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| Status |
Public on Aug 18, 2020 |
| Title |
Comparative transcriptome analysis of human skeletal muscle in response to cold acclimation and exercise training in human volunteers. [A294] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Background: Cold acclimation and exercise training were previously shown to increase peripheral insulin sensitivity in human volunteers with type 2 diabetes. Although cold is a potent activator of brown adipose tissue, the increase in peripheral insulin sensitivity by cold is largely mediated by events occurring in skeletal muscle and at least partly involves GLUT4 translocation, as is also observed for exercise training. Results: To investigate if cold acclimation and exercise training overlap in the molecular adaptive response in skeletal muscle, we performed transcriptomics analysis on vastus lateralis muscle collected from human subjects before and after 10 days of cold acclimation, as well as before and after a 12-week exercise training intervention. Methods: Cold acclimation altered the expression of 756 genes (422 up, 334 down, P<0.01), while exercise training altered the expression of 665 genes (444 up, 221 down, P<0.01). Principal Component Analysis, Venn diagram, similarity analysis and Rank–rank Hypergeometric Overlap all indicated significant overlap between cold acclimation and exercise training in upregulated genes, but not in downregulated genes. Overlapping gene regulation was especially evident for genes and pathways associated with extracellular matrix remodeling. Interestingly, the genes most highly induced by cold acclimation were involved in contraction and in signal transduction between nerve and muscle cells, while no significant changes were observed in genes and pathways related to insulin signaling or glucose metabolism. Conclusions: Overall, our results indicate that cold acclimation and exercise training have overlapping effects on gene expression in human skeletal muscle, but strikingly these overlapping genes are designated to pathways related to cell remodeling rather than metabolic pathways.
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| Overall design |
Microarray analysis was performed on a muscle biopsies taken from overweight middle-aged male subjects before and after a 12 week combined exercise training intervention.
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| Contributor(s) |
Nascimento EB, Hangelbroek RW, Hooiveld GJ, Hoeks J, Van Marken Lichtenbelt WD, Hesselink MH, Schrauwen P, Kersten S |
| Citation(s) |
32887608 |
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| Submission date |
Aug 14, 2020 |
| Last update date |
Sep 21, 2020 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
[email protected]
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| Organization name |
Wageningen University
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| Department |
Div. Human Nutrition & Health
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| Lab |
Nutrition, Metabolism & Genomics Group
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| Street address |
HELIX, Stippeneng 4
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| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL11532 |
[HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (36)
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| GSM4727551 |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject GL23 |
| GSM4727552 |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject RJ28 |
| GSM4727553 |
muscly biopsy, healthy overweight middle-aged male subject, after training intervention, subject GL23 |
| GSM4727554 |
muscly biopsy, healthy overweight middle-aged male subject, after training intervention, subject RJ28 |
| GSM4727555 |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject RP12 |
| GSM4727556 |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject HE24 |
| GSM4727557 |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject JW34 |
| GSM4727558 |
muscly biopsy, healthy overweight middle-aged male subject, after training intervention, subject RP12 |
| GSM4727559 |
muscly biopsy, healthy overweight middle-aged male subject, after training intervention, subject HE24 |
| GSM4727560 |
muscly biopsy, healthy overweight middle-aged male subject, after training intervention, subject JW34 |
| GSM4727561 |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject LD21 |
| GSM4727562 |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject GS25 |
| GSM4727563 |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject LB36 |
| GSM4727564 |
muscly biopsy, healthy overweight middle-aged male subject, after training intervention, subject LD21 |
| GSM4727565 |
muscly biopsy, healthy overweight middle-aged male subject, after training intervention, subject GS25 |
| GSM4727566 |
muscly biopsy, healthy overweight middle-aged male subject, after training intervention, subject LB36 |
| GSM4727567 |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject JS22 |
| GSM4727568 |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject LC26 |
| GSM4727569 |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject FL37 |
| GSM4727570 |
muscly biopsy, healthy overweight middle-aged male subject, after training intervention, subject JS22 |
| GSM4727571 |
muscly biopsy, healthy overweight middle-aged male subject, after training intervention, subject LC26 |
| GSM4727572 |
muscly biopsy, healthy overweight middle-aged male subject, after training intervention, subject FL37 |
| GSM4727573 |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject RT1 |
| GSM4727574 |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject DG51 |
| GSM4727575 |
muscly biopsy, healthy overweight middle-aged male subject, after training intervention, subject RT1 |
| GSM4727576 |
muscly biopsy, healthy overweight middle-aged male subject, after training intervention, subject DG51 |
| GSM4727577 |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject RN38 |
| GSM4727578 |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject PP58 |
| GSM4727579 |
muscly biopsy, healthy overweight middle-aged male subject, after training intervention, subject RN38 |
| GSM4727580 |
muscly biopsy, healthy overweight middle-aged male subject, after training intervention, subject PP58 |
| GSM4727581 |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject RS59 |
| GSM4727582 |
muscly biopsy, healthy overweight middle-aged male subject, after training intervention, subject RS59 |
| GSM4727583 |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject BE50 |
| GSM4727584 |
muscly biopsy, healthy overweight middle-aged male subject, before training intervention, subject HM67 |
| GSM4727585 |
muscly biopsy, healthy overweight middle-aged male subject, after training intervention, subject BE50 |
| GSM4727586 |
muscly biopsy, healthy overweight middle-aged male subject, after training intervention, subject HM67 |
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| This SubSeries is part of SuperSeries: |
| GSE156249 |
Comparative transcriptome analysis of human skeletal muscle in response to cold acclimation and exercise training in human volunteers. |
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| Relations |
| BioProject |
PRJNA657186 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE156247_RAW.tar |
150.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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