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| Status |
Public on Aug 02, 2018 |
| Title |
Integrated regulation of apical hook development by transcriptional coupling of EIN3/EIL1 and PIFs in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
A dual-core processor, composed of EIN3/EIL1 and PIFs, mediates both positive and negative regulation of apical hook development by multiple hormones and light signals.
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| Overall design |
Three-day-old etiolated seedlings of Col-0, hls1, pifq, ein3 eil1, and pifq ein3 eil1 plants (not including roots) were collected and ground into a fine powder in liquid nitrogen. Two biological replicates were prepared for each genotype. Total RNA was extracted using the RNeasy Plant Mini kit (QIAGEN). The RNA sequencing and differential gene expression analysis were performed at the Bionova company. RNA quality was evaluated on a Bioanalyzer 2100 instrument (Agilent, Santa Clara, CA). Sequencing libraries were prepared following the protocol for the Directional RNA Library Prep Kit (NEB), and 150 nt paired-end high throughput sequencing was performed on an Illumina Hiseq X Ten system. Low quality sequencing reads were removed.
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| Contributor(s) |
Zhang X, Ji Y, Xue C, Ma H, Xi Y, Huang P, Wang H, An F, Li B, Wang Y, Guo H |
| Citation(s) |
30104405 |
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| Submission date |
Jul 09, 2018 |
| Last update date |
Dec 23, 2018 |
| Contact name |
xing zhang |
| E-mail(s) |
[email protected]
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| Organization name |
duke university
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| Street address |
FFSC Bldg., 124 Science Drive
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| City |
Durham |
| ZIP/Postal code |
27708 |
| Country |
USA |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA480268 |
| SRA |
SRP152847 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE116802_Hook_GEO_Processed_data_file.xls.gz |
2.7 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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