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| Status |
Public on Jul 16, 2019 |
| Title |
Small RNA sequencing reveals new mode of action and potential biomarkers for the mycotoxin Zearalenone |
| Organism |
Sus scrofa |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Purpose: The mycotoxin zearalenone (ZEN) poses a risk to animal health because of its estrogenic effects. Diagnosis of ZEN-induced disorders in animal production remains challenging due to the lack of appropriate non-invasive biomarkers. Thus, we combined untargeted and targeted transcriptomics approaches to investigate the effects of ZEN on the microRNA expression in porcine uterus, jejunum and serum.
Methods: twenty-four piglets received either uncontaminated feed (Control) or feed containing 0.17 mg/kg ZEN (ZEN low), 1.46 mg/kg ZEN (ZEN medium) and 4.58 mg/kg ZEN (ZEN high) for 28 days. microRNA from uterus and jejunum tissues were profiled by next-generation small RNA sequencing. Serum microRNA expression was analyzed by RT-qPCR analysis.
Results: the microRNA expression in the jejunum remained unaffected, while significant changes in the uterine microRNA profile were observed. Importantly, 14 microRNAs were commonly and in a dose-dependent manner affected in both the ZEN medium and ZEN high group, including microRNAs from the miR-503 cluster. Although the effects in the serum were less pronounced, the receiver operating characteristic analysis revealed that several microRNA ratios were able to discriminate properly between non-exposed and ZEN-exposed pigs (e.g. ssc-miR-135a-5p/ssc-miR-432-5p, ssc-miR-542-3p/ssc-miR-493-3p).
Conclusions: This work sheds some new light on the molecular mechanisms of ZEN, and fosters biomarker discovery.
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| Overall design |
Uterus and jejunum microRNA profiles at 28 days post weaned piglets. For each tissue, 6 biological replicates (= 6 pigs) from Control, 4 from ZEN low, 4 from ZEN medium, and 4 from ZEN high were used for library preparation. The final multiplexed (n=18) libraries were sequenced on a NextSeq 500
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| Contributor(s) |
Grenier B, Hackl M, Skalicky S, Thamhesl M, Moll WD, Berrios R, Schatzmayr G, Nagl V |
| Citation(s) |
31253833 |
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| Submission date |
Feb 25, 2019 |
| Last update date |
Jul 16, 2019 |
| Contact name |
Bertrand Grenier |
| E-mail(s) |
[email protected]
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| Organization name |
BIOMIN
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| Department |
Research Center
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| Street address |
Technopark 1
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| City |
Tulln |
| ZIP/Postal code |
3430 |
| Country |
Austria |
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| Platforms (1) |
| GPL20983 |
Illumina NextSeq 500 (Sus scrofa) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA524102 |
| SRA |
SRP186763 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE126989_data_ref_5307_Pool1_Uterus.xlsx |
2.1 Mb |
(ftp)(http) |
XLSX |
| GSE126989_data_ref_5307_Pool2_Jejunum.xlsx |
3.1 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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