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| Status |
Public on Jul 01, 2019 |
| Title |
Expression data from Ricin-A expressing Drosophila wing discs |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Coordinated tissue growth is essential for generation of functionally integrated organs during animal development as well as for tissue homeostasis during adult life. Using Drosophila wing as a model system we have demonstrated that impairing growth, by expression of a cold sensitive version of type 2 ribosome-inactivating protein Ricin-A (RA), within defined territories along the wing primordium triggers a Dmp53-dependent reduction of growth in adjacent non-perturbed cells. To identify novel genes and pathways involved in coordinating intra-organ growth we analysed differential gene expression between wing discs expressing RA and control wing discs expressing GFP in the posterior compartment by using engrailed-Gal4.
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| Overall design |
Three independent RNA samples were prepared from wing imaginal discs of late L3 larvae expressing GFP or GFP plus RA under the control of en-gal4. After dissection of wing discs, the posterior GFP-positive compartment was separated under a fluorescence stereoscope and used for RNA extraction and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Mesquita D, Sanchez JA, Arce A, Milan M, Dekanty A |
| Citation(s) |
31425511 |
| |
| Submission date |
Jan 28, 2019 |
| Last update date |
Aug 20, 2019 |
| Contact name |
Andres Dekanty |
| E-mail(s) |
[email protected]
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| Phone |
543424511370
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| Organization name |
IAL CONICET
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| Street address |
COLECTORA RUTA 168
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| City |
SANTA FE |
| ZIP/Postal code |
3000 |
| Country |
Argentina |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA517523 |
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