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| Status |
Public on Jan 15, 2009 |
| Title |
Gene expression profiling of interferon-beta stimulated cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Cytoplasmic DNA triggers the activation of the innate immune system. While downstream signaling components have been characterized, the DNA sensing components remain largely elusive. We performed a systematic proteomics screen for proteins that associate with DNA, traversed to a screen for IFN-β-induced transcripts. We identified DSIRE (DNA sensor for the IL-1β response, previously called AIM2) as a candidate cytoplasmic sensor. DSIRE showed a marked selectivity for double-stranded DNA. DSIRE can recruit the inflammasome adaptor ASC and gets redistributed to ASC speckles upon coexpression of ASC. RNAi-mediated reduction of DSIRE expression led to an impairment in IL-1β maturation. Reconstitution of unresponsive cells with DSIRE, ASC, caspase 1 and IL-1β showed that DSIRE is sufficient for inflammasome activation. Overall, our data strongly suggest that DSIRE is a cytoplasmic DNA sensor for the inflammasome.
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|
| Overall design |
In a genomic screen, we used NIH3T3, L929 and RAW264.7 cells to identify genes that were transcriptionally regulated by IFN-β. We stimulated the cells with IFN-β for 4h, isolated the RNA and analyzed global changes in gene expression by microarray analysis. Overall, 225 genes were upregulated at least 3fold, among which numerous well-known interferon-induced genes were found
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| Contributor(s) |
Buerckstuemmer T, Bilban M, Superti-Furga G |
| Citation(s) |
19158679 |
| |
| Submission date |
Jan 14, 2009 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Martin Bilban |
| E-mail(s) |
[email protected]
|
| Phone |
++43 (0)1 40400 6441
|
| Organization name |
Medical University of Vienna
|
| Department |
|
| Street address |
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA111687 |
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