|
| Status |
Public on Jun 06, 2019 |
| Title |
p110αδ DKO HSC (KG2160-MTA1.22) |
| Sample type |
RNA |
| |
|
| Source name |
p110αδ DKO HSC
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
tissue: bone marrow
cell type: HSC
genotype: p110alphadelta DKO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation was performed using the (ARCTURUS PicoPure RNA Isolation Kit (Life Technologies, Invitrogen) according to the manufacturer’s protocol
[quantification protocol] RNA quantification was performed using the RNA Quantification Kit for SYBR Green I and ROX™ Passive Reference Dye (Thermo Fisher)
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
RNA was hybridized to the Gene Chip ® Mouse Transcriptome Pico Assay 1.0 using the GeneChip™ Hybridization, Wash, and Stain Kit
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
RMA expression value derived from Expression Console software
|
| Data processing |
Raw data were processed with expression console (Affymetrix) for probe set summarization and initial data quality examination. Data analysis and statistical evaluations were performed with Affymatrix Transcriptome analysis console (TAC) with 2 fold change and P value<0.05
|
| |
|
| Submission date |
Aug 02, 2018 |
| Last update date |
Jun 06, 2019 |
| Contact name |
Shayda Hemmati |
| Organization name |
Albert Einstein College of Medicine
|
| Street address |
1300 Morris Park
|
| City |
Bronx |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE118053 |
Expression data from WT, p110α-/-, p110δ-/-, and p110αδ double knockout HSC and LMPP hematopoietic populations |
|
