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| Status |
Public on May 01, 2009 |
| Title |
Expression data from Pseudomonas aeruginosa exposed to a low concentration of Azithromycin |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by array
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| Summary |
Low-dose macrolides are effective therapy in patients with chronic lung infections, but the mechanisms of action are unclear. We compared global gene expression profiles between P.aeruginosa with and without low-dose Azithromycin (AZM) to study why the low-dose macrolide therapy is effective for cystic fibrosis and diffuse panbronchiolitis.
Keywords: dose-response
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| Overall design |
Overnight culture of P. aeruginosa strain PAO1 was inoculated at 1:100 into fresh LB medium. Various concentrations of AZM (0, 0.5, 2 ug/ml) were added to th culture media. Cells were collected 9 hours after the addition of AZM, and total RNA was isolated for DNA microarray experiments.
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| Contributor(s) |
Kai T, Tateda K, Kimura S, Ishii Y, Ito H, Yoshida H, Kimura T, Yamaguchi K |
| Citation(s) |
19393329 |
| |
| Submission date |
Sep 11, 2008 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Toshihiro Kai |
| E-mail(s) |
[email protected]
|
| Phone |
+81-6-6466-5211
|
| Organization name |
Dainippon Sumitomo Pharma Co., Ltd.
|
| Department |
Genomics Research
|
| Lab |
Genomic Science Laboratories
|
| Street address |
3-1-98 Kasugade-naka, Konohana-ku
|
| City |
Osaka |
| State/province |
Osaka |
| ZIP/Postal code |
554-0022 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL84 |
[Pae_G1a] Affymetrix Pseudomonas aeruginosa Array |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA111043 |
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