|
| Status |
Public on Oct 07, 2019 |
| Title |
Induction of human hemogenesis in adult fibroblasts by defined factors and hematopoietic co-culture |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Previous work demonstrates an induction of definitive hematopoiesis from human dermal fibroblasts (HDFs) after transduction with the transcription factors (TFs) GATA2, GFI1B, and FOS. To improve the yield and function of the derived hematopoietic cells, TF screening identified GFI1 as a causative factor in hematopoietic cell expansion with in vitro and in vivo functional capacity.
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| |
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| Overall design |
mRNAseq profiling of adult human dermal fibroblasts (HDFs) at the population level after transduction with GATA2, GFI1, GFI1B and FOS at days 15 and 25
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| |
|
| Contributor(s) |
Daniel MG, Sachs D, Moore KA |
| Citation(s) |
31557312 |
| |
| Submission date |
Apr 26, 2019 |
| Last update date |
Jan 06, 2020 |
| Contact name |
MICHAEL DANIEL |
| E-mail(s) |
[email protected]
|
| Organization name |
ICAHN SCHOOL OF MEDICINE OF MOUNT S
|
| Street address |
1 GUSTAVE L LEVY PL # 1496
|
| City |
NEW YORK |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (12)
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|
| Relations |
| BioProject |
PRJNA539895 |
| SRA |
SRP193952 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE130361_D15_3GF_CD49f_vs_D15_3GF_CD34_CD49f.csv.gz |
44.6 Kb |
(ftp)(http) |
CSV |
| GSE130361_D25_3GF_CD34_CD49f_vs_D15_3GF_CD34_CD49f.csv.gz |
21.8 Kb |
(ftp)(http) |
CSV |
| GSE130361_D25_3GF_CD34_CD49f_vs_D15_3GF_CD49f.csv.gz |
56.8 Kb |
(ftp)(http) |
CSV |
| GSE130361_D25_3GF_CD49f_vs_D15_3GF_CD34_CD49f.csv.gz |
245.4 Kb |
(ftp)(http) |
CSV |
| GSE130361_D25_3GF_CD49f_vs_D15_3GF_CD49f.csv.gz |
289.5 Kb |
(ftp)(http) |
CSV |
| GSE130361_D25_3GF_CD49f_vs_D25_3GF_CD34_CD49f.csv.gz |
163.8 Kb |
(ftp)(http) |
CSV |
| GSE130361_DESeq2_Normalized_Read_Counts.csv.gz |
1.3 Mb |
(ftp)(http) |
CSV |
| GSE130361_GGF_D15_CD49f_vs_3GF_D15_CD49f_TF_comparisons.xlsx |
885.5 Kb |
(ftp)(http) |
XLSX |
| GSE130361_GGF_D25_CD49fCD34_vs_3GF_D25_CD49f_CD34_TF_comparisons.xlsx |
857.8 Kb |
(ftp)(http) |
XLSX |
| GSE130361_GGF_D25_CD49f_vs_3GF_D25_CD49f_TF_comparisons.xlsx |
848.8 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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