|
| Status |
Public on Jul 01, 2019 |
| Title |
en>GFP, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Wing disc expressing GFP
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: Wing disc, posterior compartment
genotype: yw; enGal4,UAS-GFP
age: late L3 larvae
|
| Treatment protocol |
Drosophila wing imaginal discs were dissected and posterior GFP-positive compartment was separated under a fluorescence stereoscope
|
| Growth protocol |
Drosophila embryos expresing a cold sensitive version of Ricin-A (RA) under the control of en-gal4 were collected for 24 h and maintain at 18°C. Early second instar larvae were switched to 29ºC until late L3.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Library preparation and amplification were performed using Ovation RNA Amp System V (Nugen;3100-60). Fragmentation and labelling were performed using Encode Biotin Module (Nugen; 4200-60)
|
| |
|
| Hybridization protocol |
cDNA was hybridized to the microarray for 16 hours at 45ºC, washing and staining of microarrays was performed using a GeneChip Fluidics Station 450 (Affymetrix, Santa Clara, CA)
|
| Scan protocol |
Arrays scanning and CEL files generation were performed using an Affymetrix GeneChip Scanner GSC3000 (Affymetrix, Santa Clara, CA)
|
| Description |
Gene expression data from posterior wing disc cells of en-gal4, UAS-GFP larvae
|
| Data processing |
gcRMA normalization method as implemented in gcrma Bioconductor R package
|
| |
|
| Submission date |
Jan 28, 2019 |
| Last update date |
Jul 02, 2019 |
| Contact name |
Andres Dekanty |
| E-mail(s) |
[email protected]
|
| Phone |
543424511370
|
| Organization name |
IAL CONICET
|
| Street address |
COLECTORA RUTA 168
|
| City |
SANTA FE |
| ZIP/Postal code |
3000 |
| Country |
Argentina |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE125794 |
Expression data from Ricin-A expressing Drosophila wing discs |
|
