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| Status |
Public on Apr 17, 2020 |
| Title |
Expression data from Cyanidine (Cyanidine 3-glucoside)-treated human amnion epithelial cells (hAECs) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression profiling of the effect of Cyanidine 3 glucoside treatment in hAECs.
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| Overall design |
hAECs were isolated from discarded term placenta and were treated with 20 μM Cyanidine 3-glucoside for seven days. Microarray gene expression profiling was conducted for biological replicates of Cyanidine-treated and untreated control cells on day 7.
RNA was extracted using Isogen (Nippon Gene Co. Ltd., Toyama, Japan). The integrity of RNA was quantified using NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). RNA samples were prepared for gene expression profiling analysis with GeneChip® 3' Expression Arrays using 3' IVT PLUS Reagent Kit (Affymetrix Inc., Santa Clara, CA, USA). Two hundred and fifty ng of total RNA from each sample was used to generate amplified and biotinylated complementary RNA (cRNA) from poly (A) RNA in a total RNA sample according to the user manual. IVT Incubation time was 16 hour. GeneAtlas® Hybridization, Wash and Stain Kit was used for hybridizing 3' IVT Array Strips according to the user manual (P/N 08-0306). Human genome array strips (HG-U219) were hybridized for 16 hours in a 45oC incubator, washed and stained and finally imaging was done with the GeneAtlas Fluidics and Imaging Station.
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| Citation(s) |
33916494, 35433672 |
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| Submission date |
Apr 16, 2020 |
| Last update date |
Apr 20, 2022 |
| Contact name |
Farhana Ferdousi |
| E-mail(s) |
[email protected]
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| Organization name |
University of Tsukuba
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| Street address |
Tennodai 1-1-1
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| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8575 |
| Country |
Japan |
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| Platforms (1) |
| GPL13667 |
[HG-U219] Affymetrix Human Genome U219 Array |
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| Samples (4)
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| GSM4478763 |
Cyanidine 1: hAECs_Day 7_Cyanidine 1 |
| GSM4478764 |
Cyanidine 2: hAECs_Day 7_Cyanidine 2 |
| GSM4478765 |
Control 1: hAECs_Day 7_placental basal medium_Control 1 |
| GSM4478766 |
Control 2: hAECs_Day 7_placental basal medium_Control 2 |
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| Relations |
| BioProject |
PRJNA625697 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE148776_RAW.tar |
9.5 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| GSE148776_processed_data.xlsx |
5.8 Mb |
(ftp)(http) |
XLSX |
| Processed data included within Sample table |
| Processed data are available on Series record |
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