Monoclonal cell line L29 (derived from HEK-293 parental cell culture) with a low level of store-operated Ca2+ entry (SOCE). For selection of clones, HEK-293 cells were loaded with indo-1 and sorted by FACS on the basis of their cyclopiazonic acid (CPA)-stimulated Ca2+ entry. Monoclonal cell lines were selected from the sorted cells and their levels of SOCE confirmed by monitoring thapsigargin-stimulated Ba2+ entry. Total RNA was extracted from cells immediately after removal from their growth environment. RNA was processed and hybridized to the Affymetrix HG-U133A chip. Keywords = cDNA microarray Keywords = FACS analysis Keywords = thapsigargin Keywords = store-operated Ca2+ entry (SOCE)
