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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 05, 2019 |
| Title |
The MYCL and MXD1 transcription factors regulate the fitness of murine dendritic cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The present study reveals LMYC and MXD1 as novel regulators of a transcriptional program that is modulated during the maturation of Batf3-dependent dendritic cells (also known as type I classical dendritic cells or cDC1s).
We used microarray analysis with ERCC spike in controls to determine the transcriptional effects of MYCL and MXD1 deficiency at steady state and after activation with poly IC. Mycl-deficient mice are available from Jackson laboratories as B6.129S6(C)-Mycltm1.1Kmm/J. Mxd1-/- mice were provided by R. Eisenman.
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| Overall design |
Splenic cDC1s were isolated from wild type and Mycl-deficient mice treated with or without polyIC for 5 hours, or wild type or Mxd1-deficient mice treated with poly IC for 18 hours. Total RNA was isolated from FACS-purified cDC1s, ERCC spike in controls were added by normalizing to cell count, and RNA was amplified by GeneChip WT Pico Kit. Samples were in turn analyzed by expression microarray analysis on
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| Contributor(s) |
Anderson III DA, Murphy KM |
| Citation(s) |
32071205 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| F31 CA228240 |
The regulation of dendritic cell function by LMYC at steady state and during immune responses to cancer |
WASHINGTON UNIVERSITY |
David Alexander Anderson |
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| Submission date |
Dec 04, 2019 |
| Last update date |
Mar 23, 2020 |
| Contact name |
David Alexander Anderson III |
| E-mail(s) |
[email protected]
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| Organization name |
Washington University School of Medicine
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| Department |
Pathology and Immunology
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| Lab |
Murphy
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| Street address |
660. S Euclid Ave., Campus Box 8118
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| City |
St. Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (35)
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| GSM4204767 |
Steady State, LMYC HET1, Biological Rep 1 |
| GSM4204768 |
Steady State, LMYC KO1, Biological Rep 1 |
| GSM4204769 |
Steady State, LMYC KO1, Biological Rep 2 |
| GSM4204770 |
Steady State, LMYC KO1, Biological Rep 3 |
| GSM4204771 |
Steady State, LMYC KO1, Biological Rep 4 |
| GSM4204772 |
Steady State, LMYC HET1, Biological Rep 2 |
| GSM4204773 |
Steady State, LMYC HET1, Biological Rep 3 |
| GSM4204774 |
Steady State, LMYC HET1, Biological Rep 4 |
| GSM4204775 |
Steady State, LMYC HET1, Biological Rep 5 |
| GSM4204776 |
Steady State, LMYC KO2, Biological Rep 1 |
| GSM4204777 |
Steady State, LMYC KO2, Biological Rep 2 |
| GSM4204778 |
Steady State, LMYC KO2, Biological Rep 3 |
| GSM4204779 |
Steady State, LMYC KO2, Biological Rep 4 |
| GSM4204780 |
Steady State, LMYC KO2, Biological Rep 5 |
| GSM4204781 |
Steady State, LMYC WT2, Biological Rep 1 |
| GSM4204782 |
Steady State, LMYC WT2, Biological Rep 2 |
| GSM4204783 |
Steady State, LMYC WT2, Biological Rep 3 |
| GSM4204784 |
Steady State, LMYC WT2, Biological Rep 4 |
| GSM4204785 |
Steady State, LMYC WT2, Biological Rep 5 |
| GSM4204786 |
poly IC Activated, LMYC WT3, Biological Rep 1 |
| GSM4204787 |
poly IC Activated, LMYC WT3, Biological Rep 2 |
| GSM4204788 |
poly IC Activated, LMYC WT3, Biological Rep 3 |
| GSM4204789 |
poly IC Activated, LMYC WT3, Biological Rep 4 |
| GSM4204790 |
poly IC Activated, LMYC WT3, Biological Rep 5 |
| GSM4204791 |
poly IC Activated, LMYC KO3, Biological Rep 1 |
| GSM4204792 |
poly IC Activated, LMYC KO3, Biological Rep 2 |
| GSM4204793 |
poly IC Activated, LMYC KO3, Biological Rep 3 |
| GSM4204794 |
poly IC Activated, LMYC KO3, Biological Rep 4 |
| GSM4204795 |
poly IC Activated, LMYC KO3, Biological Rep 5 |
| GSM4204796 |
poly IC Activated, MXD1 WT1, Biological Rep 1 |
| GSM4204797 |
poly IC Activated, MXD1 WT1, Biological Rep 2 |
| GSM4204798 |
poly IC Activated, MXD1 WT1, Biological Rep 3 |
| GSM4204799 |
poly IC Activated, MXD1 KO1, Biological Rep 1 |
| GSM4204800 |
poly IC Activated, MXD1 KO1, Biological Rep 2 |
| GSM4204801 |
poly IC Activated, MXD1 KO1, Biological Rep 3 |
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| Relations |
| BioProject |
PRJNA593609 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE141492_RAW.tar |
148.9 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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