelling system (Invitrogen)
|
| |
|
| |
| Hybridization protocol |
Hybridizations were performed using a MAUI hybridization station, and hybridized onto microarrays containing 32,000 DOP-PCR amplified BAC clones covering the human genome (Children’s Hospital Oakland Research Institute, BACPAC resources)
|
| Scan protocol |
GeneChips were scanned using the GenePix 4000B scanner (Axon Instruments).
|
| Description |
Hs 746T Cell Line(32K_B)
aCGH profiling for Gastric Cell Lines
|
| Data processing |
The data were analyzed Genedata software for normalization and expression estimation
|
| |
|
| Submission date |
Feb 22, 2008 |
| Last update date |
Dec 29, 2008 |
| Contact name |
Wu Yonghui |
| E-mail(s) |
[email protected]
|
| Organization name |
National Cancer Center of Singapore
|
| Street address |
11 Hospital Drive
|
| City |
Singapore |
| ZIP/Postal code |
169610 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL6521 |
| Series (1) |
|
