|
| Status |
Public on Oct 07, 2019 |
| Title |
D25_3GF_CD49f+_CD34-_3 |
| Sample type |
SRA |
| |
|
| Source name |
adult human dermal fibroblast
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HDF
cell type: adult human dermal fibroblast
genotype: wild type
cell markers: CD49f+CD34-
time after transduction: day 25
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were sorted by FACS and bulk RNA was then isolated using the NucleoSpin RNA XS extraction kit
Illumina Nextera XT DNA Library Preparation Kit was used with 0.1-0.3ng of cDNA for the construction of sequencing libraries
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Alignment with RNA Star
Gene counting with featureCounts
Normalization and differential gene expression with DESeq2
Pathway analysis with GAGA
Genome_build: GRch37.75
Supplementary_files_format_and_content: Comparative analyses compiled with Biojupies or R; read counts derived from DESeq2
|
| |
|
| Submission date |
Apr 26, 2019 |
| Last update date |
Oct 07, 2019 |
| Contact name |
MICHAEL DANIEL |
| E-mail(s) |
[email protected]
|
| Organization name |
ICAHN SCHOOL OF MEDICINE OF MOUNT S
|
| Street address |
1 GUSTAVE L LEVY PL # 1496
|
| City |
NEW YORK |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE130361 |
Induction of human hemogenesis in adult fibroblasts by defined factors and hematopoietic co-culture |
|
| Relations |
| BioSample |
SAMN11514611 |
| SRA |
SRX5741684 |
