|
| Status |
Public on Jun 21, 2008 |
| Title |
Global Transcriptional Response of Nitrosomonas europaea to Cyanide |
| Organism |
Nitrosomonas europaea |
| Experiment type |
Expression profiling by array
|
| Summary |
Pure cultures of ammonia oxidizing bacterium, Nitrosomonas europaea, are exposed to cyanide in pseudo-steady state batch reactor in presence of ammonia. Nitrosomonas europaea are generally regarded as the most sensitive organism to various inhibitors commonly encountered in the wastewater treatment plants (WWTP). To find stress genes of Nitrosomonas europaea to cyanide known as inhibitor of respiratory process, whole-genome transcript response to cyanide was determined in this research using microarray and qRT-PCR. When 1 uM NaCN inhibits nitrification about 50 %, transcript levels of 35 genes were increased while transcript levels of 29 genes were decreased, showing more than 2-fold in total 2460 genes. moeZ (NE2353), homologue with rhodanases related to detoxification of CN-, showed 7-fold up regulation and gene cluster including moeZ also showed significant up regulation.
Keywords: cyanide, stress response, moeZ
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| |
|
| Overall design |
The 1 uM NaCN caused more than 50 % inhibition in physiological response for 1 hour incubation. Transcriptional levels of the cells inhibited by cyanide were compared with the cells under control condition.
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| |
|
| Contributor(s) |
Park S, Ely RL |
| Citation(s) |
19072843 |
| |
| Submission date |
Feb 28, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Sunhwa Park |
| E-mail(s) |
[email protected]
|
| Phone |
541-737-6311
|
| Fax |
541-737-2082
|
| Organization name |
Oregon State University
|
| Department |
Biological & Ecological Eng.
|
| Lab |
Roger Ely's Lab
|
| Street address |
116 Gilmore
|
| City |
Corvallis |
| State/province |
OR |
| ZIP/Postal code |
97331 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL4617 |
Custom Nitrosomonas europaea GeneChip Array (Affymetrix) |
|
| Samples (6)
|
| GSM269650 |
Nitrosomonas europaea_control condition_60min_biological rep1 |
| GSM269651 |
Nitrosomonas europaea_control condition_60min_biological rep2 |
| GSM269652 |
Nitrosomonas europaea_control condition_60min_biological rep3 |
| GSM269653 |
Nitrosomonas europaea_exposure to 1 uM NaCN_60min_biological rep1 |
| GSM269654 |
Nitrosomonas europaea_exposure to 1 uM NaCN_60min_biological rep2 |
| GSM269655 |
Nitrosomonas europaea_exposure to 1 uM NaCN_60min_biological rep3 |
|
| Relations |
| BioProject |
PRJNA107633 |
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