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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 06, 2019 |
| Title |
Expression data from WT, p110α-/-, p110δ-/-, and p110αδ double knockout HSC and LMPP hematopoietic populations |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Many cytokines and chemokines that are important in hematopoiesis activate the phosphatidylinositol 3-kinase kinase(PI3K) signaling pathway. Because this pathway is frequently mutated and activated in cancer, PI3K inhibitors have been developed for the treatment of several malignancies, and are now being tested in the clinic in combination with chemotherapy. However, the role of PI3K in adult hematopoietic stem cells (HSCs), particularly during hematopoietic stress, is still unclear.
To understand the molecular mechanisms of how p110α and p110δ regulate the HSC and progenitor stress response, we examined the effects of p110α and p110δ deletion on gene expression in hematopoietic stem cells (HSCs) and lymphoid-primed multipotential progenitors (LMPPs).
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| Overall design |
We sorted HSCs (Lin-ckit+Flk2-CD150+CD48-) and LMPPs (Lin-ckit+Flk2+ CD150-CD48+) from pooled bone marrow transplant recipients and performed DNA microarray analysis in triplicate using the Affymetrix Mouse Transcriptome Assay 1.0 kit.
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| Contributor(s) |
Hemmati S, Sinclair T, Tong M, Bartholdy B, Okabe RO, Ames K, Ostrodka L, Haque T, Kaur I, Mills TS, Agarwal A, Pietras EM, Zhao JJ, Roberts TM, Gritsman K |
| Citation(s) |
31120863 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 CA196973 |
PI3K Isoform Dependence in Adult Hematopoiesis and Myeloid Leukemia |
ALBERT EINSTEIN COLLEGE OF MEDICINE |
Kira Gritsman |
| K08 CA149208 |
The p110 alpha and delta isoforms of PI3 kinase in hematopoiesis and leukemia |
ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIVERSITY |
Kira Gritsman |
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| Submission date |
Aug 02, 2018 |
| Last update date |
Jun 06, 2019 |
| Contact name |
Shayda Hemmati |
| Organization name |
Albert Einstein College of Medicine
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| Street address |
1300 Morris Park
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| City |
Bronx |
| ZIP/Postal code |
10461 |
| Country |
USA |
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| Platforms (1) |
| GPL20775 |
[MTA-1_0] Affymetrix Mouse Transcriptome Array 1.0 [transcript (gene) CSV version] |
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| Samples (23)
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| Relations |
| BioProject |
PRJNA484198 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE118053_HSC_microarray_TAC_analysis.xlsx |
52.5 Kb |
(ftp)(http) |
XLSX |
| GSE118053_LMPP_microarray_TAC_analysis.xlsx |
112.0 Kb |
(ftp)(http) |
XLSX |
| GSE118053_RAW.tar |
598.3 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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