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Status
Public on Dec 16, 2004
Title
Fob3b vs F-line
Organism
Mus musculus
Experiment type
Expression profiling by array
Summary
Liver and BAT expression differences between the fat F-line, and congenic Fob3b-line. Normalised data appended as follows:
TABLE 1: LIVER - 1st set (7.5K) of the 2 slide NIA NIH 15K set -source1 = F-line liver male - 2 pools each made from 5 different individuals -source2 = Fob3b-line (aka Fchr15D-line) liver male - 2 pools each made from 5 different individuals -values are normalised using mixed model across 6 slides: 1 F-line vs Fline 2 Fob3b-line vs Fob3b-line 3 F-line pool1 vs Fob3b-line pool1 4 F-line pool1 vs Fob3b-line pool1 -dye swap 5 F-line pool2 vs Fob3b-line pool2 6 F-line pool2 vs Fob3b-line pool2 -dye swap
TABLE 2: LIVER - 2nd set (7.5K) of the 2 slide NIA NIH 15K set -source1 = F-line liver male - 2 pools each made from 5 differnt individuals -source2 = Fob3b-line (aka Fchr15D-line) liver male - 2 pools each made from 5 differnt individuals -values are normalised using mixed model across 6 slides: 1 F-line Vs F-line 2 Fob3b-line vs Fob3b-line 3 F-line pool1 vs Fob3b-line pool2 4 F-line pool1 vs Fob3b-line pool2 dye swap 5 F-line pool2 vs Fob3b-line pool1 6 F-line pool2 vs Fob3b-line pool1 dye swap
TABLE 3: Brown Adipose Tissue (BAT) 1st (7.5K) array set of 15K NIA NIH set -source1 = F-line male BAT from 2 pools each made from 5 different individuals -source2 = Fob3b-line (aka Fchr15D-line) male BAT from 2 pools each made from 5 different individuals -values are normalised using mixed model across 4 slides: 1 F-line pool1 vs Fob3b-line pool1 2 F-line pool1 vs Fob3b-line pool1 dye swap 3 F-line pool2 vs Fob3b-line pool2 4 F-line pool2 vs Fob3b-line pool2 dye swap
TABLE 4: Brown Adipose Tissue (BAT) 2nd (7.5K) array of the 15K NIA NIH set -source1 = F-line male BAT (2 pools each from 5 individual mice) -source2 = Fob3b-line (aka Fchr15D-line) male BAT (2 pools each from 5 individual mice) -values are normalised using mixed model across 4 slides: 1 F-line pool1 vs Fob3b-line pool1 2 F-line pool1 vs Fob3b-line pool1 dye swap 3 F-line pool2 vs Fob3b-line pool2 4 F-line pool2 vs Fob3b-line pool2 dye swap Keywords = Obesity Keywords = Fob3b Keywords = QTL Keywords = congenic Keywords: other
Contributor(s)
Stylianou IM , Clinton M , Pritchard C , Lallane Z , Bünger L , Bulfield G , Horvat S
Citation(s)
15598878
Submission date
Jul 12, 2004
Last update date
Mar 15, 2012
Contact name
Ioannis M Stylianou
E-mail(s)
[email protected]
Phone
001 (207) 288 6390
Fax
001 (207) 288 6078
Organization name
The Jackson Laboratory
Street address
600 Maine Street
City
Bar Harbor
State/province
ME
ZIP/Postal code
04609
Country
USA
Platforms (2)
GPL1341
1st 7.5K of NIA-NIH 15K Mouse cDNA set
GPL1342
2nd 7.5K of NIA-NIH 15K Mouse cDNA set
Samples (20)
Relations
BioProject
PRJNA90871
TABLE 1: LIVER - 1st set (7.5K) of the 2 slide NIA NIH 15K set header descriptions
CLONE_ID
IMAGE clone ID
VALUE
Log2 Fold change (-ve indicates upreg in F-line) Calculated using mixed model across 6 slides - each EST spotted twice
