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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 16, 2009 |
| Title |
Expression profiles of beta-hematin (BH)- or 4-hydroxy-2-nonenal (HNE)-treated RAW 264.7 cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Hemozoin phagocytosis results in immunomodulation. In the present study, gene expression profiles were analyzed to identify transcriptional changes associated with two of the individual components of Hz in a model macrophage cell line. LPS-stimulated RAW 264.7 cells were exposed to the synthetic form of Hz, β-hematin (BH, 0.1 mg/mL) and HNE (35 μM) for 6 or 24 h.
Keywords: variable treatment
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| Overall design |
Three biological replicates were analyzed for each 3 treatment types (HNE, BH, bead) in LPS stimulated cells in addition to 3 replicates of untreated LPS stimulated cells (control). Each replicate was composed of 6 pooled wells from a 6-well plate. In order to identify transcriptional changes dependent on interactions of BH rather than those due to phagocytosis, differentially expressed mRNAs were controlled by a corresponding particulate latex bead challenge.
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| Contributor(s) |
Schrimpe AC, Wright DW |
| Citation(s) |
19191707 |
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| Submission date |
Oct 20, 2008 |
| Last update date |
Mar 04, 2019 |
| Contact name |
David W Wright |
| E-mail(s) |
[email protected]
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| Phone |
615-322-2636
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| Fax |
615-343-1234
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| Organization name |
Vanderbilt University
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| Department |
Chemistry
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| Street address |
SC Stevenson Center 7, Station B 351822
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| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37235-1822 |
| Country |
USA |
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| Platforms (2) |
| GPL2995 |
ABI Mouse Genome Survey Microarray |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (24)
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| GSM290639 |
BH, biological rep1 |
| GSM290640 |
BH, biological rep2 |
| GSM290641 |
BH, biological rep3 |
| GSM290643 |
HNE, biological rep1 |
| GSM290644 |
HNE, biological rep2 |
| GSM290645 |
HNE, biological rep3 |
| GSM290646 |
LPS, biological rep1 |
| GSM290647 |
LPS, biological rep2 |
| GSM290648 |
LPS, biological rep3 |
| GSM335277 |
6 h LPS, biological rep1 |
| GSM335278 |
6 h LPS, biological rep2 |
| GSM335279 |
6 h HNE+LPS, biological rep1 |
| GSM335280 |
6 h HNE+LPS, biological rep2 |
| GSM335281 |
6 h HNE+LPS, biological rep3 |
| GSM335282 |
6 h BH+LPS, biological rep1 |
| GSM335283 |
6 h BH+LPS, biological rep2 |
| GSM335284 |
6 h BH+LPS, biological rep3 |
| GSM335285 |
6 h bead+LPS, biological rep1 |
| GSM335286 |
6 h bead+LPS, biological rep2 |
| GSM335287 |
6 h bead+LPS, biological rep3 |
| GSM335946 |
6 h LPS, biological rep3 |
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| Relations |
| BioProject |
PRJNA109601 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE13281_RAW.tar |
47.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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