|
| Status |
Public on Mar 27, 2019 |
| Title |
C19ORF66 broadly escapes viral-induced endonuclease cleavage and restricts Kaposi’s Sarcoma Associated Herpesvirus (KSHV) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the application of RNA-seq to identify the mRNA targets of 4 herpesviral endonucleases and uncovered a cluster of transcripts escaping degradation from all tested endonucleases.
|
| |
|
| Overall design |
mRNA profiles were obtained from HEK-293T cells that were transfected with 4 herpesviral endonuclease, cells sorted and total mRNA extracted, polyA enriched and after library prep were sequences on a HiSeq 4000
|
| |
|
| Contributor(s) |
Muller M, Rodriguez W |
| Citation(s) |
36914661 |
| |
| Submission date |
Mar 26, 2019 |
| Last update date |
Jul 14, 2023 |
| Contact name |
Mandy Muller |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Massachusetts, Amherst
|
| Department |
Microbiology
|
| Street address |
639 North Pleasant Street, 101 Morrill IVN
|
| City |
Amherst |
| State/province |
MA |
| ZIP/Postal code |
01003 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA529180 |
| SRA |
SRP189464 |
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