StdErr
Standard error
DF
Degrees of Freedom
tValue
tValue
Probt
Probablity
Data table
CLONE_ID
VALUE
StdErr
DF
tValue
Probt
H3001A08
-0.292894911
1.563236261
14
-0.187364456
0.854063174
H3001B12
0.798743227
1.197380432
12
0.667075564
0.517345971
H3001C09
0.414277146
1.355866032
16
0.305544306
0.763890935
H3001F04
-0.072419215
1.511603419
13
-0.047908872
0.962517073
H3001G03
-0.774746068
0.522724933
16
-1.482129548
0.157732768
H3001G05
0.432763228
0.723066792
14
0.598510722
0.559058906
H3001H01
-0.293848231
1.245398327
16
-0.235947186
0.8164674
H3001H07
1.112231608
0.942379222
14
1.18023783
0.257571091
H3002A06
-0.121397931
1.127152988
14
-0.107703153
0.915759461
H3002A11
0.16064651
1.16274849
16
0.138161014
0.89183703
H3002A12
-0.190121504
1.291101791
16
-0.14725524
0.884770161
H3002F08
-0.609606255
0.91800112
16
-0.664058291
0.51610912
H3002F12
-0.951535354
0.866020378
16
-1.098744762
0.288138539
H3002G05
1.139091272
1.181306653
14
0.964263825
0.351275224
H3002H05
0.922731448
0.867728305
16
1.063387517
0.303392919
H3002H07
-0.112758923
0.94774079
16
-0.118976543
0.906774936
H3003B02
0.093749582
0.106703384
16
0.878599899
0.392621867
H3003C08
0.446605321
0.251501195
16
1.775758245
0.094792472
H3003D08
-0.437418127
0.737834783
16
-0.592840209
0.561574586
H3003D09
-0.488988903
0.423273683
16
-1.155254679
0.264947862
Total number of rows: 7612 Table truncated, full table size 449 Kbytes .TABLE 2: LIVER - 2nd set (7.5K) of the 2 slide NIA NIH 15K set header descriptions
CLONE_ID
IMAGE clone ID
VALUE
Log2 Fold change (-ve indicate upreg in F-line)
StdErr
Standard Error
DF
Degrees of Freedom
tValue
tValue
Probt
Probability (untransformed P-Value from mixed model analysis)
Data table
CLONE_ID
VALUE
StdErr
DF
tValue
Probt
H3081A01
0.004664117
0.145508717
22
0.032053866
0.974718126
H3081A02
-0.072320977
0.134027296
22
-0.539598864
0.594893652
H3081A03
-0.114372989
0.17031988
22
-0.671518727
0.508880634
H3081A04
-0.133994372
0.121468133
22
-1.103123666
0.281894504
H3081A05
-0.017865375
0.063534883
22
-0.28119002
0.781192372
H3081A06
-0.020627802
0.112168728
22
-0.183899759
0.855776587
H3081A07
-0.210079119
0.153617177
22
-1.367549668
0.185262727
H3081A08
-0.172466974
0.160422231
22
-1.075081507
0.293988077
H3081A09
-0.125106514
0.236753453
22
-0.528425301
0.602494716
H3081A10
0.005891626
0.117296004
22
0.050228702
0.96039339
H3081A11
-0.06687604
0.096435614
22
-0.693478658
0.495266457
H3081A12
-0.200550766
0.209036041
22
-0.959407599
0.347779653
H3081B01
-0.385920422
0.169179022
22
-2.281136376
0.032580438
H3081B02
-0.037474376
0.092391448
22
-0.40560438
0.688950964
H3081B03
0.036821347
0.059547429
22
0.618353257
0.542692053
H3081B04
0.014632944
0.097279923
22
0.150421009
0.881803093
H3081B05
0.061599806
0.104229249
22
0.591003071
0.560541927
H3081B06
0.027868183
0.17988187
22
0.154924912
0.878293098
H3081B07
-0.346492883
0.148212899
22
-2.337805184
0.028895948
H3081B08
0.010258514
0.12552088
22
0.081727554
0.935602364
Total number of rows: 7567 Table truncated, full table size 447 Kbytes .TABLE 3: Brown Adipose Tissue (BAT) 1st (7.5K) array set of 15K NIA NIH set header descriptions
