|
| Status |
Public on Mar 27, 2019 |
| Title |
BGLF5 replicate 2 |
| Sample type |
SRA |
| |
|
| Source name |
HEK-293T_BGLF5
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK-293T
genotype/variation: BGLF5
|
| Growth protocol |
293T cells (ATCC) were grown in DMEM (Invitrogen) supplemented with 10% FBS, routinely passaged and transfected using PolyJet
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction by Trizol
Poly A selection, RNA check on Bioanalyzer and standard library prep
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
8_S9_L002_R1_001
|
| Data processing |
Using Galaxy, reads were then aligned to the human genome (hg38) by Bowtie2
differential expression analysis were performed using Cufflink and Cuffdiff2
Genome_build: hg38
Supplementary_files_format_and_content: tab-delimited text files include average (over replicates) FPKM values for each Sample
|
| |
|
| Submission date |
Mar 26, 2019 |
| Last update date |
Mar 28, 2019 |
| Contact name |
Mandy Muller |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Massachusetts, Amherst
|
| Department |
Microbiology
|
| Street address |
639 North Pleasant Street, 101 Morrill IVN
|
| City |
Amherst |
| State/province |
MA |
| ZIP/Postal code |
01003 |
| Country |
USA |
| |
|
| Platform ID |
GPL20301 |
| Series (1) |
| GSE128866 |
C19ORF66 broadly escapes viral-induced endonuclease cleavage and restricts Kaposi’s Sarcoma Associated Herpesvirus (KSHV) |
|
| Relations |
| BioSample |
SAMN11258076 |
| SRA |
SRX5576733 |
