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| Status |
Public on Mar 11, 2020 |
| Title |
Gene expression data from splenocytes of 3 week-old offspring after maternal exposure to benzyl butyl phthalate (BBP) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The prenatal and early postnatal period is highly sensitive to environmental exposures that may interfere with the developmental programming of the immune system leading to an altered disease risk in later life. To clarify the role of early influences in activation or exacerbation of autoimmune diseases like rheumatoid arthritis (RA) we investigated the effect of maternal exposure during the prenatal and weaning period of DBA/1 mice to the plasticizer benzyl butyl phthalate (BBP) on the development of RA in the offspring. Using a mild collagen-induced arthritis (CIA) model, maternal BBP-exposure increased both the prevalence and the severity of RA in the progeny compared to un-exposed dams. Additionally, maternal BBP exposure led to elevated serum IgG1 and IgG2a level in the offspring and increased the IFN-? and IL-17 release from collagen-re-stimulated spleen cells. Transcriptome analysis of splenoytes isolated from 3-week-old pups before RA-induction revealed considerable changes in gene expression in the offspring from BBP-exposed dams. Among them were regulator of G-protein signaling 1 (rgs1), interleukin-7 receptor (il-7r) and CXC chemokine 4 (cxcr4), all genes that have previously been described as associated with RA pathology. In summary, our results demonstrate that perinatal exposure to BBP increases the susceptibility of the offspring to RA, probably via a phthalate-induced disturbed regulation of RA-relevant genes or signalling pathways.
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| Overall design |
Perinatal phthalate exposure increases the susceptibility to rheumatoid arthritis in mice. After perinatal exposure of mothers, splenocytes of 3-week old offsprings were pooled and analyzed.
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| Contributor(s) |
Polte T |
| Citation(s) |
32308655 |
| |
| Submission date |
Mar 10, 2020 |
| Last update date |
Apr 27, 2020 |
| Contact name |
Tobias Polte |
| E-mail(s) |
[email protected]
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| Organization name |
Helmholtz Centre for Environmental Research GmbH - UFZ
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| Street address |
Permoserstrasse 15
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| City |
Leipzig |
| ZIP/Postal code |
04318 |
| Country |
Germany |
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| Platforms (1) |
| GPL20775 |
[MTA-1_0] Affymetrix Mouse Transcriptome Array 1.0 [transcript (gene) CSV version] |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA611697 |
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