|
| Status |
Public on May 01, 2009 |
| Title |
AZM 2 ug/ml, replicate |
| Sample type |
RNA |
| |
|
| Source name |
P. aeruginosa, AZM 2 ug/ml, 9 hours
|
| Organism |
Pseudomonas aeruginosa |
| Characteristics |
Strain: PAO1
|
| Treatment protocol |
P. aeruginosa was grown in 15ml LB medium at 37°C shaking without or with AZM (Pfizer Laboratories) (0.5, 2 ug/ml).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
We added RNAprotect Bacteria Reagent (QIAGEN) to medium before extraction. Then, we extracted RNA with RNeasy Midi Kit (QIAGEN) according to the manufacturer's instructions. Finaly, we used RNase-Free DNase set (QIAGEN) to remove genomic DNA contamination.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures ( using Affymetrix GeneChip Hybridization Oven 640 and Affymetrix Fluidics Station 450)
|
| Scan protocol |
Standard Affymetrix procedures( using Hewlett-Packard GeneArray Scanner G2500A)
|
| Description |
2 ug/ml AZM is 1/64 MIC
|
| Data processing |
Data processing was carried out by GCOS1.1. MAS5.0 algorithm was used to generate signals and detection calls. Global normalization was performed to make average intensity of all probe sets equal to 100.
|
| |
|
| Submission date |
Sep 11, 2008 |
| Last update date |
Apr 16, 2009 |
| Contact name |
Toshihiro Kai |
| E-mail(s) |
[email protected]
|
| Phone |
+81-6-6466-5211
|
| Organization name |
Dainippon Sumitomo Pharma Co., Ltd.
|
| Department |
Genomics Research
|
| Lab |
Genomic Science Laboratories
|
| Street address |
3-1-98 Kasugade-naka, Konohana-ku
|
| City |
Osaka |
| State/province |
Osaka |
| ZIP/Postal code |
554-0022 |
| Country |
Japan |
| |
|
| Platform ID |
GPL84 |
| Series (1) |
| GSE12738 |
Expression data from Pseudomonas aeruginosa exposed to a low concentration of Azithromycin |
|