CLONE_ID
IMAGE clone ID
VALUE
Log2 Fold change (-ve indicates upreg in F-line)
StdErr
Standard Error
DF
Degrees of Freedom
tValue
tValue
Probt
Untransformed P-value from mixed model analysis
Data table
CLONE_ID
VALUE
StdErr
DF
tValue
Probt
H3001A01
0.456315918
0.180529603
10
2.527651484
0.029991401
H3001A02
-0.701835737
0.762029521
10
-0.921008593
0.378729137
H3001A03
-0.077784624
0.892686167
10
-0.087135464
0.932283971
H3001A04
-3.274976563
1.192417317
10
-2.746502014
0.020601557
H3001A05
-1.527301291
1.461584367
10
-1.044962799
0.320641986
H3001A06
-0.212039025
1.098174952
10
-0.193083101
0.850759087
H3001A07
0.317787558
0.558669102
10
0.568829665
0.582020846
H3001A08
-0.602955728
0.729100998
10
-0.826985192
0.427537841
H3001A09
0.737148351
1.015235428
10
0.726086118
0.484433351
H3001A10
0.357529777
0.252452352
10
1.416226761
0.187095855
H3001A11
0.323309378
0.515813031
10
0.626795678
0.544836179
H3001A12
-0.204401401
0.977955633
10
-0.209008869
0.838637458
H3001B01
-0.627122835
0.897535107
10
-0.698716775
0.500655538
H3001B02
-0.061511021
0.148102298
10
-0.415327932
0.686672834
H3001B03
-0.401489391
1.069937158
10
-0.375245769
0.715314122
H3001B04
-1.261052767
0.650977934
10
-1.937166685
0.081462298
H3001B05
-0.682135674
0.274630026
10
-2.483835011
0.032329789
H3001B06
-0.512604656
0.200965117
10
-2.550714588
0.028828608
H3001B07
1.281536303
0.672377659
10
1.905976924
0.085772561
H3001B08
-0.732200101
0.341344339
10
-2.14504832
0.057543069
Total number of rows: 7680 Table truncated, full table size 454 Kbytes .TABLE 4: Brown Adipose Tissue (BAT) 2nd (7.5K) array of the 15K NIA NIH set header descriptions
CLONE_ID
IMAGE clone ID
VALUE
Log2 Fold Change (-ve indicates upreg in F-line)
StdErr
Standard Error
DF
Degrees of Freedom
tValue
tValue
Probt
Untransformed P-value
Data table
CLONE_ID
VALUE
StdErr
DF
tValue
Probt
H3081A01
0.316498785
0.31031272
4
1.019934941
0.365427472
H3081A02
0.4578046
0.181512716
4
2.522162681
0.065206375
H3081A03
0.320735928
0.104566532
4
3.067290482
0.037391597
H3081A04
-0.031590701
0.038499014
4
-0.820558687
0.457982169
H3081A05
0.089408637
0.040036892
4
2.23315625
0.089297441
H3081A06
0.409037921
0.214930959
4
1.903113088
0.129772089
H3081A07
0.471586606
0.148509118
4
3.175472401
0.033680813
H3081A08
0.189821502
0.113805946
4
1.667940108
0.170653381
H3081A09
0.144489528
0.122642841
4
1.178132595
0.304047017
H3081A10
0.065777452
0.020028375
4
3.284213187
0.030380182
H3081A11
0.115518202
0.080202276
4
1.440335709
0.223198629
H3081A12
-0.040367333
0.223920113
4
-0.180275603
0.865700997
H3081B01
0.138911298
0.145587075
4
0.954145811
0.394041292
H3081B02
0.178051316
0.199024107
4
0.894621854
0.42155571
H3081B03
0.102446381
0.424551868
4
0.241304746
0.821183819
H3081B04
0.10888012
0.068199091
4
1.596503971
0.185612019
H3081B05
0.367633836
0.157314788
4
2.336931195
0.079645342
H3081B06
0.39752
0.417138579
4
0.952968677
0.394570339
H3081B07
0.646271738
0.280156962
4
2.306820198
0.0823194
H3081B08
0.249253101
0.149903597
4
1.662755968
0.171695397
Total number of rows: 7567 Table truncated, full table size 438 Kbytes .
Supplementary data files not provided